PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0464 [new locus tag: SAUSA300_RS02490 ]
• pan locus tag^?: SAUPAN002223000
• symbol: SAUSA300_0464
• pan gene symbol^?: —
• synonym:
• product: hypothetical protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0464 [new locus tag: SAUSA300_RS02490 ]
• symbol: SAUSA300_0464
• product: hypothetical protein
• replicon: chromosome
• strand: +
• coordinates: 523696..524421
• length: 726
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913248 NCBI
• RefSeq: YP_493173 NCBI
• BioCyc: see SAUSA300_RS02490
• MicrobesOnline: 1291979 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGTTAAAAGAGAATGAACGATTTGATCAACTAATCAAAGAAGATTTTAGTATTATTCAA
  AATGATGATGTTTTTTCATTTTCAACGGATGCTTTGTTGTTAGGGCATTTTACAAAACCT
  AGAACAAAAGATATTGTGTTGGACTTATGTTCAGGCAATGGGGTGATACCCTTGTTATTG
  TTTGCGAAACATCCACGACATATAGAAGGTGTTGAGATTCAAAAAACACTTGTCGATATG
  GCGCGACGCACATTTCAATTCAATGATGTTGATGAATATTTAACAATGCATCACATGGAT
  TTGAAAAACGTTACTAAAGTATTTAAACCTTCACAATATACTTTAGTAACGTGTAATCCG
  CCTTATTTTAAAGAGAATCAGCAACACCAACATCAAAAAGAAGCACATAAGATAGCGAGA
  CATGAGATTATGTGTACACTTGAAGATTGCATGATTGCAGCCCGTCATTTATTAAAAGAA
  GGTGGCAGGCTAAACATGGTACATCGTGCAGAGAGACTAATGGATGTCTTGTTTGAAATG
  AGAAAAGTGAATATTGAACCTAAGAAAGTCGTTTTTATATATAGTAAAGTAGGGAAATCA
  GCACAAACGATAGTAGTAGAAGGTCGAAAAGGTGGAAATCAAGGTTTAGAAATCATGCCC
  CCATTTTATATTTATAATGAAGATGGTAATTATAGCGAAGAAATGAAGGAAGTATATTAT
  GGATAG
  60
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  726
  

⊟Protein

⊟General

• locus tag: SAUSA300_0464 [new locus tag: SAUSA300_RS02490 ]
• symbol: SAUSA300_0464
• description: hypothetical protein
• length: 241
• theoretical pI: 7.10892
• theoretical MW: 28096.3
• GRAVY: -0.438589

⊟Function

• TIGRFAM:
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 45.6)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 37.6)
  and 7 more
  Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 36.4)
  Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 23.3)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 15.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 14.8)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 12.9)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification putative protein-(glutamine-N5) methyltransferase, unknown substrate-specific (TIGR03704; EC 2.1.1.-; HMM-score: 12.7)
  Metabolism Amino acid biosynthesis Aspartate family methionine biosynthesis protein MetW (TIGR02081; HMM-score: 11.8)
• TheSEED  :
  • COG4123: Predicted O-methyltransferase
  Stress Response Heat shock Heat shock dnaK gene cluster extended  COG4123: Predicted O-methyltransferase
• PFAM:
  NADP_Rossmann (CL0063) MTS; Methyltransferase small domain (PF05175; HMM-score: 56.8)
  and 11 more
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 33.5)
  Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 24.9)
  Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 20.5)
  UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 19.6)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 18.4)
  Methyltransf_10; Protein of unknown function (DUF890) (PF05971; HMM-score: 18.3)
  Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 18.3)
  N6-adenineMlase; Probable N6-adenine methyltransferase (PF10237; HMM-score: 17.3)
  Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 17.1)
  Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 14.8)
  PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 13.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00241
  • TAT(Tat/SPI): 0.000678
  • LIPO(Sec/SPII): 0.000779
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160180 NCBI
• RefSeq: YP_493173 NCBI
• UniProt: A0A0H2XE11 UniProt

⊟Protein sequence

• MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKDIVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLKNVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKEGGRLNMVHRAERLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEIMPPFYIYNEDGNYSEEMKEVYYG

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0464 > SAUSA300_0465 > SAUSA300_0466

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications