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⊟Summary[edit | edit source]
- pan ID?: SAUPAN000024000
- symbol?: gdpP
- synonym:
- description?: DHH family phosphoesterase
- DHH family phosphoesterase
- cyclic-di-AMP phosphodiesterase GdpP
- DHH family phosphoesterase, putative
- phosphoesterase, DHH family protein
- DHHA1 domain protein
- DHH family protein
- DHH subfamily 1 protein
- DHH subfamily phosphodiesterase
- phosphoesterase domain-containing protein
- putative bifunctional signaling protein/50S ribosomal protein L9
descriptions from strain specific annotations:
- strand?: +
- coordinates?: 24265..26250
- synteny block?: BlockID0000210
- occurrence?: in 100% of 34 strains
gdpP : cyclic-di-AMP phosphodiesterase [1]
• A crystal structure is available : 5XSI
Bacteria need to carefully balance their intracellular osmolyte concentration with peptidoglycan synthesis to ensure the cell's structural integrity can tolerate the cell's turgor pressure. Staphylococci do this using the second messenger nucleotide cyclic-di-AMP which positively regulates osmolyte uptake coupled with cell wall synthesis through the dacA-cdaR-glmM operon. DacA is a diadenylate cyclase which generates the nucleotide second messenger signal cyclic-di-AMP whereas GdpP is a phosphodiesterase that degrades the second messenger nucleotide. If GdpP function is disrupted, bacteria become more resilient to beta lactam exposure. Whether this is due to cell wall thickening due to lipoteichoic acid modification, enhanced phosphoglucosamine mutase activity or an indirect effect of cyclic-di-AMP amplification is unclear.
⊟Orthologs[edit | edit source]
04-02981:
SA2981_0014
08BA02176:
C248_0014
11819-97:
MS7_0014
6850:
RSAU_000014
71193:
ST398NM01_0014
ECT-R 2:
ECTR2_14
ED133:
SAOV_0014
ED98:
SAAV_0016
HO 5096 0412:
SAEMRSA15_00140
JH1:
SaurJH1_0014
JH9:
SaurJH9_0014
JKD6008:
SAA6008_00014
JKD6159:
SAA6159_00014
JSNZ:
JSNZ_000014 (gdpP)
LGA251:
SARLGA251_00140
M013:
M013TW_0015
MRSA252:
SAR0014
MSHR1132:
SAMSHR1132_00140
MSSA476:
SAS0014
Mu3:
SAHV_0014
Mu50:
SAV0014
MW2:
MW0014
RF122:
SAB0014
ST398:
SAPIG0014
T0131:
SAT0131_00016
TCH60:
HMPREF0772_10454
TW20:
SATW20_00140
USA300_TCH1516:
USA300HOU_0014
VC40:
SAVC_00070
⊟Genome Viewer[edit | edit source]
| COL | |
| N315 | |
| NCTC8325 | |
| Newman | |
| USA300_FPR3757 |
⊟Alignments[edit | edit source]
- alignment of orthologues: CLUSTAL format alignment by MAFFT L-INS-i (v7.307)
COL MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLVVMIIVSIILFRQALMK
N315 MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLVVMIIVSIILFRQALMK
NCTC8325 MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLVVMIIVSIILFRQALMK
Newman MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLVVMIIVSIILFRQALMK
USA300_FPR3757 MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLVVMIIVSIILFRQALMK
************************************************************
COL MDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQFMTDHMEANVISESVNEVF
N315 MDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQFMTNHMEANVISESVNEVF
NCTC8325 MDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQFMTDHMEANVISESVNEVF
Newman MDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQFMTDHMEANVISESVNEVF
USA300_FPR3757 MDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQFMTDHMEANVISESVNEVF
********************************************:***************
COL PNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITEQVQTNELYENSKPIIATLFL
N315 PNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITEQVQTNELYENSKPIIATLFL
NCTC8325 PNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITEQVQTNELYENSKPIIATLFL
Newman PNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITEQVQTNELYENSKPIIATLFL
USA300_FPR3757 PNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITEQVQTNELYENSKPIIATLFL
************************************************************
COL DNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFKRYSSDQFVAYLNQKILADLEES
N315 DNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFKRYSSDQFVAYLNQKILADLEES
NCTC8325 DNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFKRYSSDQFVAYLNQKILADLEES
Newman DNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFKRYSSDQFVAYLNQKILADLEES
USA300_FPR3757 DNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFKRYSSDQFVAYLNQKILADLEES
************************************************************
COL KFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLGELSQSGLDLALGRGGDQVAIKSIN
N315 KFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLGELSQSGLDLALGRGGDQVAIKSIN
NCTC8325 KFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLGELSQSGLDLALGRGGDQVAIKSIN
Newman KFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLGELSQSGLDLALGRGGDQVAIKSIN
USA300_FPR3757 KFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLGELSQSGLDLALGRGGDQVAIKSIN
************************************************************
COL GNVRFYGGKTDPMEKRTRVRARVISHALKDILAEGDKVIIMGHKRPDLDAIGAAIGVSRF
N315 GNVRFYGGKTDPMEKRTRVRARVISHALKDILAEGDKVIIMGHKRPDLDAIGAAIGVSRF
NCTC8325 GNVRFYGGKTDPMEKRTRVRARVISHALKDILAEGDKVIIMGHKRPDLDAIGAAIGVSRF
Newman GNVRFYGGKTDPMEKRTRVRARVISHALKDILAEGDKVIIMGHKRPDLDAIGAAIGVSRF
USA300_FPR3757 GNVRFYGGKTDPMEKRTRVRARVISHALKDILAEGDKVIIMGHKRPDLDAIGAAIGVSRF
************************************************************
COL AMMNNLEAYIVLNETDIDPTLRRVMNEIDKKPELRERFITSDDAWDMMTSKTTVVIVDTH
N315 AMMNNLEAYIVLNETDIDPTLRRVMNEIDKKSELRERFITSDDAWDMMTSKTTVVIVDTH
NCTC8325 AMMNNLEAYIVLNETDIDPTLRRVMNEIDKKPELRERFITSDDAWDMMTSKTTVVIVDTH
Newman AMMNNLEAYIVLNETDIDPTLRRVMNEIDKKPELRERFITSDDAWDMMTSKTTVVIVDTH
USA300_FPR3757 AMMNNLEAYIVLNETDIDPTLRRVMNEIDKKPELRERFITSDDAWDMMTSKTTVVIVDTH
*******************************.****************************
COL KPELVLDENVLNKANRKVVIDHHRRGESFISNPLLIYMEPYASSTAELVTELLEYQPTEQ
N315 KPELVLDENVLNKANRKVVIDHHRRGESFISNPLLIYMEPYASSTAELVTELLEYQPTEQ
NCTC8325 KPELVLDENVLNKANRKVVIDHHRRGESFISNPLLIYMEPYASSTAELVTELLEYQPTEQ
Newman KPELVLDENVLNKANRKVVIDHHRRGESFISNPLLIYMEPYASSTAELVTELLEYQPTEQ
USA300_FPR3757 KPELVLDENVLNKANRKVVIDHHRRGESFISNPLLIYMEPYASSTAELVTELLEYQPTEQ
************************************************************
COL RLTRLESTVMYAGIIVDTRNFTLRTGSRTFDAASYLRAHGADTILTQHFLKDDVDTYINR
N315 RLTRLESTVMYAGIIVDTRNFTLRTGSRTFDAASYLRAHGADTILTQHFLKDDVDTYINR
NCTC8325 RLTRLESTVMYAGIIVDTRNFTLRTGSRTFDAASYLRAHGADTILTQHFLKDDVDTYINR
Newman RLTRLESTVMYAGIIVDTRNFTLRTGSRTFDAASYLRAHGADTILTQHFLKDDVDTYINR
USA300_FPR3757 RLTRLESTVMYAGIIVDTRNFTLRTGSRTFDAASYLRAHGADTILTQHFLKDDVDTYINR
************************************************************
COL SELIRTVKVEDNGIAIAHGSDDKIYHPVTVAQAADELLSLEGIEASYVVARREDNLIGIS
N315 SELIRTVKVEDNGIAIAHGSDDKIYHPVTVAQAADELLSLEGIEASYVVARREDNLIGIS
NCTC8325 SELIRTVKVEDNGIAIAHGSDDKIYHPVTVAQAADELLSLEGIEASYVVARREDNLIGIS
Newman SELIRTVKVEDNGIAIAHGSDDKIYHPVTVAQAADELLSLEGIEASYVVARREDNLIGIS
USA300_FPR3757 SELIRTVKVEDNGIAIAHGSDDKIYHPVTVAQAADELLSLEGIEASYVVARREDNLIGIS
************************************************************
COL ARSLGSVNVQLTMEALGGGGHLTNAATQLKGVTVEEAIAQLQQAITEQLSRSEDA
N315 ARSLGSVNVQLTMEALGGGGHLTNAATQLKGVTVEEAIAQLQQAITEQLSRSEDA
NCTC8325 ARSLGSVNVQLTMEALGGGGHLTNAATQLKGVTVEEAIAQLQQAITEQLSRSEDA
Newman ARSLGSVNVQLTMEALGGGGHLTNAATQLKGVTVEEAIAQLQQAITEQLSRSEDA
USA300_FPR3757 ARSLGSVNVQLTMEALGGGGHLTNAATQLKGVTVEEAIAQLQQAITEQLSRSEDA
*******************************************************
- ↑ Raymond Poon, Li Basuino, Nidhi Satishkumar, Aditi Chatterjee, Nagaraja Mukkayyan, Emma Buggeln, Liusheng Huang, Vinod Nair, Maria A Argudín, Sandip K Datta, Henry F Chambers, Som S Chatterjee
Loss of GdpP Function in Staphylococcus aureus Leads to β-Lactam Tolerance and Enhanced Evolution of β-Lactam Resistance.
Antimicrob Agents Chemother: 2022, 66(2);e0143121
[PubMed:34843389] [WorldCat.org] [DOI] (I p)