PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0895 [new locus tag: SAUSA300_RS04825 ]
• pan locus tag^?: SAUPAN003152000
• symbol: oppB
• pan gene symbol^?: opp4B
• synonym:
• product: oligopeptide ABC transporter permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0895 [new locus tag: SAUSA300_RS04825 ]
• symbol: oppB
• product: oligopeptide ABC transporter permease
• replicon: chromosome
• strand: +
• coordinates: 983789..984751
• length: 963
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913893 NCBI
• RefSeq: YP_493595 NCBI
• BioCyc: see SAUSA300_RS04825
• MicrobesOnline: 1292410 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

• 1
  61
  121
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  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  
  ATGATTAAATTAATACTTAAAAGATTAGGGTTAATGATCCCGTTACTAATTTTAATCTCA
  ATAGTTGTATTTTCATTAGCAATCATACAACCAGGAGATCCATTTTCAGATCTACAAAAC
  GGTAAAATAAAACAAGAAGCAATAAATGCACAAAGGGAAAAATTAGGTCTCAACGACTCA
  ATACCACATCAATACATTAGATGGGTCAATCATGTTATACACGGTGATTTAGGTGAGTCA
  ATCAAATATAAAAGGCCGGTAATTGATGTTATTGAGGAAAGAATTCCAAATACAATATTA
  CTCGGTGCTATGTCATTAATTATTACTTATATTATCTCATTTGCTTTAGGAATAACGTCA
  GGTAGATATTCTTACAGTTTGACGGATTATACTGTGCAAATATTTAATTATTTGATGTTA
  GCCATTCCATCTTTTATTGCGGGAGTATTTGCAATTTTTATTTTTTCTTTTGAATTACAA
  TGGTTCCCGTTTCAAGGTTCTGTTGATATTAACCTTAAAGAAGGTACTTTTGAATATTAT
  ATGAGTAAAATATATCACACATTTTTACCTGCATTCACTTTAGGTTTATTATCTACTGCT
  GGTTATATTCAATATTTACGTAATGATATTATTGAAAATTCTAAAAAAGATTATGTATTG
  ACGGCAAGGTCAAAAGGATTATCTATGAATAAAATTTATAATAAACATATATTGAGAAAT
  TCTCTAATACCTATTATTACATTTTTAGGTGCTGATATTGTAAGTATTTTAGGTGGAGCC
  GTGATTACGGAGACTATCTTTTCATATAACGGTATCGGTAAATTATTTTTAGAATCGGTA
  ATAGGCCAAGACTATCCATTAATGATGGCATTAACGTTATTTTTCTCATTTTTAGGTTTA
  CTGGGTAATTTGATTTCTGATATTACTTATGGATTTATAGATCCAAGAATTAGAAGTAAC
  TAG
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  963
  

⊟Protein

⊟General

• locus tag: SAUSA300_0895 [new locus tag: SAUSA300_RS04825 ]
• symbol: OppB
• description: oligopeptide ABC transporter permease
• length: 320
• theoretical pI: 8.94727
• theoretical MW: 36123.3
• GRAVY: 0.459062

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikB (TIGR02789; EC 3.6.3.24; HMM-score: 236.8)
  and 3 more
  Metabolism Transport and binding proteins Cations and iron carrying compounds nickel ABC transporter, permease subunit NikC (TIGR02790; EC 3.6.3.24; HMM-score: 23.4)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstC (TIGR02138; HMM-score: 18)
  Metabolism Transport and binding proteins Anions phosphate ABC transporter, permease protein PstA (TIGR00974; HMM-score: 15.3)
• TheSEED  :
  • Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1)
  Membrane Transport ABC transporters ABC transporter oligopeptide (TC 3.A.1.5.1)  Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1)
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 138.5)
  and 1 more
  IT (CL0182) CitMHS; Citrate transporter (PF03600; HMM-score: 11.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 6
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: 0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: Signal peptide SP(Sec/SPI) length 27 aa
  • SP(Sec/SPI): 0.517601
  • TAT(Tat/SPI): 0.001512
  • LIPO(Sec/SPII): 0.035073
  • Cleavage Site: CS pos: 27-28. SLA-II. Pr: 0.1516
• predicted transmembrane helices (TMHMM): 6

⊟Accession numbers

• GI: 87161748 NCBI
• RefSeq: YP_493595 NCBI
• UniProt: A0A0H2XHQ9 UniProt

⊟Protein sequence

• MIKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIKQEAINAQREKLGLNDSIPHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLGAMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQWFPFQGSVDINLKEGTFEYYMSKIYHTFLPAFTLGLLSTAGYIQYLRNDIIENSKKDYVLTARSKGLSMNKIYNKHILRNSLIPIITFLGADIVSILGGAVITETIFSYNGIGKLFLESVIGQDYPLMMALTLFFSFLGLLGNLISDITYGFIDPRIRSN

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: oppA > oppF > oppD > oppB > oppC

⊟Regulation

• regulator: CodY (repression) regulon

  CodY

  (TF)

  important in Amino acid metabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications