PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0062 [new locus tag: SAUSA300_RS00315 ]
• pan locus tag^?: SAUPAN000811000
• symbol: arcB
• pan gene symbol^?: arcB
• synonym:
• product: ornithine carbamoyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0062 [new locus tag: SAUSA300_RS00315 ]
• symbol: arcB
• product: ornithine carbamoyltransferase
• replicon: chromosome
• strand: -
• coordinates: 69839..70837
• length: 999
• essential: unknown

⊟Accession numbers

• Gene ID: 3914770 NCBI
• RefSeq: YP_492781 NCBI
• BioCyc: see SAUSA300_RS00315
• MicrobesOnline: 1291577 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAAAATTTAAGAAATAGAAACTTTTTAACTTTGTTAGACTTCACACAAAAAGAAATG
  GAATTTTTACTTAATTTATCTGAAGATCTTAAACGCGCAAAATATGCAGGAATAGAACAA
  CAAAAAATGAAAGGTAAAAATATCGCTCTACTTTTTGAAAAAGATTCAACACGCACTCGA
  TGTGCATTTGAAACAGCGGCTTATGATCAAGGTGCACATGTAACATACCTTGGGCCAACA
  GGTTCTCAAATGGGTAAAAAAGAGTCTACCAAAGATACTGCTCGTGTTTTAGGTGGAATG
  TATGATGGCATAGAATATCGTGGATTCTCACAAAGAGTAGTTGAGGATTTGGCAAAATAT
  TCTGGCGTTCCCGTATGGAATGGTTTAACAGATGAAGATCATCCTACACAAGTACTTGCT
  GATTTTTTAACAGCTAAAGAAGTATTGAAAAAACCATATAATGAAATTAACTTCACATAT
  GTTGGAGATGGACGTAATAACGTTGCAAATGCCTTAATGCAAGGAGCAGCAATCATGGGC
  ATGACTTTCCATCTTGTATGTCCTAAAGAACTAAATCCAACTGATGAATTATTAAATCGG
  TGTAATGATATAGCTGACAAAAACGGTGGTGAAATCCTTATAACTGATGATATTGATGAA
  GGTGTCAAAGGATCTGATGTAATTTACACAGATGTTTGGGTATCTATGGGTGAACCTGAT
  GAAGTTTGGGAAAAACGTATCAAATTATTAGAACCATACCGTGTAACTAAAGAATTAATG
  AAAAAAACAGGTAATCCACATACAATTTTTGAACATTGCTTACCATCATTCCATGATACT
  GAAACAATAATCGGTAAACAAATTCAAGAAAAATATGGTCTAACTGAAATGGAAGTAACA
  AATGAAGTATTTGAAAGTGAACAATCAGTTGTTTTCCAAGAAGCAGAAAATAGAGCACAT
  ACAATAAAAGCAGTCATGGTAGCAACATTAGGAGAGTAA
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⊟Protein

⊟General

• locus tag: SAUSA300_0062 [new locus tag: SAUSA300_RS00315 ]
• symbol: ArcB
• description: ornithine carbamoyltransferase
• length: 332
• theoretical pI: 4.83949
• theoretical MW: 37512.3
• GRAVY: -0.478313

⊟Function

• reaction:
  EC 2.1.3.3?  ExPASy
  Ornithine carbamoyltransferase Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline
• TIGRFAM:
  Metabolism Amino acid biosynthesis Glutamate family ornithine carbamoyltransferase (TIGR00658; EC 2.1.3.3; HMM-score: 405.7)
  and 3 more
  Metabolism Energy metabolism Amino acids and amines putrescine carbamoyltransferase (TIGR04384; EC 2.1.3.6; HMM-score: 264.9)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis aspartate carbamoyltransferase (TIGR00670; EC 2.1.3.2; HMM-score: 118.1)
  knotted carbamoyltransferase YgeW (TIGR03316; EC 2.1.3.-; HMM-score: 68.1)
• TheSEED  :
  • Ornithine carbamoyltransferase (EC 2.1.3.3)
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine and Ornithine Degradation  Ornithine carbamoyltransferase (EC 2.1.3.3)
  and 2 more
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Biosynthesis extended  Ornithine carbamoyltransferase (EC 2.1.3.3)
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Deiminase Pathway  Ornithine carbamoyltransferase (EC 2.1.3.3)
• PFAM:
  Asp-glut_race (CL0399) OTCace; Aspartate/ornithine carbamoyltransferase, Asp/Orn binding domain (PF00185; HMM-score: 186)
  no clan defined OTCace_N; Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding domain (PF02729; HMM-score: 154)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005128
  • TAT(Tat/SPI): 0.001242
  • LIPO(Sec/SPII): 0.000644
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87161371 NCBI
• RefSeq: YP_492781 NCBI
• UniProt: A0A0H2XIR5 UniProt

⊟Protein sequence

• MKNLRNRNFLTLLDFTQKEMEFLLNLSEDLKRAKYAGIEQQKMKGKNIALLFEKDSTRTRCAFETAAYDQGAHVTYLGPTGSQMGKKESTKDTARVLGGMYDGIEYRGFSQRVVEDLAKYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKPYNEINFTYVGDGRNNVANALMQGAAIMGMTFHLVCPKELNPTDELLNRCNDIADKNGGEILITDDIDEGVKGSDVIYTDVWVSMGEPDEVWEKRIKLLEPYRVTKELMKKTGNPHTIFEHCLPSFHDTETIIGKQIQEKYGLTEMEVTNEVFESEQSVVFQEAENRAHTIKAVMVATLGE

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: arcC < arcB < SAUSA300_0063 < arcD < arcA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications