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⊟Summary[edit | edit source]
- pan ID?: SAUPAN002596000
- symbol?: queE
- synonym:
- description?: 7-carboxy-7-deazaguanine synthase QueE
- 7-carboxy-7-deazaguanine synthase QueE
- radical activating enzyme family protein
- 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein QueE
- 7-cyano-7-deazaguanosine biosynthesis protein, putative
- 7-cyano-7-deazaguanosine (preQ0) biosynthesis protein
- 7-cyano-7-deazaguanosine biosynthesis protein QueE
- coenzyme PQQ synthesis homologue
- coenzyme PQQ synthesis-like protein
- organic radical-activating protein
- putative radical activating enzyme
- Queuosine Biosynthesis QueE Radical SAM
- queuosine biosynthesis radical SAM protein QueE
- radical activating enzyme
- radical SAM domain-containing protein
- radical SAM protein
descriptions from strain specific annotations:
- strand?: -
- coordinates?: 3107136..3107849
- synteny block?: BlockID0018320
- occurrence?: in 100% of 34 strains
queE : 7-carboxy-7-deazaguanine synthase [1]
QueE is the second committed step in the de novo synthesis of the heavily-modified nucleobase queuosine. Its substrate, 6-carboxy-5,6,7,8-tetrahydropterin (CPH4), is provided by the carboxyltransferase QueD. QueE performs a series of enzymatically-complex steps on CPH4 involving radical intermediates, ring opening, deamination and ring closure, ultimately resulting in 7-carboxy-7-deazaguanine.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_0687 (queE)
08BA02176:
C248_0794
11819-97:
MS7_0761 (queE)
6850:
RSAU_000685 (queE)
71193:
ST398NM01_0787
ECT-R 2:
ECTR2_661 (queE)
ED133:
SAOV_0744c
ED98:
SAAV_0672
HO 5096 0412:
SAEMRSA15_06360
JH1:
SaurJH1_0750
JH9:
SaurJH9_0734
JKD6008:
SAA6008_00726 (queE)
JKD6159:
SAA6159_00665 (queE)
JSNZ:
JSNZ_000678 (queE)
LGA251:
SARLGA251_06430
M013:
M013TW_0696
MRSA252:
SAR0763
MSHR1132:
SAMSHR1132_06550
MSSA476:
SAS0675
Mu3:
SAHV_0707
Mu50:
SAV0710
MW2:
MW0672
RF122:
SAB0659c
ST398:
SAPIG0787 (queE)
T0131:
SAT0131_00780 (ldh1)
TCH60:
HMPREF0772_12477 (queE)
TW20:
SATW20_07850
USA300_TCH1516:
USA300HOU_0733 (exsD)
VC40:
SAVC_03185
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAFTWDGSAKGDIKLMTAE
N315 MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAFTWDGSAKGDIKLMTAE
NCTC8325 MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAFTWDGSAKGDIKLMTAE
Newman MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAFTWDGSAKGDIKLMTAE
USA300_FPR3757 MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAFTWDGSAKGDIKLMTAE
************************************************************
COL EIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGIFSALETQGSKFQPWMTQI
N315 EIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGIFSALETQGSKFQPWMTQI
NCTC8325 EIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGIFSALETQGSKFQPWMTQI
Newman EIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGIFSALETQGSKFQPWMTQI
USA300_FPR3757 EIYDELKRIGGDLFNHVTISGGNPALIKGIQELVDLFQDKGIFSALETQGSKFQPWMTQI
************************************************************
COL DDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVFDDKDYDFAKMIHHRYPDIPF
N315 DDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVFDDKDYDFAKMIHHRYPDIPF
NCTC8325 DDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVFDDKDYDFAKMIHHRYPDIPF
Newman DDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVFDDKDYDFAKMIHHRYPDIPF
USA300_FPR3757 DDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVFDDKDYDFAKMIHHRYPDIPF
************************************************************
COL YLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMNHVYVLPQLHTLLWSNKKGV
N315 YLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMNHVYVLPQLHTLLWSNKKGV
NCTC8325 YLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMNHVYVLPQLHTLLWSNKKGV
Newman YLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMNHVYVLPQLHTLLWSNKKGV
USA300_FPR3757 YLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMNHVYVLPQLHTLLWSNKKGV
*********************************************************
- ↑ Geoffrey Hutinet, Manal A Swarjo, Valérie de Crécy-Lagard
Deazaguanine derivatives, examples of crosstalk between RNA and DNA modification pathways.
RNA Biol: 2017, 14(9);1175-1184
[PubMed:27937735] [WorldCat.org] [DOI] (I p)Laura Antoine, Philippe Wolff, Eric Westhof, Pascale Romby, Stefano Marzi
Mapping post-transcriptional modifications in Staphylococcus aureus tRNAs by nanoLC/MSMS.
Biochimie: 2019, 164;60-69
[PubMed:31295507] [WorldCat.org] [DOI] (I p)Jorge Díaz-Rullo, José Eduardo González-Pastor
tRNA queuosine modification is involved in biofilm formation and virulence in bacteria.
Nucleic Acids Res: 2023, 51(18);9821-9837
[PubMed:37638766] [WorldCat.org] [DOI] (I p)Valérie de Crécy-Lagard, Geoffrey Hutinet, José D D Cediel-Becerra, Yifeng Yuan, Rémi Zallot, Marc G Chevrette, R M Madhushi N Ratnayake, Marshall Jaroch, Samia Quaiyum, Steven Bruner
Biosynthesis and function of 7-deazaguanine derivatives in bacteria and phages.
Microbiol Mol Biol Rev: 2024, 88(1);e0019923
[PubMed:38421302] [WorldCat.org] [DOI] (I p)