PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1205 [new locus tag: NWMN_RS06780 ]
• pan locus tag^?: SAUPAN003607000
• symbol: mutL
• pan gene symbol^?: mutL
• synonym:
• product: DNA mismatch repair protein

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1205 [new locus tag: NWMN_RS06780 ]
• symbol: mutL
• product: DNA mismatch repair protein
• replicon: chromosome
• strand: +
• coordinates: 1329268..1331277
• length: 2010
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332184 NCBI
• RefSeq: YP_001332239 NCBI
• BioCyc:
• MicrobesOnline: 3706757 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGGGGAAAATTAAAGAACTCCAAACCTCATTAGCAAATAAAATCGCAGCAGGTGAAGTA
  GTTGAAAGACCGAGTTCTGTTGTGAAAGAACTGTTGGAAAATGCTATAGATGCAGGCGCT
  ACAGAAATAAGCATTGAAGTAGAGGAATCTGGCGTCCAATCTATTCGCGTAGTCGATAAT
  GGAAGCGGAATTGAAGCGGAAGACTTAGGATTAGTATTTCATAGACATGCGACTAGTAAA
  TTAGATCAAGATGAAGATTTATTTCATATTAGGACATTAGGATTCCGTGGTGAAGCACTA
  GCCAGTATTTCATCAGTTGCTAAAGTAACATTGAAGACTTGCACGGATAATGCTAATGGA
  AATGAAATATATGTAGAAAATGGTGAAATATTAAATCATAAGCCTGCAAAAGCGAAAAAA
  GGAACAGATATACTTGTAGAATCATTATTTTATAATACACCAGCACGTTTAAAATATATT
  AAAAGTTTATACACTGAACTAGGTAAAATAACAGATATTGTCAACAGAATGGCAATGAGC
  CATCCGGACATTCGAATAGCACTCATTTCAGATGGCAAAACAATGTTAAGTACAAATGGT
  TCAGGACGAACTAATGAAGTGATGGCAGAGATTTATGGGATGAAAGTTGCACGAGATTTA
  GTACATATATCTGGAGATACAAGTGATTATCACATTGAAGGTTTTGTTGCAAAGCCTGAA
  CATTCTAGAAGTAATAAGCACTATATTTCTATTTTTATTAATGGACGATACATTAAAAAC
  TTTATGCTAAATAAAGCGATTTTAGAAGGCTATCATACACTCTTAACAATAGGTAGGTTC
  CCGATTTGTTATATTAATATTGAAATGGATCCAATCTTAGTAGACGTAAATGTTCATCCA
  ACAAAACTAGAAGTGCGTTTATCAAAAGAAGAGCAACTATACCAATTGATTGTGAGCAAA
  ATACAAGAAGCATTTAAAGACCGTATATTAATTCCTAAAAATAACTTGGATTATGTGCCA
  AAAAAAAATAAAGTATTACATTCATTCGAACAACAAAAAATCGAATTTGAACAAAGACAA
  AACACAGAGAATAACCAAGAGAAGACGTTTTCATCTGAAGAAAGTAACAGTAAGCCATTT
  ATGGAAGAAAATCAAAACGATGAGATAGTTATAAAAGAAGATTCATATAATCCATTCGTA
  ACGAAAACATCTGAAAGTTTAATAGCTGATGATGAATCTTCTGGTTATAATAATACACGT
  GAAAAAGATGAAGACTACTTCAAAAAACAACAAGAAATTTTACAAGAAATGGATCAAACA
  TTTGATTCAAATGATGGTACAACTGTGCAAAATTATGAGAATAAAGCGTCTGATGATTAT
  TATGATGTAAACGATATTAAAGGAACAAAAAGTAAAGACCCTAAACGAAGAATTCCATAT
  ATGGAAATTGTTGGACAAGTACATGGAACGTATATTATTGCTCAAAATGAATTTGGCATG
  TACATGATTGACCAGCATGCAGCTCAAGAAAGAATAAAATATGAATATTTTCGAGATAAA
  ATAGGTGAGGTTACCAATGAAGTACAAGATTTATTAATCCCGTTAACATTTCATTTTTCA
  AAAGATGAACAATTAGTCATTGATCAATATAAAAATGAGCTTCAACAAGTAGGTATCATG
  TTAGAACATTTTGGTGGTCATGATTATATTGTAAGTAGCTATCCAGTTTGGTTCCCTAAA
  GATGAAGTAGAAGAAATTATTAAAGATATGATTGAGCTAATTTTGGAAGAGAAAAAAGTA
  GATATCAAAAAATTACGTGAAGATGTAGCAATCATGATGTCATGTAAAAAATCCATTAAA
  GCGAATCATTATTTACAAAAACATGAAATGTCTGATTTAATTGATCAATTAAGAGAAGCG
  GAAGATCCATTTACATGTCCACATGGTCGTCCAATAATCATTAATTTTTCAAAATACGAA
  TTAGAAAAATTATTTAAGCGTGTGATGTAG
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⊟Protein

⊟General

• locus tag: NWMN_1205 [new locus tag: NWMN_RS06780 ]
• symbol: MutL
• description: DNA mismatch repair protein
• length: 669
• theoretical pI: 5.05367
• theoretical MW: 76854.5
• GRAVY: -0.538715

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair DNA mismatch repair protein MutL (TIGR00585; HMM-score: 338.9)
  and 4 more
  Signal transduction Signal transduction Two-component systems phosphate regulon sensor kinase PhoR (TIGR02966; EC 2.7.3.-; HMM-score: 17.5)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 16.1)
  Signal transduction Signal transduction Two-component systems putative PEP-CTERM system histidine kinase (TIGR02916; EC 2.7.13.3; HMM-score: 16.1)
  heavy metal sensor kinase (TIGR01386; EC 2.7.13.3; HMM-score: 10.8)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined MutL_C; MutL C terminal dimerisation domain (PF08676; HMM-score: 157.2)
  S5 (CL0329) DNA_mis_repair; DNA mismatch repair protein, C-terminal domain (PF01119; HMM-score: 130.2)
  and 3 more
  His_Kinase_A (CL0025) HATPase_c_3; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF13589; HMM-score: 48.6)
  HATPase_c; Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (PF02518; HMM-score: 35.2)
  FixH-like (CL0488) FixH; FixH (PF05751; HMM-score: 11.6)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.005814
  • TAT(Tat/SPI): 0.000706
  • LIPO(Sec/SPII): 0.000471
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221417 NCBI
• RefSeq: YP_001332239 NCBI
• UniProt: A6QGJ5 UniProt

⊟Protein sequence

• MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEISIEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALASISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYIKSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVARDLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLTIGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKNNLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMEENQNDEIVIKEDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDGTTVQNYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAAQERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGGHDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHYLQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: mutS > mutL > glpP

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications