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NCBI: 03-AUG-2016

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus NCTC8325
  • locus tag: SAOUHSC_02606
  • pan locus tag?: SAUPAN005817000
  • symbol: SAOUHSC_02606
  • pan gene symbol?: hutI
  • synonym:
  • product: imidazolonepropionase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SAOUHSC_02606
  • symbol: SAOUHSC_02606
  • product: imidazolonepropionase
  • replicon: chromosome
  • strand: -
  • coordinates: 2395166..2396404
  • length: 1239
  • essential: no DEG other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATGATTTAATAATTAATCATATAGCAGAATTAATTTTACCGAGATCAACAGATAAA
    CCTTTGAAGGGTAAGGAATTAGATGAATTAAATGTTGTGAAAAATGGTACAGTTGTCATT
    AAAGATGGCAAAATTGTATATGCTGGAACACATACAGATGACTACGATGCGACTGAGACG
    ATTGATGCTAGTGGGAAGGTAGTGTCCCCAGCATTAGTAGATGCACATACCCATTTAACA
    TTTGGTGGATCTCGAGAACATGAGATGTCATTAAAACGTCAAGGTAAGTCTTACCTTGAA
    ATATTAGAAATGGGTGGTGGCATATTATCTACCGTTAATGCTACTAGAGAAACATCCGAA
    GATGACTTGTTTAAAAAAGCAGAGCATGATTTGCTTACTATGATTAAACACGGCGTGCTT
    GCAGTTGAAAGTAAGAGTGGTTATGGATTAGATAGAGAAAATGAACTGAAGCAATTGAAA
    GTGTCTAATCGTTTAGCTGAGAAATATGATTTAGACATGAAACATACTTTCCTAGGGCCT
    CATGCTGTACCTAAAGAGGCAAGTTCAAATGAGGCATTTTTAGAAGAAATGATTGCGTTA
    CTTCCGGAAGTAAAACAATATGCAGACTTTGCGGATATTTTCTGTGAAACAGGTGTATTT
    ACAATAGAACAATCGCAACATTATATGCAAAAAGCCAAAGAAGCAGGTTTTAAAGTGAAA
    ATACATGCGGATGAAATTGATCCGTTAGGCGGACTGGAATTAGCAATTGATGAACAAGCA
    ATATCAGCAGACCACTTAGTAGCTTCTAGTGATAAAGGAAAAGAAAAGTTGCGAAATAGT
    GATACTGTAGCTGTCCTCTTACCTGCAACGACGTTCTATTTAGGTAAAGAAGATTATGCA
    GATGCAAGAGGCATGCTTGACAATAACGGTGCGATTGCATTAGCAACTGATTATAACCCT
    GGTAGTAGTGTCACAAACAACTTACAACTTGTTATGGCGATTGCAGCCTTGAAATTAAAG
    CTATCACCTAATGAAGTTTGGAATGCTGTTACGGTCAATGCTGCTAAAGCAATAGACATT
    AACGCGGGTACAATTAACACAGGTGATAAGGCTAATTTAGTAATTTGGGATGCACCAAAT
    CATGAATATATTCCATATCATTTTGGTATCAATCACGCAGAAAAAGTTATTAAAGACGGT
    AAGGTCATCGTAGATAACACAGTATCTTTTAAAGCATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1239

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SAOUHSC_02606
  • symbol: SAOUHSC_02606
  • description: imidazolonepropionase
  • length: 412
  • theoretical pI: 5.03086
  • theoretical MW: 45038.7
  • GRAVY: -0.258738

⊟Function[edit | edit source]

  • reaction:
    EC 3.5.2.7?  ExPASy
    Imidazolonepropionase (S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+
  • TIGRFAM:
    Metabolism Energy metabolism Amino acids and amines imidazolonepropionase (TIGR01224; EC 3.5.2.7; HMM-score: 434.1)
    and 11 more
    Metabolism Energy metabolism Other phosphonate metabolism protein PhnM (TIGR02318; HMM-score: 45.5)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine deaminase (TIGR01178; EC 3.5.4.2; HMM-score: 38.6)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Other guanine deaminase (TIGR02967; EC 3.5.4.3; HMM-score: 37.3)
    allantoinase (TIGR03178; EC 3.5.2.5; HMM-score: 36.4)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis dihydroorotase, multifunctional complex type (TIGR00857; EC 3.5.2.3; HMM-score: 35.9)
    Metabolism Central intermediary metabolism Amino sugars N-acetylglucosamine-6-phosphate deacetylase (TIGR00221; EC 3.5.1.25; HMM-score: 35.7)
    formylmethanofuran dehydrogenase subunit A (TIGR03121; EC 1.2.99.5; HMM-score: 33.1)
    dihydropyrimidinase (TIGR02033; EC 3.5.2.2; HMM-score: 33)
    Hypothetical proteins Conserved putative amidohydrolase, EF_0837/AHA_3915 family (TIGR03583; HMM-score: 32.3)
    putative selenium metabolism protein SsnA (TIGR03314; HMM-score: 26.3)
    Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides beta-aspartyl peptidase (TIGR01975; EC 3.4.19.5; HMM-score: 18.8)
  • TheSEED  :
    • Imidazolonepropionase (EC 3.5.2.7)
    Amino Acids and Derivatives Histidine Metabolism Histidine Degradation  Imidazolonepropionase (EC 3.5.2.7)
  • PFAM:
    Amidohydrolase (CL0034) Amidohydro_1; Amidohydrolase family (PF01979; HMM-score: 72.2)
    Amidohydro_3; Amidohydrolase family (PF07969; HMM-score: 60.4)
    and 1 more
    Phage_TACs (CL0567) Phage_TAC_6; Phage tail assembly chaperone protein, TAC (PF09550; HMM-score: 14.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Fe2+, Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.006786
    • TAT(Tat/SPI): 0.00028
    • LIPO(Sec/SPII): 0.00073
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNDLIINHIAELILPRSTDKPLKGKELDELNVVKNGTVVIKDGKIVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEILEMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLKVSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETGVFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEKLRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAIAALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINHAEKVIKDGKVIVDNTVSFKA

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas [1] [2]
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: CcpA* regulon, HutR* (activation) regulon
    CcpA*(TF)important in Carbon catabolism; RegPrecise    transcription unit transferred from N315 data RegPrecise 
    HutR*(TF)important in Histidine utilization; RegPrecise    transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Maren Depke, Stephan Michalik, Alexander Rabe, Kristin Surmann, Lars Brinkmann, Nico Jehmlich, Jörg Bernhardt, Michael Hecker, Bernd Wollscheid, Zhi Sun, Robert L Moritz, Uwe Völker, Frank Schmidt
    A peptide resource for the analysis of Staphylococcus aureus in host-pathogen interaction studies.
    Proteomics: 2015, 15(21);3648-61
    [PubMed:26224020] [WorldCat.org] [DOI] (I p)
  2. ↑ Stephan Michalik, Maren Depke, Annette Murr, Manuela Gesell Salazar, Ulrike Kusebauch, Zhi Sun, Tanja C Meyer, Kristin Surmann, Henrike Pförtner, Petra Hildebrandt, Stefan Weiss, Laura Marcela Palma Medina, Melanie Gutjahr, Elke Hammer, Dörte Becher, Thomas Pribyl, Sven Hammerschmidt, Eric W Deutsch, Samuel L Bader, Michael Hecker, Robert L Moritz, Ulrike Mäder, Uwe Völker, Frank Schmidt
    A global Staphylococcus aureus proteome resource applied to the in vivo characterization of host-pathogen interactions.
    Sci Rep: 2017, 7(1);9718
    [PubMed:28887440] [WorldCat.org] [DOI] (I e)
  3. ↑ 3.0 3.1 Ulrike Mäder, Pierre Nicolas, Maren Depke, Jan Pané-Farré, Michel Debarbouille, Magdalena M van der Kooi-Pol, Cyprien Guérin, Sandra Dérozier, Aurelia Hiron, Hanne Jarmer, Aurélie Leduc, Stephan Michalik, Ewoud Reilman, Marc Schaffer, Frank Schmidt, Philippe Bessières, Philippe Noirot, Michael Hecker, Tarek Msadek, Uwe Völker, Jan Maarten van Dijl
    Staphylococcus aureus Transcriptome Architecture: From Laboratory to Infection-Mimicking Conditions.
    PLoS Genet: 2016, 12(4);e1005962
    [PubMed:27035918] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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