From AureoWiki
Jump to navigation Jump to search

NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus USA300_FPR3757
  • locus tag: SAUSA300_0319 [new locus tag: SAUSA300_RS01700 ]
  • pan locus tag?: SAUPAN001243000
  • symbol: SAUSA300_0319
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAUSA300_0319 [new locus tag: SAUSA300_RS01700 ]
  • symbol: SAUSA300_0319
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 370684..371997
  • length: 1314
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAATCAATATCATTCTAATGCACAACAACCAAGTGCATGGCGTTTTTTTGTCTATAGT
    TTAGTGGGCATACTATGTTTCTTTATTCCTTTTACGATTAATGGTAACAACACTATTTTC
    GTCGATCATGTTCATCTAGCCATTCGCTCAATCATAGGTCCACTTATGCCCTATGTTGCA
    CTGATTATGATTTTAATTGGTACAGCGTTACCAATAGTGAGACGTACTTTTATGACTTCA
    ATCACAAACTTGGTCATTACATTATTTAAAGTTGCAGGTGCAATGATCGGTATAATGTAT
    GTATTTAAAATCGGTCCATCAATACTATTTAAAGCTAACTATGGTCCGTTTTTGTTTGAA
    AAATTAATGATGCCATTAAGTATCTTAATTCCAGTAGGTGCAATTGCGCTTTCTTTATTA
    GTGGGCTATGGCTTATTAGAATTTGTCGGTGTTTATATGGAGCCTATTATGAGACCTATT
    TTTAAAACACCAGGAAAATCCGCTGTCGATGCAGTGGCTTCGTTTGTCGGCAGTTATTCC
    TTAGGATTATTGATTACTAATCGTGTCTATAAGCAAGGGATGTACAACAAACGAGAAGCC
    ACGATTATTGCGACTGGCTTTTCAACAGTTTCAGCAACTTTTATGATTATCGTTGCTAAA
    ACTTTAGGATTAATGCCGCATTGGAATTTATACTTTTGGATAACTTTAGTCATCACATTT
    GTCGTGACTGCAATTACTGCATGGCTACCGCCAATCAGCAATGAATCAACAGAATATTAT
    AACGGACAAGAAGGAGAACAAGAAGTTGCTATTGAAGGAAGCAGACTGAAAACTGCATAT
    GCAGAGGCGATGAAACAAAATGCATTAACACCATCTCTCGTGAAGAACGTTTGGGACAAT
    TTGAAAGACGGTTTAGAAATGACTGTTGGTATTTTACCTTCTATATTATCGATTGGTTTT
    TTAGGACTGATTGTAGCGAACTATACACCATTCATTGATTGGCTTGGCTATATCTTCTAT
    CCATTTATTTATATTTTCCCAATTGCTGATCAGGCTTTACTAGCAAAAGCGTCAGCGATT
    TCTATTGTAGAGATGTTTCTACCATCTTTGTTAGTAACTAAAGCTGCAATGAGTACTAAA
    TTTGTCGTCGGTGTAGTAAGCGTATCAGCCATTATCTTTTTCTCAGCATTAGTGCCATGT
    ATACTAGCAACTGAAATTAAAATACCTGTCTGGAAACTCATCATCATTTGGTTTTTACGC
    GTGGCGTTGTCGCTATTAATCACCATCCCCGTCGCTTTACTTATTTTTGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1314

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAUSA300_0319 [new locus tag: SAUSA300_RS01700 ]
  • symbol: SAUSA300_0319
  • description: hypothetical protein
  • length: 437
  • theoretical pI: 9.47963
  • theoretical MW: 48281.6
  • GRAVY: 0.881693

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Sporulation and germination sporulation integral membrane protein YlbJ (TIGR02871; HMM-score: 28.4)
  • TheSEED  :
    • Predicted histidine uptake transporter
  • PFAM:
    no clan defined Gate; Nucleoside recognition (PF07670; HMM-score: 37.1)
    and 1 more
    DUF3742; Protein of unknown function (DUF3742) (PF12553; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.02089
    • TAT(Tat/SPI): 0.000385
    • LIPO(Sec/SPII): 0.018297
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHVHLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVFKIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPIFKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIVAKTLGLMPHWNLYFWITLVITFVVTAITAWLPPISNESTEYYNGQEGEQEVAIEGSRLKTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDWLGYIFYPFIYIFPIADQALLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAIIFFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: HutR* (activation) regulon
    HutR*(TF)important in Histidine utilization; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAUSA300_0319&oldid=99489"