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⊟Summary[edit | edit source]
- pan ID?: SAUPAN003552000
- symbol?: hslU
- synonym: clpY
- description?: ATP-dependent protease ATP-binding subunit HslU
- ATP-dependent protease ATP-binding subunit HslU
- HslU--HslV peptidase ATPase subunit
- ATP-dependent protease ATPase subunit HslU
- ATP-dependent hsl protease ATP-binding subunit HslU
- putative ATP-dependent protease ATP-bindingsubunit
- ATP-dependent carboxylate-amine ligase
- ATP-dependent endopeptidase hsl ATP-binding subunit
- ATP-dependent protease ATP-binding subunit of heat shock protein
- heat shock protein HslVU, ATPase subunit HslU
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 3888423..3889835
- synteny block?: BlockID0026290
- occurrence?: in 100% of 34 strains
hslU (clpY) : hslUV proteolytic system chaperone and ATP-binding subunit HslU [1]
• A crystal structure is available : 6KWW
The HslUV proteolytic system is not as well substrate-characterized as other Clp proteases in staphylococci. In the presence of HslU and ATP, HslV is processed to generate a new N-terminus at with Thr-9 as the new catalytic N-terminal residue of a threonine peptidase. HslV oligomerizes as two rings of hexamers capped on either end by a ring of HslU hexamers. HslU then selectively unwinds substrate proteins and feeds them into the HslV proteolytic machine for degradation. The system is mildly induced under heat shock conditions but doesn't have a clear mutant phenotype.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1212 (clpY)
08BA02176:
C248_1285
11819-97:
MS7_1212 (hslU)
6850:
RSAU_001136 (hslU)
71193:
ST398NM01_1256
ECT-R 2:
ECTR2_1110 (hslU)
ED133:
SAOV_1256
ED98:
SAAV_1228 (hslU)
HO 5096 0412:
SAEMRSA15_10870
JH1:
SaurJH1_1339 (hslU)
JH9:
SaurJH9_1314 (hslU)
JKD6008:
SAA6008_01209 (hslU)
JKD6159:
SAA6159_01109 (hslU)
JSNZ:
JSNZ_001224 (hslU)
LGA251:
SARLGA251_11650 (HslU)
M013:
M013TW_1194
MRSA252:
SAR1230 (hslU)
MSHR1132:
SAMSHR1132_10970
MSSA476:
SAS1188 (hslU)
Mu3:
SAHV_1244 (hslU)
Mu50:
SAV1254 (hslU)
MW2:
MW1137 (hslU)
RF122:
SAB1116 (hslU)
ST398:
SAPIG1256 (hslU)
T0131:
SAT0131_01305
TCH60:
HMPREF0772_11976 (hslU)
TW20:
SATW20_12480 (HslU)
USA300_TCH1516:
USA300HOU_1185 (hslU)
VC40:
SAVC_05435 (hslU)
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLLDEESKQEISPKNILMI
N315 MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLLDEESKQEISPKNILMI
NCTC8325 MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLLDEESKQEISPKNILMI
Newman MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLLDEESKQEISPKNILMI
USA300_FPR3757 MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLLDEESKQEISPKNILMI
************************************************************
COL GPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVESMVRDLVDVSVRLVKAQKK
N315 GPTGVGKTEIARRMAKVVGAPFIKVEATKFTELGYVGRDVESMVRDLVDVSVRLVKAQKK
NCTC8325 GPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVESMVRDLVDVSVRLVKAQKK
Newman GPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVESMVRDLVDVSVRLVKAQKK
USA300_FPR3757 GPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVESMVRDLVDVSVRLVKAQKK
********************************:***************************
COL SLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGGAIPNFGQNNEDEEEPPTEEI
N315 SLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGGAIPNFGQNNEDEEEPPTEEI
NCTC8325 SLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGGAIPNFGQNNEDEEEPPTEEI
Newman SLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGGAIPNFGQNNEDEEEPPTEEI
USA300_FPR3757 SLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGGAIPNFGQNNEDEEEPPTEEI
************************************************************
COL KTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTNQNQQMQEMMNQLMPKKKVEREV
N315 KTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTNQNQQMQEMMNQLMPKKKVEREV
NCTC8325 KTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTNQNQQMQEMMNQLMPKKKVEREV
Newman KTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTNQNQQMQEMMNQLMPKKKVEREV
USA300_FPR3757 KTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTNQNQQMQEMMNQLMPKKKVEREV
************************************************************
COL AVETARKILADSYADELIDQESANQEALELAEQMGIIFIDEIDKVATNNHNSGQDVSRQG
N315 AVETARKILADSYADELIDQESANQEALELAEQMGIIFIDEIDKVATNNHNSGQDVSRQG
NCTC8325 AVETARKILADSYADELIDQESANQEALELAEQMGIIFIDEIDKVATNNHNSGQDVSRQG
Newman AVETARKILADSYADELIDQESANQEALELAEQMGIIFIDEIDKVATNNHNSGQDVSRQG
USA300_FPR3757 AVETARKILADSYADELIDQESANQEALELAEQMGIIFIDEIDKVATNNHNSGQDVSRQG
************************************************************
COL VQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAFHVSKPSDLIPELQGRFPIRVELDSLS
N315 VQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAFHVSKPSDLIPELQGRFPIRVELDSLS
NCTC8325 VQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAFHVSKPSDLIPELQGRFPIRVELDSLS
Newman VQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAFHVSKPSDLIPELQGRFPIRVELDSLS
USA300_FPR3757 VQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAFHVSKPSDLIPELQGRFPIRVELDSLS
************************************************************
COL VEDFVRILTEPKLSLIKQYEALLQTEEVTVNFTDEAITRLAEIAYQVNQDTDNIGARRLH
N315 VEDFVRILTEPKLSLIKQYEALLQTEEVTVNFTDEAITRLAEIAYQVNQDTDNIGARRLH
NCTC8325 VEDFVRILTEPKLSLIKQYEALLQTEEVTVNFTDEAITRLAEIAYQVNQDTDNIGARRLH
Newman VEDFVRILTEPKLSLIKQYEALLQTEEVTVNFTDEAITRLAEIAYQVNQDTDNIGARRLH
USA300_FPR3757 VEDFVRILTEPKLSLIKQYEALLQTEEVTVNFTDEAITRLAEIAYQVNQDTDNIGARRLH
************************************************************
COL TILEKMLEDLSFEAPSMPNAVVDITPQYVDDKLKSISTNKDLSAFIL
N315 TILEKMLEDLSFEAPSMPNAVVDITPQYVDDKLKSISTNKDLSAFIL
NCTC8325 TILEKMLEDLSFEAPSMPNAVVDITPQYVDDKLKSISTNKDLSAFIL
Newman TILEKMLEDLSFEAPSMPNAVVDITPQYVDDKLKSISTNKDLSAFIL
USA300_FPR3757 TILEKMLEDLSFEAPSMPNAVVDITPQYVDDKLKSISTNKDLSAFIL
***********************************************
- ↑ Dorte Frees, Line E Thomsen, Hanne Ingmer
Staphylococcus aureus ClpYQ plays a minor role in stress survival.
Arch Microbiol: 2005, 183(4);286-91
[PubMed:15843987] [WorldCat.org] [DOI] (P p)Soyeon Jeong, Jinsook Ahn, Ae-Ran Kwon, Nam-Chul Ha
Cleavage-Dependent Activation of ATP-Dependent Protease HslUV from Staphylococcus aureus.
Mol Cells: 2020, 43(8);694-704
[PubMed:32694241] [WorldCat.org] [DOI] (I p)