PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0176 [new locus tag: SAUSA300_RS00925 ]
• pan locus tag^?: SAUPAN001012000
• symbol: SAUSA300_0176
• pan gene symbol^?: ssuC
• synonym:
• product: ABC transporter permease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0176 [new locus tag: SAUSA300_RS00925 ]
• symbol: SAUSA300_0176
• product: ABC transporter permease
• replicon: chromosome
• strand: +
• coordinates: 198053..198814
• length: 762
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3913227 NCBI
• RefSeq: YP_492890 NCBI
• BioCyc: see SAUSA300_RS00925
• MicrobesOnline: 1291691 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGACACGTCCCACAAATAACAAATTTATATTACCTATTATCACATTTATTATTTTCTTA
  GGCATTTGGGAAATGGTCATTATTATTGGGCATTACCAACCTGTATTGTTACCGGGTCCT
  GCTCTTGTAGGAAAAAGTATATGGTCTTTCATTGTTACTGGAGAAATTTTCCAACATTTA
  GCAATTAGTTTATGGAGATTTGTAGCGGGCTTTGTTGTCGCATTGTTGGTTGCTATTCCA
  TTGGGCTTCTTGCTTGGAAGGAATCGTTGGCTATACAACGCTATCGAACCGCTATTTCAA
  TTGATTAGGCCGATATCTCCGATAGCATGGGCACCATTTGTTGTTCTATGGTTTGGTATT
  GGTAGTTTGCCAGCGATTGCGATTATTTTTATCGCTGCTTTTTTCCCAATTGTGTTCAAT
  ACTATTAAAGGCGTTAGAGACATTGAACCTCAATATTTAAAAATAGCAGCAAATTTAAAT
  TTAACTGGGTGGTCATTGTATCGCAATATATTATTTCCCGGGGCATTTAAACAAATCATG
  GCTGGGATACATATGGCGGTAGGAACAAGTTGGATATTTTTAGTTTCTGGTGAAATGATT
  GGTGCACAATCGGGATTAGGTTTTTTAATCGTTGATGCACGAAATATGTTGAACTTAGAA
  GATGTTTTAGCAGCAATATTCTTTATCGGATTATTTGGTTTTATTATTGATCGATTCATT
  AGTTATATTGAGCAGTTTATACTTAGAAGATTTGGTGAATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0176 [new locus tag: SAUSA300_RS00925 ]
• symbol: SAUSA300_0176
• description: ABC transporter permease
• length: 253
• theoretical pI: 10.0243
• theoretical MW: 28431.9
• GRAVY: 0.969565

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Anions nitrate ABC transporter, permease protein (TIGR01183; HMM-score: 140.3)
  and 4 more
  choline ABC transporter, permease protein (TIGR03416; HMM-score: 61.9)
  NifC-like ABC-type porter (TIGR01581; HMM-score: 27.8)
  Metabolism Transport and binding proteins Anions phosphonate ABC transporter, permease protein PhnE (TIGR01097; HMM-score: 26.4)
  2-aminoethylphosphonate ABC transporter, permease protein (TIGR03226; HMM-score: 21.5)
• TheSEED  :
  • ABC transporter permease protein with unknown substrate
• PFAM:
  BPD_transp_1 (CL0404) BPD_transp_1; Binding-protein-dependent transport system inner membrane component (PF00528; HMM-score: 83.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 5
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010693
  • TAT(Tat/SPI): 0.000275
  • LIPO(Sec/SPII): 0.00392
• predicted transmembrane helices (TMHMM): 6

⊟Accession numbers

• GI: 87161514 NCBI
• RefSeq: YP_492890 NCBI
• UniProt: A0A0H2XGU9 UniProt

⊟Protein sequence

• MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPALVGKSIWSFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLIRPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLNLTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLNLEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_0174 > SAUSA300_0175 > SAUSA300_0176 > SAUSA300_0177

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Olga Soutourina, Sarah Dubrac, Olivier Poupel, Tarek Msadek, Isabelle Martin-Verstraete
The pleiotropic CymR regulator of Staphylococcus aureus plays an important role in virulence and stress response.
PLoS Pathog: 2010, 6(5);e1000894
[PubMed:20485570] [WorldCat.org] [DOI] (I e)