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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_00457
- pan locus tag?: SAUPAN002223000
- symbol: SAOUHSC_00457
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SAOUHSC_00457
- symbol: SAOUHSC_00457
- product: hypothetical protein
- replicon: chromosome
- strand: +
- coordinates: 459625..460350
- length: 726
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3920317 NCBI
- RefSeq: YP_499036 NCBI
- BioCyc: G1I0R-424 BioCyc
- MicrobesOnline: 1288934 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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721ATGTTAAAAGAGAATGAACGATTTGATCAACTAATCAAAGAAGATTTTAGTATTATTCAA
AATGATGATGTTTTTTCATTTTCAACGGATGCTTTGTTGTTAGGGCATTTTACAAAACCT
AGAACAAAAGATATTGTGTTGGACTTATGTTCAGGCAATGGGGTGATACCCTTGTTATTG
TTTGCGAAACATCCACGACATATAGAAGGTGTTGAGATTCAAAAAACACTTGTCGATATG
GCGCGACGCACATTTCAATTCAATGATGTTGATGAATATTTAACAATGCATCACATGGAT
TTGAAAAACGTTACTAAAGTATTTAAACCTTCACAATATACTTTAGTAACGTGTAATCCG
CCTTATTTTAAAGAGAATCAGCAACACCAACATCAAAAAGAAGCACATAAGATAGCGAGA
CATGAGATTATGTGTACACTTGAAGATTGCATGATTGCAGCCCGTCATTTATTAAAAGAA
GGTGGCAGGCTAAACATGGTACATCGTGCAGAGAGACTAATGGATGTCTTGTTTGAAATG
AGAAAAGTGAATATTGAACCTAAGAAAGTCGTTTTTATATATAGTAAAGTAGGGAAATCA
GCACAAACGATAGTAGTAGAAGGTCGAAAAGGTGGAAATCAAGGTTTAGAAATCATGCCC
CCATTTTATATTTATAATGAAGATGGTAATTATAGCGAAGAAATGAAGGAAGTATATTAT
GGATAG60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_00457
- symbol: SAOUHSC_00457
- description: hypothetical protein
- length: 241
- theoretical pI: 7.10892
- theoretical MW: 28096.3
- GRAVY: -0.438589
⊟Function[edit | edit source]
- TIGRFAM: Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 45.6)Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 37.6)and 7 moreProtein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 36.4)Unknown function Enzymes of unknown specificity putative methylase (TIGR00537; HMM-score: 23.3)Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 15.5)Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 14.8)Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 12.9)Protein synthesis Ribosomal proteins: synthesis and modification putative protein-(glutamine-N5) methyltransferase, unknown substrate-specific (TIGR03704; EC 2.1.1.-; HMM-score: 12.7)Amino acid biosynthesis Aspartate family methionine biosynthesis protein MetW (TIGR02081; HMM-score: 11.8)
- TheSEED :
- COG4123: Predicted O-methyltransferase
- PFAM: NADP_Rossmann (CL0063) MTS; Methyltransferase small domain (PF05175; HMM-score: 56.8)and 11 moreMethyltransf_25; Methyltransferase domain (PF13649; HMM-score: 33.5)Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 24.9)Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 20.5)UPF0020; Putative RNA methylase family UPF0020 (PF01170; HMM-score: 19.6)Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 18.4)Methyltransf_10; Protein of unknown function (DUF890) (PF05971; HMM-score: 18.3)Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 18.3)N6-adenineMlase; Probable N6-adenine methyltransferase (PF10237; HMM-score: 17.3)Methyltransf_23; Methyltransferase domain (PF13489; HMM-score: 17.1)Methyltransf_4; Putative methyltransferase (PF02390; HMM-score: 14.8)PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 13.2)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.00241
- TAT(Tat/SPI): 0.000678
- LIPO(Sec/SPII): 0.000779
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKDIVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYLTMHHMDLKNVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKEGGRLNMVHRAERLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEIMPPFYIYNEDGNYSEEMKEVYYG
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [1] [2]
- protein localization: data available for COL
- quantitative data / protein copy number per cell:
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SAOUHSC_00457 > SAOUHSC_00458 > SAOUHSC_00459predicted SigA promoter [3] : SAOUHSC_00452 > S148 > SAOUHSC_00454 > SAOUHSC_00455 > SAOUHSC_00456 > S149 > SAOUHSC_00457predicted SigA promoter [3] : SAOUHSC_00452 > S148 > SAOUHSC_00454 > SAOUHSC_00455 > SAOUHSC_00456 > S149 > SAOUHSC_00457 > SAOUHSC_00458 > SAOUHSC_00459 > S150 > SAOUHSC_00461 > SAOUHSC_00462 > S151 > SAOUHSC_00463 > ksgA > S152
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [3]
Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 3.0 3.1 3.2 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)