PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_0061 [new locus tag: SAUSA300_RS00310 ]
• pan locus tag^?: SAUPAN000810000
• symbol: arcC
• pan gene symbol^?: arcC
• synonym:
• product: carbamate kinase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_0061 [new locus tag: SAUSA300_RS00310 ]
• symbol: arcC
• product: carbamate kinase
• replicon: chromosome
• strand: -
• coordinates: 68890..69819
• length: 930
• essential: unknown

⊟Accession numbers

• Gene ID: 3912937 NCBI
• RefSeq: YP_492780 NCBI
• BioCyc: see SAUSA300_RS00310
• MicrobesOnline: 1291576 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGTCTAAAATCGTCGTAGCTTTGGGTGGTAACGCTTTAGGACAATCGCCTAAAGAACAA
  TTAGATTTATTAAAAAGTACATCTAAATCTCTAGTAAGTTTAATCGATAAAGGATACGAA
  ATTGTAATTAGTCATGGTAATGGTCCACAAGTTGGAAGTATTAACTTAGGTTTAAATTAT
  GCGGCGGAACACAAACAAGGTCCTCCTTTTCCTTTCCCTGAATGTGGTGCTATGAGTCAA
  GCTTATATTGGCTATCAGATGCAAGAAAGTTTACAAAACGAACTTCATTCAATGGGCATA
  GATAAACAAGTTGTTACGCTAGTAACACAAGTTCAAGTTGCAAGCGATGATTCTGCTTTC
  AATAACCCTACTAAACCAATTGGATTATTTTATACAAAAGAGCAGGCTGATAAATTTACG
  AAAGAAAAAGGTTATACTTTTGTAGAAGATTCTGGACGCGGCTATCGTCGCGTTGTCCCT
  TCCCCACAACCTATAAGTATAGTTGAGTTAGATAGTATAGAAACATTAATCACTCATGGA
  ACACTAGTTATCGCTGCCGGCGGTGGTGGAATTCCAGTAATTAAGGAAAATGAAGTTTAT
  ACAGGTGTTGATGCAGTTATTGATAAAGATAAAACGAGTGCTTTATTAGCAGCACATTTA
  CAATCTGATCAATTAATCATATTAACTGCTGTGGACCATGTTTACATTAACTATGGAAAA
  GAAAACCAAAGAGGTCTCGATGAAGTGTCTGTGGATGAAATGAAAAAACATATCTCTGAT
  GGTCAATTTGCTAAAGGAAGTATGCTTCCAAAAGTTGAAGCTGCACTTCAATTTCTTGAA
  AAAAATACTAAAGGCAGTGTTTTGATTACATCTCTAGCAGGATTAGGGGACGCTTTAGAC
  GGTAAAATAGGAACATTAATTAAGAATTGA
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⊟Protein

⊟General

• locus tag: SAUSA300_0061 [new locus tag: SAUSA300_RS00310 ]
• symbol: ArcC
• description: carbamate kinase
• length: 309
• theoretical pI: 5.37894
• theoretical MW: 33156.5
• GRAVY: -0.145631

⊟Function

• reaction:
  EC 2.7.2.2?  ExPASy
  Carbamate kinase ATP + NH₃ + CO₂ = ADP + carbamoyl phosphate
• TIGRFAM:
  Metabolism Energy metabolism Amino acids and amines carbamate kinase (TIGR00746; EC 2.7.2.2; HMM-score: 378.5)
  and 4 more
  Metabolism Amino acid biosynthesis Glutamate family acetylglutamate kinase (TIGR00761; EC 2.7.2.8; HMM-score: 47.6)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Nucleotide and nucleoside interconversions putative uridylate kinase (TIGR02076; EC 2.7.4.-; HMM-score: 29.6)
  Metabolism Amino acid biosynthesis Glutamate family glutamate 5-kinase (TIGR01027; EC 2.7.2.11; HMM-score: 27.9)
  Metabolism Amino acid biosynthesis Glutamate family delta l-pyrroline-5-carboxylate synthetase (TIGR01092; HMM-score: 20.6)
• TheSEED  :
  • Carbamate kinase (EC 2.7.2.2)
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine and Ornithine Degradation  Carbamate kinase (EC 2.7.2.2)
  and 2 more
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Arginine Deiminase Pathway  Carbamate kinase (EC 2.7.2.2)
  Amino Acids and Derivatives Arginine; urea cycle, polyamines Polyamine Metabolism  Carbamate kinase (EC 2.7.2.2)
• PFAM:
  no clan defined AA_kinase; Amino acid kinase family (PF00696; HMM-score: 112)
  and 1 more
  HAD (CL0137) Hydrolase_3; haloacid dehalogenase-like hydrolase (PF08282; HMM-score: 14.4)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: unknown (no significant prediction)
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 3.33
  • Cellwall Score: 3.33
  • Extracellular Score: 3.33
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 0.83
  • Signal peptide possibility: -0.5
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.030337
  • TAT(Tat/SPI): 0.001273
  • LIPO(Sec/SPII): 0.003738
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 87160434 NCBI
• RefSeq: YP_492780 NCBI
• UniProt: Q2FKJ8 UniProt

⊟Protein sequence

• MSKIVVALGGNALGQSPKEQLDLLKSTSKSLVSLIDKGYEIVISHGNGPQVGSINLGLNYAAEHKQGPPFPFPECGAMSQAYIGYQMQESLQNELHSMGIDKQVVTLVTQVQVASDDSAFNNPTKPIGLFYTKEQADKFTKEKGYTFVEDSGRGYRRVVPSPQPISIVELDSIETLITHGTLVIAAGGGGIPVIKENEVYTGVDAVIDKDKTSALLAAHLQSDQLIILTAVDHVYINYGKENQRGLDEVSVDEMKKHISDGQFAKGSMLPKVEAALQFLEKNTKGSVLITSLAGLGDALDGKIGTLIKN

⊟Experimental data

• experimentally validated:
• protein localization:
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: arcC < arcB < SAUSA300_0063 < arcD < arcA

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications