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⊟Summary[edit | edit source]
- pan ID?: SAUPAN005496000
- symbol?: mtlR
- synonym:
- description?: PTS lactose transporter subunit IIB
- PTS lactose transporter subunit IIB
- BglG family transcriptional antiterminator
- BglG family transcription antiterminator
- BglG-like transcriptional antiterminator, putative
- PRD domain-containing protein
- putative transcriptional regulator
- transcriptional antiterminator BglG family protein
- bglG family mannitol operon activator
- Mannitol operon activator, BglG family
- PTS system, Lactose/Cellobiose specific IIB subunit
- PTS system mannitol (Cryptic)-specific transporter subunit IIA
- putative transcriptional antiterminator
- Transcriptional antiterminator
- transcriptional antiterminator, BglG family/DNA-binding protein
- transcriptional antiterminator BglG
- transcription antiterminator
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 5652695..5654643
- synteny block?: BlockID0042780
- occurrence?: in 100% of 34 strains
mtlR : mannitol operon transcriptional activator MtlR [1]
• the consensus MtlR binding site consists of the inverted repeat sequence : 5'-TTGTCA-n3-WTW-n3-TGACAA-3'
In addition to its role as a carbon source, mannitol is a compatible solute that can impact the osmotic balance of the staphylococcal cell. Therefore, mannitol transportation by phosphotransferase systems can be critical to staphylococcal viability. The transcriptional activator of the mannitol phosphotransferase system (MtlR) is a member of the BglG family of transcriptional activators which can exhibit complex regulation based on phosphorylation status. Extrapolating from the characterized MtlR system in Bacillus stenothermophilus, MtlR can be phosphorylated at HIS-231 or HIS-290 by MtlA resulting in decreased DNA binding and reduced mannitol operon activation. When mannitol is present, MtlA preferentially phosphorylates mannitol and dephosphorylates MtlR resulting in transcriptional activation. MtlR activation can be further enhanced by HPr-mediated phosphorylation of HIS-341 and HIS-398 resulting in increased DNA binding. This ability to calibrate transcriptional activator function based on the availability of substrates is a hallmark of the Escherichia coli antiterminator BglG with PTS regulation domains (PRD-I and PRD-II) serving analogous roles to the HIS-231/290 and HIS-341/398 regulation domains in MtlR. Thus, BglG-family transcriptional activators are often misannotated as antiterminators due to their shared mechanism of regulatory control.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_2096
08BA02176:
C248_2185
11819-97:
MS7_2172
6850:
RSAU_001993
71193:
ST398NM01_2211
ECT-R 2:
ECTR2_2010
ED133:
SAOV_2196
ED98:
SAAV_2211
HO 5096 0412:
SAEMRSA15_20630
JH1:
SaurJH1_2227
JH9:
SaurJH9_2189
JKD6008:
SAA6008_02193 (bglG)
JKD6159:
SAA6159_02066 (bglG)
JSNZ:
JSNZ_002117
LGA251:
SARLGA251_19530
M013:
M013TW_2115
MRSA252:
SAR2245
MSHR1132:
SAMSHR1132_19890
MSSA476:
SAS2058
Mu3:
SAHV_2141
Mu50:
SAV2157
MW2:
MW2083
RF122:
SAB2037
ST398:
SAPIG2211
T0131:
SAT0131_02320
TCH60:
HMPREF0772_11036
TW20:
SATW20_22920
USA300_TCH1516:
USA300HOU_2147 (bglG)
VC40:
SAVC_09650
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKGVEAYLTSFSLTLERAN
N315 MLLSTREKEMIALLIKYHSQYITIHDIAQQLAVSSRTIHRELKGVEAYLTSFSLTLERAN
NCTC8325 MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKGVEAYLTSFSLTLERAN
Newman MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKGVEAYLTSFSLTLERAN
USA300_FPR3757 MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKGVEAYLTSFSLTLERAN
******************.*****************************************
COL KKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALIQAKEPVKQYSLAQEIGVS
N315 KKGLRIAGADSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALIQAKEPVKQYSLAQEIGVS
NCTC8325 KKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALIQAKEPVKQYSLAQEIGVS
Newman KKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALIQAKEPVKQYSLAQEIGVS
USA300_FPR3757 KKGLRIAGTDSDLNDLKQSIAQHQTIDLSVEEQKVIIIYALIQAKEPVKQYSLAQEIGVS
********:***************************************************
COL VQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKREFLSQLMVNNLNSTSVYSVI
N315 VQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKREFLSQLMVNNLNSTSVYSVI
NCTC8325 VQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKREFLSQLMVNNLNSTSVYSVI
Newman VQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKREFLSQLMVNNLNSTSVYSVI
USA300_FPR3757 VQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKREFLSQLMVNNLNSTSVYSVI
************************************************************
COL ENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPYQLTESSYLTLTVHIVLSISRIK
N315 ENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPYQLTESSYLTLTVHIVLSISRIK
NCTC8325 ENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPYQLTESSYLTLTVHIVLSISRIK
Newman ENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPYQLTESSYLTLTVHIVLSISRIK
USA300_FPR3757 ENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPYQLTESSYLTLTVHIVLSISRIK
************************************************************
COL NGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYDVTFNQAEIAFITIHLRGAKRKNLN
N315 NGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYDVTFNQAEIAFITIHLRGAKRKNLN
NCTC8325 NGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYDVTFNQAEIAFITIHLRGAKRKNLN
Newman NGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYDVTFNQAEIAFITIHLRGAKRKNLN
USA300_FPR3757 NGEYVALNDDIYDSVQNTFEHKVASELADKLGQIYDVTFNQAEIAFITIHLRGAKRKNLN
************************************************************
COL DTSLNNRCEENKIKAFVNKVEMISGMTFADLDTLVDGLTLHLNPAINRLQANIETYNPLT
N315 DTSLNNRCEENKIKAFVNKVEMISGMTFADLDTLVDGLTLHLNPAINRLQANIETYNPLT
NCTC8325 DTSLNNRCEENKIKAFVNKVEMISGMTFADLDTLVDGLTLHLNPAINRLQANIETYNPLT
Newman DTSLNNRCEENKIKAFVNKVEMISGMTFADLDTLVDGLTLHLNPAINRLQANIETYNPLT
USA300_FPR3757 DTSLNNRCEENKIKAFVNKVEMISGMTFADLDTLVDGLTLHLNPAINRLQANIETYNPLT
************************************************************
COL DMIKFKYPRLFENVRLALNDCWPDLIFPESEIAFIVLHFGGSIKNQGNRFLNILVVCSSG
N315 DMIKFKYPRLFENVRLALNDCWPDLIFPESEIAFIVLHFGGSIKNQGNRFLNILVVCSSG
NCTC8325 DMIKFKYPRLFENVRLALNDCWPDLIFPESEIAFIVLHFGGSIKNQGNRFLNILVVCSSG
Newman DMIKFKYPRLFENVRLALNDCWPDLIFPESEIAFIVLHFGGSIKNQGNRFLNILVVCSSG
USA300_FPR3757 DMIKFKYPRLFENVRLALNDCWPDLIFPESEIAFIVLHFGGSIKNQGNRFLNILVVCSSG
************************************************************
COL MGTSRLLSTRLEQVFSEIERITQASVSDLKSLDLSQYDGIISTVNLDIDSPYLTVNPLLP
N315 MGTSRLLSTRLEQVFSEIERITQASVSDLKSLDLSQYDGIISTVNLDIDSPYLTVNPLLP
NCTC8325 MGTSRLLSTRLEQVFSEIERITQASVSDLKSLDLSQYDGIISTVNLDIDSPYLTVNPLLP
Newman MGTSRLLSTRLEQVFSEIERITQASVSDLKSLDLSQYDGIISTVNLDIDSPYLTVNPLLP
USA300_FPR3757 MGTSRLLSTRLDQVFSEIERITQASVSDLKSLDLSQYDGIISTVNLDIDSPYLTVNPLLP
***********:************************************************
COL DSDISYVAQFLNTKSTFQETHDKSSNMIDKDDVHVETKDVDGNTSFENEQTSYLTSVFEK
N315 DSDISYVAQFLNTKSTFQETHDKSSNMIDKDDVHVETKDVDGNTSFENEQTSYLTSVFEK
NCTC8325 DSDISYVAQFLNTKSTFQETHDKSSNMIDKDDVHVETKDVDGNTSFENEQTSYLTSVFEK
Newman DSDISYVAQFLNTKSTFQETHDKSSNMIDKDDVHVETKDVDGNTSFENEQTSYLTSVFEK
USA300_FPR3757 DSDISYVAQFLNTKSTFQETHDKSSNMIDKDDVHVETKDVDGNTSFENEQTSYLTSVFEK
************************************************************
COL HLSDEKSEQLLHHMRSGLTLLDSVKIVSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQ
N315 HLSDEKSEQLLHHMRSGLTLLDSVKIVSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQ
NCTC8325 HLSDEKSEQLLHHMRSGLTLLDSVKIVSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQ
Newman HLSDEKSEQLLHHMRSGLTLLDSVKIVSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQ
USA300_FPR3757 HLSDEKSEQLLHHMRSGLTLLDSVKIVSTEVKQWQTYIADYLYQCDVINDPTSFAELLEQ
************************************************************
COL RLIDNPGWILSPYPVAIPHLRDNMIKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFIS
N315 RLIDNPGWILSPYPVAIPHLRDNMIKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFIP
NCTC8325 RLIDNPGWILSPYPVAIPHLRDNMIKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFIS
Newman RLIDNPGWILSPYPVAIPHLRDNMIKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFIS
USA300_FPR3757 RLIDNPGWILSPYPVAIPHLRDNMIKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFIS
***********************************************************.
COL DNDFMASLVSDLSEFLSLKLESIDTFMENPQELETLLRNKFLERIKKQFI
N315 DNDFMASLVSDLSEFLSLKLESIDTFMENPQELETLLRNKFLERIKKQFI
NCTC8325 DNDFMASLVSDLSEFLSLKLESIDTFMENPQELETLLRNKFLERIKKQFI
Newman DNDFMASLVSDLSEFLSLKLESIDTFMENPQELETLLRNKFLERIKKQFI
USA300_FPR3757 DNDFMASLVSDLSEFLSLKLESIDTFMENPQELETLLRNKFLERIKKQFI
**************************************************
- ↑ S A Henstra, M Tuinhof, R H Duurkens, G T Robillard
The Bacillus stearothermophilus mannitol regulator, MtlR, of the phosphotransferase system. A DNA-binding protein, regulated by HPr and iicbmtl-dependent phosphorylation.
J Biol Chem: 1999, 274(8);4754-63
[PubMed:9988713] [WorldCat.org] [DOI] (P p)Thanh Nguyen, Truc Kim, Hai Minh Ta, Won Sik Yeo, Jongkeun Choi, Pushpak Mizar, Seung Seo Lee, Taeok Bae, Akhilesh Kumar Chaurasia, Kyeong Kyu Kim
Targeting Mannitol Metabolism as an Alternative Antimicrobial Strategy Based on the Structure-Function Study of Mannitol-1-Phosphate Dehydrogenase in Staphylococcus aureus.
mBio: 2019, 10(4);
[PubMed:31289190] [WorldCat.org] [DOI] (I e)