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⊟Summary[edit | edit source]

  • pan ID?: SAUPAN002122000
  • symbol?: hsdS1
  • synonym:
  • description?: restriction endonuclease subunit S

      descriptions from strain specific annotations:

    • restriction endonuclease subunit S
    • restriction modification system specificity subunit
    • putative restriction/modification system specificity protein
    • type I restriction modification system, site specificity determination subunit
    • type I restriction-modification system, S subunit
    • type I restriction-modification system S subunit
    • EcoA family type I restriction-modification system
    • restriction and modification system specificity protein
    • restriction endonuclease S subunit
    • Restriction modification system DNA specificity domain protein
    • restriction modification system DNA specificity subunit
    • type I restriction modification DNA specificity domain protein
    • Type I restriction-modification system, specificity subunit S
    • type I restriction-modification system specificity protein
    • type I restriction-modification system subunit S
    • type I site-specific deoxyribonuclease specificity subunit
  • strand?: +
  • coordinates?: 2613264..2615281
  • synteny block?: BlockID0014280
  • occurrence?: in 62% of 34 strains

hsdS : type I restriction-modification system specificity subunit [1]

Staphylococci restrict horizontal gene transfer even within the same species with lineage-specific combinations of HsdS and HsdM protein pairs. HsdS recognizes specific sequences lacking a methylation mark that are subsequently either (1) methylated by HsdM if the DNA is already hemi-methylated (self) or (2) restricted by HsdR if the DNA is non-methylated on both strands. This provides significant protection to the bacterium against bacteriophage predation from non-matched hosts.

HsdS Specificity by Clonal Complex

Clonal Complex hsdS Genes Present Orthologs Specificities (if known) Notes
1 hsdS1-1, hsdS1-2 MW2: MW0393, MW1750 hsdS1-1: CCAY(N)5TTAA

hsdS1-2: CCAY(N)6TGT

hsdS1-2 has the same recognition sequence as hsdS8-2.
5 hsdS5-1, hsdS5-2 hsdS5-1: AGG(N)5GAT

hsdS5-2: CCAY(N)6GTA

8 hsdS8-1, hsdS8-2 NCTC8325: SAOUHSC_00398,

USA300_FPR3757: SAUSA300_0406, SAUSA300_1751

USA300_TCH1516: USA300HOU_0434,

hsdS8-1: AGG(N)5GAT

hsdS8-2: CCAY(N)6TGT

22 hsdS22-1 hsdS22-1: AGG(N)6TGAR
30 hsdS30-1, hsdS30-2 hsdS30-1: GWAG(N)5GAT

hsdS30-2: GGA(N)7TCG

45 hsdS45-1 hsdS45-1: GWAG(N)6TAAA
93 hsdS93-1, hsdS93-2, hsds93-7 hsdS93-1: GAAG(N)5TAC

hsdS93-2: GGHA(N)7TCG hsdS93-3: CAG(N)6TTC

97 hsdS97-1 hsdS97-1: CCAY(N)6RTC

⊟Orthologs[edit | edit source]

    COL:
    SACOL0477
    N315:
    SA0392 (hsdS)
    NCTC8325:
    SAOUHSC_00398
    Newman:
    NWMN_0399 (hsdS)
    USA300_FPR3757:
    SAUSA300_0406
    JSNZ:
    EGJ38_000367 (hsdS1)
    04-02981:
    SA2981_0408 (hsdS)
    08BA02176:
    —
    11819-97:
    —
    6850:
    RSAU_000361
    71193:
    —
    ECT-R 2:
    ECTR2_367
    ED133:
    —
    ED98:
    SAAV_0375 (hsdS1)
    HO 5096 0412:
    —
    JH1:
    SaurJH1_0467
    JH9:
    SaurJH9_0455
    JKD6008:
    SAA6008_00435 (hsdS)
    JKD6159:
    —
    LGA251:
    —
    M013:
    —
    MRSA252:
    SAR0434
    MSHR1132:
    —
    MSSA476:
    —
    Mu3:
    SAHV_0430 (hsdS)
    Mu50:
    SAV0432 (hsdS)
    MW2:
    —
    RF122:
    —
    ST398:
    —
    T0131:
    SAT0131_00471
    TCH60:
    HMPREF0772_10085
    TW20:
    SATW20_05090
    USA300_TCH1516:
    USA300HOU_0434 (hsdS1)
    VC40:
    SAVC_01790

⊟Genome Viewer[edit | edit source]

COL
N315
NCTC8325
Newman
USA300_FPR3757
JSNZ

⊟Alignments[edit | edit source]

  • alignment of orthologues:
    CLUSTAL format alignment by MAFFT L-INS-i (v7.505)


    COL             MSNTQKKNVPELRFPGFEGEWEEKQLGDLTDRVIRKNKNLESKKPLTISGQLGLIDQTEY
    N315            MSNTQKKNVPELRFPGFEGEWEEKQLGDLTDRVIRKNKNLESKKPLTISGQLGLIDQTEY
    NCTC8325        MSNTQKKNVPELRFPGFEGEWEEKQLGDLTDRVIRKNKNLESKKPLTISGQLGLIDQTEY
    Newman          MSNTQKKNVPELRFPGFEGEWEEKQLGDLTDRVIRKNKNLESKKPLTISGQLGLIDQTEY
    USA300_FPR3757  MSNTQKKNVPELRFPGFEGEWEEKQLGDLTDRVIRKNKNLESKKPLTISGQLGLIDQTEY
    JSNZ            MSNTQTKNVPELKFPEFEGEWEEKKLGEFAGKVTKKNVDKKYIETLTNSAELGIISQKDY
                    *****.******:** ********:**:::.:* :** : :  :.** *.:**:*.*.:*

    COL             FSKSVSS-KNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRYDSGVLSSLYICFSIKSE
    N315            FSKSVSS-KNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRYDSGVLSSLYICFSIKSE
    NCTC8325        FSKSVSS-KNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRYDSGVLSSLYICFSIKSE
    Newman          FSKSVSS-KNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRYDSGVLSSLYICFSIKSE
    USA300_FPR3757  FSKSVSS-KNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRYDSGVLSSLYICFSIKSE
    JSNZ            FDKEISNIDNIKKYYVVEENDFVYNPRISNYAPFGPVNRNKLGKKGVMSPLYTVFKIQN-
                    *.*.:*. .*:::* ::::.:*.**   **  *:*.::* .  ..**:*.**  *.*:. 

    COL             MSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFFTILIKYPSLEEQQKIGKFF
    N315            MSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFFTILIKYPSLEEQQKIGKFF
    NCTC8325        MSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFFTILIKYPSLEEQQKIGKFF
    Newman          MSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFFTILIKYPSLEEQQKIGKFF
    USA300_FPR3757  MSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFFTILIKYPSLEEQQKIGKFF
    JSNZ            IDLNFIEFYFKSSKWYRFMALNGDSGARAD-RFSIKNRTFMEMPLHIPCMDEQIKIGQFF
                    :. :*:* **.*::*** ::  . .*** .  :.:. . *: : :: *.::** ***:**

    COL             SKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEDYPDWENSKIEKYLKERNE
    N315            SKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEDYPDWENSKIEKYLKERNE
    NCTC8325        SKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEDYPDWENSKIEKYLKERNE
    Newman          SKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEDYPDWENSKIEKYLKERNE
    USA300_FPR3757  SKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGEDYPDWENSKIEKYLKERNE
    JSNZ            SKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKDENGNDYPEWEERRFADIFKFHNK
                    ****************************************:***:**: :: . :* :*:

    COL             -RSDKGQMLSVTIN------SGIIKFSELDRKDNS----SKDKSNYKVVRKNDIAYNSMR
    N315            -RSDKGQMLSVTIN------SGIIKFSELDRKDNS----SKDKSNYKVVRKNDIAYNSMR
    NCTC8325        -RSDKGQMLSVTIN------SGIIKFSELDRKDNS----SKDKSNYKVVRKNDIAYNSMR
    Newman          -RSDKGQMLSVTIN------SGIIKFSELDRKDNS----SKDKSNYKVVRKNDIAYNSMR
    USA300_FPR3757  -RSDKGQMLSVTIN------SGIIKFSELDRKDNS----SKDKSNYKVVRKNDIAYNSMR
    JSNZ            LRKPIKENLRVKGSYPYYGATGIIDYVDDFIFDGNYLLIGEDGANI-ITRSAPLVY----
                     *.   : * *. .      :***.: :    *..    .:* :*  :.*.  :.*    

    COL             MWQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIHKFKINSQGLTSDTWNL
    N315            MWQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIHKFKINSQGLTSDTWNL
    NCTC8325        MWQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIHKFKINSQGLTSDTWNL
    Newman          MWQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIHKFKINSQGLTSDTWNL
    USA300_FPR3757  MWQGASGKSNYNGIVSPAYTVLYPTQNTSSLFIGYKFKTHRMIHKFKINSQGLTSDTWNL
    JSNZ            LVNGKFWVNNHAHILSPL-----------NGNIQYLYQVAELVNYEKYNT---GTAQPKL
                    : :*    .*:  *:**            .  * * ::. .:::  * *:    :   :*

    COL             KYKQLKNINIDIPV-LEEQEKIGDFFKKMDILISKQKMKIEILEKEKQSFLQKMFL
    N315            KYKQLKNINIDIPV-LEEQEKIGDFFKKMDILISKQKMKIEILEKEKQSFLQKMFL
    NCTC8325        KYKQLKNINIDIPV-LEEQEKIGDFFKKMDILISKQKMKIEILEKEKQSFLQKMFL
    Newman          KYKQLKNINIDIPV-LEEQEKIGDFFKKMDILISKQKMKIEILEKEKQSFLQKMFL
    USA300_FPR3757  KYKQLKNINIDIPV-LEEQEKIGDFFKKMDILISKQKMKIEILEKEKQSFLQKMFL
    JSNZ            NIQNLKIISVVISTNLEEQQKIGSFLSKLDRQIDLEEQKLELLQQRKKALLKSMFV
                    : ::** *.: *.. ****:***.*:.*:*  *. :: *:*:*::.*:::*:.**:

  1. ↑ Denise E Waldron, Jodi A Lindsay
    Sau1: a novel lineage-specific type I restriction-modification system that blocks horizontal gene transfer into Staphylococcus aureus and between S. aureus isolates of different lineages.
    J Bacteriol: 2006, 188(15);5578-85
    [PubMed:16855248] [WorldCat.org] [DOI] (P p)    Ian R Monk, Timothy J Foster
    Genetic manipulation of Staphylococci-breaking through the barrier.
    Front Cell Infect Microbiol: 2012, 2;49
    [PubMed:22919640] [WorldCat.org] [DOI] (I e)    Ian R Monk, Jai J Tree, Benjamin P Howden, Timothy P Stinear, Timothy J Foster
    Complete Bypass of Restriction Systems for Major Staphylococcus aureus Lineages.
    mBio: 2015, 6(3);e00308-15
    [PubMed:26015493] [WorldCat.org] [DOI] (I e)    Christopher D Johnston, Sean L Cotton, Susan R Rittling, Jacqueline R Starr, Gary G Borisy, Floyd E Dewhirst, Katherine P Lemon
    Systematic evasion of the restriction-modification barrier in bacteria.
    Proc Natl Acad Sci U S A: 2019, 116(23);11454-11459
    [PubMed:31097593] [WorldCat.org] [DOI] (I p)
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