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NCBI: 03-AUG-2016
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus NCTC8325
- locus tag: SAOUHSC_01618
- pan locus tag?: SAUPAN004076000
- symbol: SAOUHSC_01618
- pan gene symbol?: ispA
- synonym:
- product: geranyltranstransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
⊟Accession numbers[edit | edit source]
- Gene ID: 3919956 NCBI
- RefSeq: YP_500132 NCBI
- BioCyc: G1I0R-1505 BioCyc
- MicrobesOnline: 1290046 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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841ATGACGAATCTACCGATGAATAAATTAATAGATGAAGTCAATAATGAATTATCGGTTGCG
ATAAATAAATCAGTAATGGATACTCAGCTAGAAGAAAGTATGTTGTATTCATTAAATGCT
GGAGGTAAACGCATCCGACCAGTTCTGTTATTACTCACTTTAGATTCACTAAATACCGAG
TATGAGTTAGGTATGAAGAGCGCAATTGCACTAGAAATGATTCATACATATTCACTTATT
CATGATGACCTACCAGCGATGGATAATGATGATTATCGACGAGGAAAATTAACAAATCAT
AAAGTATATGGTGAGTGGACTGCGATATTAGCAGGTGATGCTTTATTAACTAAAGCATTT
GAACTTATTTCAAGTGATGATAGATTAACTGATGAAGTAAAAATAAAAGTTCTACAACGG
CTGTCAATAGCAAGTGGTCATGTTGGAATGGTCGGCGGTCAAATGTTAGATATGCAAAGC
GAAGGCCAACCAATTGATCTTGAAACTTTGGAAATGATACACAAAACAAAAACAGGAGCA
TTATTAACTTTTGCGGTTATGAGTGCAGCAGATATCGCTAATGTCGATGATACAACTAAA
GAACATTTAGAAAGTTATAGTTATCATTTAGGTATGATGTTCCAGATTAAAGATGATTTA
TTAGACTGCTATGGTGATGAAGCAAAGTTAGGTAAAAAAGTGGGCAGCGATCTTGAAAAT
AATAAAAGTACGTACGTGAGTTTATTAGGGAAAGATGGCGCAGAAGATAAATTGACTTAT
CATAGAGACGCAGCAGTGGATGAACTAACGCAAATTGATGAACAATTCAATACAAAACAC
TTATTAGAAATCGTTGATTTATTTTATAGCAGGGATCACTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SAOUHSC_01618
- symbol: SAOUHSC_01618
- description: geranyltranstransferase
- length: 293
- theoretical pI: 4.49116
- theoretical MW: 32808.1
- GRAVY: -0.272696
⊟Function[edit | edit source]
- reaction: EC 2.5.1.10? ExPASy(2E,6E)-farnesyl diphosphate synthase Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone heptaprenyl diphosphate synthase component II (TIGR02748; EC 2.5.1.30; HMM-score: 119.1)Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone solanesyl diphosphate synthase (TIGR02749; EC 2.5.1.-; HMM-score: 104.5)
- TheSEED :
- (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10)
- Dimethylallyltransferase (EC 2.5.1.1)
- Geranylgeranyl pyrophosphate synthetase (EC 2.5.1.29)
- Octaprenyl diphosphate synthase (EC 2.5.1.90)
Fatty Acids, Lipids, and Isoprenoids Isoprenoids Archaeal lipids (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10)and 5 moreFatty Acids, Lipids, and Isoprenoids Isoprenoids Carotenoids (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10)Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoid Biosynthesis (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10)Fatty Acids, Lipids, and Isoprenoids Isoprenoids Isoprenoid Biosynthesis: Interconversions (2E,6E)-farnesyl diphosphate synthase (EC 2.5.1.10) - PFAM: Terp_synthase (CL0613) polyprenyl_synt; Polyprenyl synthetase (PF00348; HMM-score: 191.1)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 9.97
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0.01
- Extracellular Score: 0.02
- Internal Helices: 0
- DeepLocPro: Cytoplasmic
- Cytoplasmic Score: 0.9914
- Cytoplasmic Membrane Score: 0.0068
- Cell wall & surface Score: 0.0001
- Extracellular Score: 0.0018
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.002222
- TAT(Tat/SPI): 0.000218
- LIPO(Sec/SPII): 0.00037
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKRIRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKVYGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQSEGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDTTKEHLESYSYHLGMMFQIKDDLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVDELTQIDEQFNTKHLLEIVDLFYSRDH
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas [2] [3]
- protein localization: data available for COL
- quantitative data / protein copy number per cell: data available for COL
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: SAOUHSC_01618 < SAOUHSC_01619 < xseA < nusB < SAOUHSC_01622 < SAOUHSC_01623 < SAOUHSC_01624predicted SigA promoter [4] : S647 < SAOUHSC_01618 < SAOUHSC_01619 < xseA < nusB < SAOUHSC_01622 < SAOUHSC_01623 < SAOUHSC_01624predicted SigA promoter [4] : S647 < SAOUHSC_01618 < SAOUHSC_01619 < xseA < nusB < SAOUHSC_01622 < SAOUHSC_01623 < SAOUHSC_01624 < S648 < SAOUHSC_01625 < SAOUHSC_01626
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: [4]
Multi-gene expression profiles
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You can add further information about the gene and protein here. [edit]
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
BMC Genomics: 2009, 10;291
[PubMed:19570206] [WorldCat.org] [DOI] (I e) - ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
Proteomics: 2015, 15(21);3648-61
[PubMed:26224020] [WorldCat.org] [DOI] (I p) - ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
Sci Rep: 2017, 7(1);9718
[PubMed:28887440] [WorldCat.org] [DOI] (I e) - ↑ 4.0 4.1 4.2 4.3 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
PLoS Genet: 2016, 12(4);e1005962
[PubMed:27035918] [WorldCat.org] [DOI] (I e)