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⊟Summary[edit | edit source]
- pan ID?: SAUPAN003529000
- symbol?: trmD
- synonym:
- description?: tRNA (guanosine(37)-N1)-methyltransferase TrmD
- tRNA (guanosine(37)-N1)-methyltransferase TrmD
- tRNA (guanine-N(1)-)-methyltransferase
- tRNA (Guanine-1)-methyltransferase
- putative tRNA (guanine-7-)-methyltransferase
- tRNA (Guanine37-N1) -methyltransferase
- tRNA (guanine-N1)-methyltransferase
- tRNA guanine-N(1)-methyltransferase
- tRNA methyltransferase
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 3868267..3869004
- synteny block?: BlockID0026070
- occurrence?: in 97% of 34 strains
trmD : tRNA guanosine(37) N-(1)-methyltransferase [1]
• A crystal structure is available : 3KY7
Several transfer RNAs require modifications to provide maximal fidelity during protein synthesis. TrmD is a methyltransferase specific for guanosines at tRNA position 37 generating 1-methylguanosine ("m1G" or "K"). Staphylococcal tRNAs requiring this modification include: tRNAH1GUG, tRNAH2GUG, tRNAPUGG, tRNAR3ACG, tRNAL3UAG.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_1198 (trmD)
08BA02176:
C248_1271 (trmD)
11819-97:
MS7_1198 (trmD)
6850:
RSAU_001122 (trmD)
71193:
ST398NM01_1240
ECT-R 2:
ECTR2_1096 (trmD)
ED133:
SAOV_1243 (trmD)
ED98:
SAAV_1213 (trmD)
HO 5096 0412:
SAEMRSA15_10730 (trmD)
JH1:
SaurJH1_1324 (trmD)
JH9:
SaurJH9_1299 (trmD)
JKD6008:
SAA6008_01195 (trmD)
JKD6159:
SAA6159_01093 (trmD)
JSNZ:
JSNZ_001209 (trmD)
LGA251:
SARLGA251_11510 (trmD)
M013:
M013TW_1178
MRSA252:
SAR1216 (trmD)
MSHR1132:
SAMSHR1132_10830
MSSA476:
SAS1174 (trmD)
Mu3:
SAHV_1230 (trmD)
Mu50:
SAV1240 (trmD)
MW2:
MW1123 (trmD)
RF122:
SAB1104 (trmD)
ST398:
SAPIG1240 (trmD)
T0131:
SAT0131_01290
TCH60:
HMPREF0772_11991 (trmD)
TW20:
SATW20_12340 (trmD)
USA300_TCH1516:
—
VC40:
SAVC_05365 (trmD)
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAINKHNQVDDYPYGGGQGM
N315 MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAINKHNQVDDYPYGGGQGM
NCTC8325 MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAINKHNQVDDYPYGGGQGM
Newman MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAINKHNQVDDYPYGGGQGM
USA300_FPR3757 MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAINKHNQVDDYPYGGGQGM
************************************************************
COL VLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSKADHIVFICGHYEGYDERI
N315 VLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSKADHIVFICGHYEGYDERI
NCTC8325 VLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSKADHIVFICGHYEGYDERI
Newman VLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSKADHIVFICGHYEGYDERI
USA300_FPR3757 VLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSKADHIVFICGHYEGYDERI
************************************************************
COL RTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGNEQSHQDDSFSDGLLEFPQYT
N315 RTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGNEQSHQDDSFSDGLLEFPQYT
NCTC8325 RTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGNEQSHQDDSFSDGLLEFPQYT
Newman RTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGNEQSHQDDSFSDGLLEFPQYT
USA300_FPR3757 RTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGNEQSHQDDSFSDGLLEFPQYT
************************************************************
COL RPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKRPDLIEKYPLTNADKQILERYKI
N315 RPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKRPDLIEKYPLTNEDKQILERYKI
NCTC8325 RPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKRPDLIEKYPLTNADKQILERYKI
Newman RPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKRPDLIEKYPLTNADKQILERYKI
USA300_FPR3757 RPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKRPDLIEKYPLTNADKQILERYKI
************************************************* **********
COL GLKKG
N315 GLKKG
NCTC8325 GLKKG
Newman GLKKG
USA300_FPR3757 GLKKG
*****
- ↑ Yong Zhang, Rym Agrebi, Lauren E Bellows, Jean-François Collet, Volkhard Kaever, Angelika Gründling
Evolutionary Adaptation of the Essential tRNA Methyltransferase TrmD to the Signaling Molecule 3',5'-cAMP in Bacteria.
J Biol Chem: 2017, 292(1);313-327
[PubMed:27881678] [WorldCat.org] [DOI] (I p)Laura Antoine, Philippe Wolff, Eric Westhof, Pascale Romby, Stefano Marzi
Mapping post-transcriptional modifications in Staphylococcus aureus tRNAs by nanoLC/MSMS.
Biochimie: 2019, 164;60-69
[PubMed:31295507] [WorldCat.org] [DOI] (I p)