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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_01179
  • pan locus tag?: SAUPAN003494000
  • symbol: SAOUHSC_01179
  • pan gene symbol?: priA
  • synonym:
  • product: primosomal protein N

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_01179
  • symbol: SAOUHSC_01179
  • product: primosomal protein N
  • replicon: chromosome
  • strand: +
  • coordinates: 1129107..1131515
  • length: 2409
  • essential: yes [1] DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGATAGCGAAAGTCATAGTCGATGTCGCGTCGAAGAGCGTTGACTATAAATTTGATTAT
    ATAATTCCCGAACAACTCGAATCTGTCATCCAACCTGGTGTGCGTGTAATTGTACCTTTT
    GGACCAAGAACGATTCAAGGTTATGTAATGGAAGTAACAGCAGAACCTGATGCACAACTT
    GACGTTTCGAAGTTAAAAAAAATCATAGAAGTGAAAGATATACAACCAGAATTAACATCA
    GAATTAATAGCTTTAAGTGAGTGGATGGGTTCAACTCATGTCATTAAACGTATTTCTATG
    CTAGAAGTGATGCTTCCGAGTGCTATTAAAGCGAAGTATAAAAAAGCATTTAAGATGAAA
    GATGACATAGAGCTACCTTCAGCTTTATTACAAAAATTTGATAAGCATGGTTACTATTAT
    TATAAAGATGCGCAAAAAAATAATGATATTCAATTGCTTATGAAGTTGTTAAAAGATGAT
    ATCGTTGAAGAAAAAACGATTCTCACACAAAATATAACTAAAAAAACCAAGCGTGCTGTT
    CGTGTCATTGAAGGGTATCATCCTGATGAAGTATTAGCTAAGTTGGAGAAAGTTATTAAA
    CAATACGATTTGTATGCTTACTTGTCTGAAGAACAACATAAAACAATATTTTTAACTGAT
    ATTGAGGATATGGGCTTTTCAAAATCCAGTTTAGATGGACTTATCAAAAAAGGTTATGTT
    GAAAAATATGACGCGGTTGTTGAAAGAGACCCATTTAAAGATCGTGTTTTCGAACAAGAA
    TCAAAACAGCAATTAACAGAAGACCAATATAAAGCATATGAAGCGATTAAAGCTAAAATT
    GTAAGCCAAGAGCAAGAAACATTTTTACTTCATGGTGTGACGGGATCAGGTAAAACAGAA
    GTATATTTACAAACGATAGAAGATGTTTTAAGCCAAGGAAAACAGGCGATGATGTTAGTT
    CCTGAAATCGCTCTAACACCGCAAATGGTTTTACGCTTCAAACGTCGATTTGGTGATGAC
    GTTGCTGTATTACATTCTGGCTTATCTAATGGGGAACGTTATGATGAATGGCAAAAAATT
    AGGGATGGTCGTGCGAGAGTAAGTGTTGGTGCAAGGTCAAGTGTGTTCGCACCTTTCAAA
    AATTTAGGGTTAATCATCATTGATGAAGAACATGAATCTACATATAAACAAGAAGATTAT
    CCGAGATATCACGCTAGAGAAATTGCCCAATGGCGAAGTGAATATCATCACTGTCCAGTC
    ATTTTAGGAAGTGCAACACCATGTCTTGAAAGTTATGCACGAGCTGAAAAAGGCGTTTAT
    CATTTGCTATCATTACCAAACAGAGTGAACCAACAAGCTTTACCTGAAATTGATATAGTA
    GACATGCGTGAAGAATTGAGTGAAGGTAATCGGTCAATGTTTTCAAAAGATTTACGTGAA
    GCCATACAATTAAGATTAGATCGACAGGAACAAGTTGTTTTATTTTTAAATCGACGTGGT
    TATGCATCGTTTATGTTATGTCGGGATTGTGGATATGTACCGCAATGTCCAAACTGTGAT
    ATTTCATTAACGTATCATAAAACGACAGACTTATTAAAATGTCACTATTGTGGTTACCAA
    GAGACGCCACCGAATCAATGTCCAAATTGTGAGAGTGAACACATTCGACAAGTAGGTACT
    GGTACTCAGAAAGTTGAAGAACTATTGCAACAAGAATTTGAAGATGCGCGCATAATTAGG
    ATGGATGTAGATACAACCTCAAAGAAAGGTGCACATGAAAAGTTATTGACTGAATTCGAA
    AAAGGTAACGGTGACATTTTACTAGGTACTCAGATGATTGCGAAAGGATTAGATTATCCA
    AATATTACTTTAGTTGGTGTGCTGAATGCAGATACAATGTTAAATTTACCTGATTTTCGG
    GCGAGCGAACGTACTTATCAACTATTAACGCAAGTGGCTGGTAGAGCTGGTCGTCATGAA
    AAGGCAGGTCAAGTCATCATTCAAACGTATAATCCAGATCATTATTCAATATTGGATGTT
    CAAAAAAATGATTATTTAACATTTTATCGTCAGGAAATGGAATATCGTAAATTAGGAAAG
    TATCCACCGTATTATTATTTGATTAATTTCACAATCTCACATAAAGAAATGAAGAAGGTT
    ATGGTAGCATCGCAGCATGTTCATAAAATTTTATTACAGCATTTAACAGAAAAAGCGCTT
    GTACTAGGTCCATCTCCGGCAGCACTTGCGAGAATCAACAATGAATTTAGATTCCAAATT
    TTAGTGAAATATAAAAGTGAACCTGGATTATTACAAGCCATTCAGTTTTTAGATGACTAT
    TACCATGAAAAATTTATAAAAGAAAAATTAGCATTGAAGATTGATATTGATCCACAGATG
    ATGATGTAA
    60
    120
    180
    240
    300
    360
    420
    480
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    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1560
    1620
    1680
    1740
    1800
    1860
    1920
    1980
    2040
    2100
    2160
    2220
    2280
    2340
    2400
    2409

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_01179
  • symbol: SAOUHSC_01179
  • description: primosomal protein N
  • length: 802
  • theoretical pI: 6.74374
  • theoretical MW: 92521.8
  • GRAVY: -0.421072

⊟Function[edit | edit source]

  • reaction:
    EC 3.6.4.-?  ExPASy
  • TIGRFAM:
    Genetic information processing DNA metabolism DNA replication, recombination, and repair primosomal protein N' (TIGR00595; EC 3.6.1.-; HMM-score: 712.9)
    and 8 more
    Genetic information processing DNA metabolism DNA replication, recombination, and repair ATP-dependent DNA helicase RecG (TIGR00643; EC 3.6.4.12; HMM-score: 79.2)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair transcription-repair coupling factor (TIGR00580; EC 3.6.1.-; HMM-score: 70.8)
    Genetic information processing DNA metabolism DNA replication, recombination, and repair excinuclease ABC subunit B (TIGR00631; EC 3.1.25.-; HMM-score: 49.8)
    type IV conjugative transfer system coupling protein TraD (TIGR02759; HMM-score: 14.4)
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA 2-selenouridine synthase (TIGR03167; EC 2.9.1.-; HMM-score: 13.9)
    KaiC domain protein, AF_0351 family (TIGR03880; HMM-score: 12.4)
    Unknown function General putative alkylphosphonate utilization operon protein PhnA (TIGR00686; HMM-score: 12.1)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides 2'-Deoxyribonucleotide metabolism anaerobic ribonucleoside-triphosphate reductase (TIGR02487; EC 1.17.4.2; HMM-score: 11.6)
  • TheSEED  :
    • Helicase PriA essential for oriC/DnaA-independent DNA replication
    DNA Metabolism DNA replication DNA-replication  Helicase PriA essential for oriC/DnaA-independent DNA replication
  • PFAM:
    P-loop_NTPase (CL0023) ResIII; Type III restriction enzyme, res subunit (PF04851; HMM-score: 64.5)
    DEAD; DEAD/DEAH box helicase (PF00270; HMM-score: 54.6)
    and 15 more
    Helicase_C; Helicase conserved C-terminal domain (PF00271; HMM-score: 45)
    PIF1; PIF1-like helicase (PF05970; HMM-score: 19.5)
    Zn_Beta_Ribbon (CL0167) Zn_Tnp_IS1595; Transposase zinc-ribbon domain (PF12760; HMM-score: 15.1)
    P-loop_NTPase (CL0023) TrwB_AAD_bind; Type IV secretion-system coupling protein DNA-binding domain (PF10412; HMM-score: 14.4)
    T2SSE; Type II/IV secretion system protein (PF00437; HMM-score: 13.3)
    Microtub_bd; Microtubule binding (PF16796; HMM-score: 12.9)
    AAA_30; AAA domain (PF13604; HMM-score: 12.3)
    Zn_Beta_Ribbon (CL0167) zinc-ribbons_6; zinc-ribbons (PF07191; HMM-score: 11.5)
    DNA_RNApol_7kD; DNA directed RNA polymerase, 7 kDa subunit (PF03604; HMM-score: 10.4)
    DUF1451; Zinc-ribbon containing domain (PF07295; HMM-score: 9)
    zf-TFIIB; Transcription factor zinc-finger (PF13453; HMM-score: 8)
    no clan defined Cas_Csa5; CRISPR-associated protein (Cas_Csa5) (PF09702; HMM-score: 7.5)
    Zn_Beta_Ribbon (CL0167) TF_Zn_Ribbon; TFIIB zinc-binding (PF08271; HMM-score: 7.3)
    PhnA_Zn_Ribbon; PhnA Zinc-Ribbon (PF08274; HMM-score: 7.3)
    no clan defined HypA; Hydrogenase/urease nickel incorporation, metallochaperone, hypA (PF01155; HMM-score: 6.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.005634
    • TAT(Tat/SPI): 0.00011
    • LIPO(Sec/SPII): 0.000239
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MIAKVIVDVASKSVDYKFDYIIPEQLESVIQPGVRVIVPFGPRTIQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLEVMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDDIVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFLTDIEDMGFSKSSLDGLIKKGYVEKYDAVVERDPFKDRVFEQESKQQLTEDQYKAYEAIKAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFKRRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHESTYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVNQQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCRDCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVEELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITLVGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQKNDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMVASQHVHKILLQHLTEKALVLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDPQMMM

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [2] [3]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Roy R Chaudhuri, Andrew G Allen, Paul J Owen, Gil Shalom, Karl Stone, Marcus Harrison, Timothy A Burgis, Michael Lockyer, Jorge Garcia-Lara, Simon J Foster, Stephen J Pleasance, Sarah E Peters, Duncan J Maskell, Ian G Charles
    Comprehensive identification of essential Staphylococcus aureus genes using Transposon-Mediated Differential Hybridisation (TMDH).
    BMC Genomics: 2009, 10;291
    [PubMed:19570206] [WorldCat.org] [DOI] (I e)
  2. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  3. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  4. ↑ 4.0 4.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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