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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01273
  • pan locus tag?: SAUPAN003607000
  • symbol: mutL
  • pan gene symbol?: mutL
  • synonym:
  • product: DNA mismatch repair protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01273
  • symbol: mutL
  • product: DNA mismatch repair protein
  • replicon: chromosome
  • strand: +
  • coordinates: 1229312..1231321
  • length: 2010
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGGAAAATTAAAGAACTCCAAACCTCATTAGCAAATAAAATCGCAGCAGGTGAAGTA
    GTTGAAAGACCGAGTTCTGTTGTGAAAGAACTGTTGGAAAATGCTATAGATGCAGGCGCT
    ACAGAAATAAGCATTGAAGTAGAGGAATCTGGCGTCCAATCTATTCGCGTAGTCGATAAT
    GGAAGCGGAATTGAAGCGGAAGACTTAGGATTAGTATTTCATAGACATGCGACTAGTAAA
    TTAGATCAAGATGAAGATTTATTTCATATTAGGACATTAGGATTCCGTGGTGAAGCACTA
    GCCAGTATTTCATCAGTTGCTAAAGTAACATTGAAGACTTGCACGGATAATGCTAATGGA
    AATGAAATATATGTAGAAAATGGTGAAATATTAAATCATAAGCCTGCAAAAGCGAAAAAA
    GGAACAGATATACTTGTAGAATCATTATTTTATAATACACCAGCACGTTTAAAATATATT
    AAAAGTTTATACACTGAACTAGGTAAAATAACAGATATTGTCAACAGAATGGCAATGAGC
    CATCCGGACATTCGAATAGCACTCATTTCAGATGGCAAAACAATGTTAAGTACAAATGGT
    TCAGGACGAACTAATGAAGTGATGGCAGAGATTTATGGGATGAAAGTTGCACGAGATTTA
    GTACATATATCTGGAGATACAAGTGATTATCACATTGAAGGTTTTGTTGCAAAGCCTGAA
    CATTCTAGAAGTAATAAGCACTATATTTCTATTTTTATTAATGGACGATACATTAAAAAC
    TTTATGCTAAATAAAGCGATTTTAGAAGGCTATCATACACTCTTAACAATAGGTAGGTTC
    CCGATTTGTTATATTAATATTGAAATGGATCCAATCTTAGTAGACGTAAATGTTCATCCA
    ACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAA
    ATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCA
    AAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAA
    AACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAACAGTAAGCCATTT
    ATGGAAGAAAATCAAAACGATGAGATAGTTATAAAAGAAGATTCATATAATCCATTCGTA
    ACGAAAACATCTGAAAGTTTAATAGCTGATGATGAATCTTCTGGTTATAATAATACACGT
    GAAAAAGATGAAGACTACTTCAAAAAACAACAAGAAATTTTACAAGAAATGGATCAAACA
    TTTGATTCAAATGATGGTACAACTGTGCAAAATTATGAGAATAAAGCGTCTGATGATTAT
    TATGATGTAAACGATATTAAAGGAACAAAAAGTAAAGACCCTAAACGAAGAATTCCATAT
    ATGGAAATTGTTGGACAAGTACATGGAACGTATATTATTGCTCAAAATGAATTTGGCATG
    TACATGATTGACCAGCATGCAGCTCAAGAAAGAATAAAATATGAATATTTTCGAGATAAA
    ATAGGTGAGGTTACCAATGAAGTACAAGATTTATTAATCCCGTTAACATTTCATTTTTCA
    AAAGATGAACAATTAGTCATTGATCAATATAAAAATGAGCTTCAACAAGTAGGTATCATG
    TTAGAACATTTTGGTGGTCATGATTATATTGTAAGTAGCTATCCAGTTTGGTTCCCTAAA
    GATGAAGTAGAAGAAATTATTAAAGATATGATTGAGCTAATTTTGGAAGAGAAAAAAGTA
    GATATCAAAAAATTACGTGAAGATGTAGCAATCATGATGTCATGTAAAAAATCCATTAAA
    GCGAATCATTATTTACAAAAACATGAAATGTCTGATTTAATTGATCAATTAAGAGAAGCG
    GAAGATCCATTTACATGTCCACATGGTCGTCCAATAATCATTAATTTTTCAAAATACGAA
    TTAGAAAAATTATTTAAGCGTGTGATGTAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2010

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01273
  • symbol: MutL
  • description: DNA mismatch repair protein
  • length: 669
  • theoretical pI: 5.05367
  • theoretical MW: 76854.5
  • GRAVY: -0.538715

⊟Function[edit | edit source]

  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA mismatch repair protein MutL (TIGR00585; HMM-score: 338.9)
    and 4 more
    Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 17.5)
    Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 16.1)
    Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 16.1)
    heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 10.8)
  • TheSEED  :
    • DNA mismatch repair protein MutL
    DNA Metabolism DNA repair DNA repair, bacterial MutL-MutS system  DNA mismatch repair protein MutL
  • PFAM:
    no clan defined MutL_C; MutL C terminal dimerisation domain (PF08676; HMM-score: 157.2)
    S5 (CL0329) DNA_mis_repair; DNA mismatch repair protein, C-terminal domain (PF01119; HMM-score: 130.2)
    and 3 more
    His_Kinase_A (CL0025) HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 48.6)
    HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 35.2)
    FixH-like (CL0488) FixH; FixH (PF05751; HMM-score: 11.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005814
    • TAT(Tat/SPI): 0.000706
    • LIPO(Sec/SPII): 0.000471
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEISIEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALASISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYIKSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVARDLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLTIGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKNNLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMEENQNDEIVIKEDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDGTTVQNYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAAQERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGGHDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHYLQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Alexander J O'Neill, Ian Chopra
Insertional inactivation of mutS in Staphylococcus aureus reveals potential for elevated mutation frequencies, although the prevalence of mutators in clinical isolates is low.
J Antimicrob Chemother: 2002, 50(2);161-9
[PubMed:12161395] [WorldCat.org] [DOI] (P p)

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