PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0748 [new locus tag: SAUSA300_RS04040 ]
• pan locus tag^?: SAUPAN002689000
• symbol: SAUSA300_0748
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0748 [new locus tag: SAUSA300_RS04040 ]
• symbol: SAUSA300_0748
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 835045..835956
• length: 912
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3914919 NCBI
• RefSeq: YP_493450 NCBI
• BioCyc: see SAUSA300_RS04040
• MicrobesOnline: 1292263 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  901
  
  ATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCT
  GGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGAT
  AATCTACCACCAGTGTTATTGCCTAAATTTGTAGAGTTGATGGAACAAGGAAATCCATCC
  TTAAGAAAAGTGGCAATTGCAATTGATTTAAGAGGTAAGGAACTATTTAATTCATTAGTT
  GCAGTAGTGGATAAAGTCAAAAGTGAAAGTGACGTCATCATTGATGTTATGTTTTTAGAA
  GCAAGTACTGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATG
  GAACAAGGTAAAAGATCGTTAATCAATGCAATTAATGATGAGCGAGAGCATTTGTCTCAA
  ATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAATTAAAA
  GAACGCATTCGTCGATACTATGAAGATGAAGAGTTTGAAACTTTTACAATTAATGTCACA
  AGTTTCGGTTTTAAACATGGGATTCAGATGGATGCAGATTTAGTATTTGATGTACGATTT
  TTACCAAATCCATATTATGTAGTAGATTTAAGACCTTTAACAGGATTAGATAAAGACGTT
  TATAATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTG
  TTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATC
  GGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTA
  AATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTGGC
  GAGAAAAAATGA
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⊟Protein

⊟General

• locus tag: SAUSA300_0748 [new locus tag: SAUSA300_RS04040 ]
• symbol: SAUSA300_0748
• description: hypothetical protein
• length: 303
• theoretical pI: 5.71204
• theoretical MW: 34811.8
• GRAVY: -0.30066

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Anions phosphonate ABC transporter, ATP-binding protein (TIGR02315; EC 3.6.3.28; HMM-score: 15.2)
  Metabolism Central intermediary metabolism Sulfur metabolism adenylyl-sulfate kinase (TIGR00455; EC 2.7.1.25; HMM-score: 14.7)
• TheSEED  :
  • RNase adapter protein RapZ
  Experimental tye  Hypothetical ATP-binding protein UPF0042, contains P-loop
• PFAM:
  P-loop_NTPase (CL0023) ATP_bind_2; P-loop ATPase protein family (PF03668; HMM-score: 360)
  and 10 more
  AAA_18; AAA domain (PF13238; HMM-score: 16.7)
  AAA_22; AAA domain (PF13401; HMM-score: 16.3)
  SKI; Shikimate kinase (PF01202; HMM-score: 16.1)
  DnaB_C; DnaB-like helicase C terminal domain (PF03796; HMM-score: 15.4)
  no clan defined PGM_PMM_II; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II (PF02879; HMM-score: 14.8)
  P-loop_NTPase (CL0023) ABC_tran; ABC transporter (PF00005; HMM-score: 14.7)
  AAA_16; AAA ATPase domain (PF13191; HMM-score: 14.7)
  no clan defined DUF1186; Protein of unknown function (DUF1186) (PF06685; HMM-score: 14.5)
  P-loop_NTPase (CL0023) AAA_33; AAA domain (PF13671; HMM-score: 14.5)
  RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 12.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.017699
  • TAT(Tat/SPI): 0.006084
  • LIPO(Sec/SPII): 0.015606
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160086 NCBI
• RefSeq: YP_493450 NCBI
• UniProt: Q2FIM8 UniProt

⊟Protein sequence

• MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPPVLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEASTEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELKERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDKDVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERLGNYLNEVFEYNVYVHHRDAHIESGEKK

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0748 > SAUSA300_0749 > SAUSA300_0750

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications