From AureoWiki
Jump to navigation Jump to search

NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_00299
  • pan locus tag?: SAUPAN001243000
  • symbol: SAOUHSC_00299
  • pan gene symbol?: —
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_00299
  • symbol: SAOUHSC_00299
  • product: hypothetical protein
  • replicon: chromosome
  • strand: -
  • coordinates: 312595..313908
  • length: 1314
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGAATCAATATCATTCTAATGCACAACAACCAAGTGCATGGCGTTTTTTTGTCTATAGT
    TTAGTGGGCATACTATGTTTCTTTATTCCTTTTACGATTAATGGTAACAACACTATTTTC
    GTCGATCATGTTCATCTAGCCATTCGCTCAATCATAGGTCCACTTATGCCCTATGTTGCA
    CTGATTATGATTTTAATTGGTACAGCGTTACCAATAGTGAGACGTACTTTTATGACTTCA
    ATCACAAACTTGGTCATTACATTATTTAAAGTTGCAGGTGCAATGATCGGTATAATGTAT
    GTATTTAAAATCGGTCCATCAATACTATTTAAAGCTAACTATGGTCCGTTTTTGTTTGAA
    AAATTAATGATGCCATTAAGTATCTTAATTCCAGTAGGTGCAATTGCGCTTTCTTTATTA
    GTGGGCTATGGCTTATTAGAATTTGTCGGTGTTTATATGGAGCCTATTATGAGACCTATT
    TTTAAAACACCAGGAAAATCCGCTGTCGATGCAGTGGCTTCGTTTGTCGGCAGTTATTCC
    TTAGGATTATTGATTACTAATCGTGTCTATAAGCAAGGGATGTACAACAAACGAGAAGCC
    ACGATTATTGCGACTGGCTTTTCAACAGTTTCAGCAACTTTTATGATTATCGTTGCTAAA
    ACTTTAGGATTAATGCCGCATTGGAATTTATACTTTTGGATAACTTTAGTCATCACATTT
    GTCGTGACTGCAATTACTGCATGGCTACCGCCAATCAGCAATGAATCAACAGAATATTAT
    AACGGACAAGAAGGAGAACAAGAAGTTGCTATTGAAGGAAGCAGACTGAAAACTGCATAT
    GCAGAGGCGATGAAACAAAATGCATTAACACCATCTCTCGTGAAGAACGTTTGGGACAAT
    TTGAAAGACGGTTTAGAAATGACTGTTGGTATTTTACCTTCTATATTATCGATTGGTTTT
    TTAGGACTGATTGTAGCGAACTATACACCATTCATTGATTGGCTTGGCTATATCTTCTAT
    CCATTTATTTATATTTTCCCAATTGCTGATCAGGCTTTACTAGCAAAAGCGTCAGCGATT
    TCTATTGTAGAGATGTTTCTACCATCTTTGTTAGTAACTAAAGCTGCAATGAGTACTAAA
    TTTGTCGTCGGTGTAGTAAGCGTATCAGCCATTATCTTTTTCTCAGCATTAGTGCCATGT
    ATACTAGCAACTGAAATTAAAATACCTGTCTGGAAACTCATCATCATTTGGTTTTTACGC
    GTGGCGTTGTCGCTATTAATCACCATCCCCGTCGCTTTACTTATTTTTGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1314

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_00299
  • symbol: SAOUHSC_00299
  • description: hypothetical protein
  • length: 437
  • theoretical pI: 9.47963
  • theoretical MW: 48281.6
  • GRAVY: 0.881693

⊟Function[edit | edit source]

  • TIGRFAM:
    Cellular processes Cellular processes Sporulation and germination sporulation integral membrane protein YlbJ (TIGR02871; HMM-score: 28.4)
  • TheSEED  :
    • Predicted histidine uptake transporter
  • PFAM:
    no clan defined Gate; Nucleoside recognition (PF07670; HMM-score: 37.1)
    and 1 more
    DUF3742; Protein of unknown function (DUF3742) (PF12553; HMM-score: 13.8)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 12
  • LocateP: Multi-transmembrane
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.02089
    • TAT(Tat/SPI): 0.000385
    • LIPO(Sec/SPII): 0.018297
  • predicted transmembrane helices (TMHMM): 12

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHVHLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVFKIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPIFKTPGKSAVDAVASFVGSYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIVAKTLGLMPHWNLYFWITLVITFVVTAITAWLPPISNESTEYYNGQEGEQEVAIEGSRLKTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDWLGYIFYPFIYIFPIADQALLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAIIFFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: HutR* (activation) regulon
    HutR*(TF)important in Histidine utilization; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=SAOUHSC_00299&oldid=55436"