PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS12215 [old locus tag: SACOL2323 ]
• pan locus tag^?: SAUPAN005817000
• symbol: SACOL_RS12215
• pan gene symbol^?: hutI
• synonym:
• product: imidazolonepropionase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS12215 [old locus tag: SACOL2323 ]
• symbol: SACOL_RS12215
• product: imidazolonepropionase
• replicon: chromosome
• strand: -
• coordinates: 2384501..2385739
• length: 1239
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2384501..2385739) NCBI
• BioCyc: SACOL_RS12215 BioCyc
• MicrobesOnline: see SACOL2323

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAATGATTTAATAATTAATCATATAGCAGAATTAATTTTACCGAGATCAACAGATAAA
  CCTTTGAAGGGTAAGGAATTAGATGAATTAAATGTTGTGAAAAATGGTACAGTTGTCATT
  AAAGATGGCAAAATTGTATATGCTGGAACACATACAGATGACTACGATGCGACTGAGACG
  ATTGATGCTAGTGGGAAGGTAGTGTCCCCAGCATTAGTAGATGCACATACCCATTTAACA
  TTTGGTGGATCTCGAGAACATGAGATGTCATTAAAACGTCAAGGTAAGTCTTACCTTGAA
  ATATTAGAAATGGGTGGTGGCATATTATCTACCGTTAATGCTACTAGAGAAACATCCGAA
  GATGACTTGTTTAAAAAAGCAGAGCATGATTTGCTTACTATGATTAAACACGGCGTGCTT
  GCAGTTGAAAGTAAGAGTGGTTATGGATTAGATAGAGAAAATGAACTGAAGCAATTGAAA
  GTGTCTAATCGTTTAGCTGAGAAATATGATTTAGACATGAAACATACTTTCCTAGGGCCT
  CATGCTGTACCTAAAGAGGCAAGTTCAAATGAGGCATTTTTAGAAGAAATGATTGCGTTA
  CTTCCGGAAGTAAAACAATATGCAGACTTTGCGGATATTTTCTGTGAAACAGGTGTATTT
  ACAATAGAACAATCGCAACATTATATGCAAAAAGCCAAAGAAGCAGGTTTTAAAGTGAAA
  ATACATGCGGATGAAATTGATCCGTTAGGCGGACTGGAATTAGCAATTGATGAACAAGCA
  ATATCAGCAGACCACTTAGTAGCTTCTAGTGATAAAGGAAAAGAAAAGTTGCGAAATAGT
  GATACTGTAGCTGTCCTCTTACCTGCAACGACGTTCTATTTAGGTAAAGAAGATTATGCA
  GATGCAAGAGGCATGCTTGACAATAACGGTGCGATTGCATTAGCAACTGATTATAACCCT
  GGTAGTAGTGTCACAAACAACTTACAACTTGTTATGGCGATTGCAGCCTTGAAATTAAAG
  CTATCACCTAATGAAGTTTGGAATGCTGTTACGGTCAATGCTGCTAAAGCAATAGACATT
  AACGCGGGTACAATTAACACAGGTGATAAGGCTAATTTAGTAATTTGGGATGCACCAAAT
  CATGAATATATTCCATATCATTTTGGTATCAATCACGCAGAAAAAGTTATTAAAGACGGT
  AAGGTCATCGTAGATAACACAGTATCTTTTAAAGCATAA
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⊟Protein

⊟General

• locus tag: SACOL_RS12215 [old locus tag: SACOL2323 ]
• symbol: SACOL_RS12215
• description: imidazolonepropionase
• length: 412
• theoretical pI: 5.03086
• theoretical MW: 45038.7
• GRAVY: -0.258738

⊟Function

• reaction:
  EC 3.5.2.7?  ExPASy
  Imidazolonepropionase (S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H₂O = N-formimidoyl-L-glutamate + H⁺
• TIGRFAM:
  Metabolism Energy metabolism Amino acids and amines imidazolonepropionase (TIGR01224; EC 3.5.2.7; HMM-score: 434.1)
  and 11 more
  Metabolism Energy metabolism Other phosphonate metabolism protein PhnM (TIGR02318; HMM-score: 45.5)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine deaminase (TIGR01178; EC 3.5.4.2; HMM-score: 38.6)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other guanine deaminase (TIGR02967; EC 3.5.4.3; HMM-score: 37.3)
  allantoinase (TIGR03178; EC 3.5.2.5; HMM-score: 36.4)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis dihydroorotase, multifunctional complex type (TIGR00857; EC 3.5.2.3; HMM-score: 35.9)
  Metabolism Central intermediary metabolism Amino sugars N-acetylglucosamine-6-phosphate deacetylase (TIGR00221; EC 3.5.1.25; HMM-score: 35.7)
  formylmethanofuran dehydrogenase subunit A (TIGR03121; EC 1.2.99.5; HMM-score: 33.1)
  dihydropyrimidinase (TIGR02033; EC 3.5.2.2; HMM-score: 33)
  Hypothetical proteins Conserved putative amidohydrolase, EF_0837/AHA_3915 family (TIGR03583; HMM-score: 32.3)
  putative selenium metabolism protein SsnA (TIGR03314; HMM-score: 26.3)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides beta-aspartyl peptidase (TIGR01975; EC 3.4.19.5; HMM-score: 18.8)
• TheSEED: see SACOL2323
• PFAM:
  Amidohydrolase (CL0034) Amidohydro_1; Amidohydrolase family (PF01979; HMM-score: 72.2)
  Amidohydro_3; Amidohydrolase family (PF07969; HMM-score: 60.4)
  and 1 more
  Phage_TACs (CL0567) Phage_TAC_6; Phage tail assembly chaperone protein, TAC (PF09550; HMM-score: 14.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Fe²⁺, Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.006786
  • TAT(Tat/SPI): 0.00028
  • LIPO(Sec/SPII): 0.00073
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446921511 NCBI
• RefSeq: WP_000998767 NCBI
• UniProt: see SACOL2323

⊟Protein sequence

• MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGKIVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEILEMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLKVSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETGVFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEKLRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAIAALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINHAEKVIKDGKVIVDNTVSFKA

⊟Experimental data

• experimentally validated: see SACOL2323
• protein localization: see SACOL2323
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA, HutR* see SACOL2323

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications