⊟Summary[edit | edit source]
- pan ID?: SAUPAN003922000
- symbol?: —
- synonym:
- description?: IDEAL domain-containing protein
- IDEAL domain-containing protein
- putative staphylococcal protein
- YpiB family protein
- domain family
- IDEAL domain protein
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 4242942..4243521
- synteny block?: BlockID0029680
- occurrence?: in 100% of 34 strains
reoY : ReoM-binding ClpCP substrate-recognition adaptor protein [1]
The ReoMY system regulates ClpCP-mediated degradation of MurA, the enzyme catalyzing the first committed step of peptidoglycan biosynthesis. The Stk1/Stp1 serine-threonine kinase/phosphatase system controls the phosphorylation state of ReoM ; dephosphorylated ReoM binds to both MurA and ReoY. ReoY is a ClpCP-binding adaptor protein that targets ReoM's MurA cargo for proteolysis when ReoM is in the dephosphorylated state. When PG synthesis is required, Stk1 phosphorylates ReoM, MurA no longer binds ReoY, MurA is no longer targeted for proteolysis and the rate of cell wall synthesis increases. The alternative UDP-NAG carboxyvinyltransferase MurZ also plays a currently undefined role in targeting MurA for ClpCP-mediated proteolysis.
⊟Orthologs[edit | edit source]
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MSETFNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANIHFVDTKINHHETLEIA
N315 MSETLNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANIHFVDTKINHHETLEIA
NCTC8325 MSETLNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANIHFVDTKINHHETLEIA
Newman MSETFNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANIHFVDTKINHHETLEIA
USA300_FPR3757 MSETFNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANIHFVDTKINHHETLEIA
****:*******************************************************
COL EVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFANVSKREQRLDDLIVAQLT
N315 EVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFANVSKREQRLDDLIVAQLT
NCTC8325 EVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFANVSKREQRLDDLIVAQLT
Newman EVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFANVSKREQRLDDLIVAQLT
USA300_FPR3757 EVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFANVSKREQRLDDLIVAQLT
************************************************************
COL ESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMNEKQRFYQLTQILNTLKLVNK
N315 ESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMNEKQRFYQLTQILNTLKLVNK
NCTC8325 ESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMNEKQRFYQLTQILNTLKLVNK
Newman ESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMNEKQRFYQLTQILNTLKLVNK
USA300_FPR3757 ESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMNEKQRFYQLTQILNTLKLVNK
************************************************************
COL HNQFEDLADDN
N315 HNQFEDLADDD
NCTC8325 HNQFEDLADDN
Newman HNQFEDLADDN
USA300_FPR3757 HNQFEDLADDN
**********:
- ↑ Jessica L Kelliher, Caroline M Grunenwald, Rhiannon R Abrahams, McKenzie E Daanen, Cassandra I Lew, Warren E Rose, John-Demian Sauer
PASTA kinase-dependent control of peptidoglycan synthesis via ReoM is required for cell wall stress responses, cytosolic survival, and virulence in Listeria monocytogenes.
PLoS Pathog: 2021, 17(10);e1009881
[PubMed:34624065] [WorldCat.org] [DOI] (I e)Sabrina Wamp, Patricia Rothe, Daniel Stern, Gudrun Holland, Janina Döhling, Sven Halbedel
MurA escape mutations uncouple peptidoglycan biosynthesis from PrkA signaling.
PLoS Pathog: 2022, 18(3);e1010406
[PubMed:35294506] [WorldCat.org] [DOI] (I e)Malcolm E Winkler, Merrin Joseph, Ho-Ching T Tsui
Secrets of getting started: Regulation of the first committed step of peptidoglycan synthesis by protein phosphorylation in Enterococcus and other Gram-positive bacteria.
Mol Microbiol: 2023, 120(6);805-810
[PubMed:38012814] [WorldCat.org] [DOI] (I p)