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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL_RS10580 [old locus tag: SACOL2026 ]
- pan locus tag?: SAUPAN005281000
- symbol: SACOL_RS10580
- pan gene symbol?: agrA
- synonym:
- product: DNA-binding response regulator
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL_RS10580 [old locus tag: SACOL2026 ]
- symbol: SACOL_RS10580
- product: DNA-binding response regulator
- replicon: chromosome
- strand: +
- coordinates: 2085180..2085896
- length: 717
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
- 1
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661ATGAAAATTTTCATTTGCGAAGACGATCCAAAACAAAGAGAAAACATGGTTACCATTATT
AAAAATTATATAATGATAGAAGAAAAGCCTATGGAAATTGCCCTCGCAACTGATAATCCT
TATGAGGTGCTTGAGCAAGCTAAAAATATGAATGACATAGGCTGTTACTTTTTAGATATT
CAACTTTCAACTGATATTAATGGTATCAAATTAGGCAGTGAAATTCGTAAGCATGACCCA
GTTGGTAACATTATTTTCGTTACGAGTCACAGTGAACTTACCTATTTAACATTTGTCTAC
AAAGTTGCAGCGATGGATTTTATTTTTAAAGATGATCCAGCTGAATTAAGAACTCGAATT
ATAGACTGTTTAGAAACTGCACATACACGCTTACAATTGTTGTCTAAAGATAATAGCGTT
GAAACGATTGAATTAAAACGTGGCAGTAATTCAGTGTATGTTCAATATGATGATATTATG
TTTTTTGAATCATCAACAAAATCTCACAGACTCATTGCCCATTTAGATAACCGTCAAATT
GAATTTTATGGTAATTTAAAAGAACTGAGTCAATTAGATGATCGTTTCTTTAGATGTCAT
AATAGCTTTGTCGTCAATCGCCATAATATTGAATCTATAGATTCGAAAGAGCGAATTGTC
TATTTTAAAAATAAAGAACACTGCTATGCATCGGTGAGAAACGTTAAAAAAATATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL_RS10580 [old locus tag: SACOL2026 ]
- symbol: SACOL_RS10580
- description: DNA-binding response regulator
- length: 238
- theoretical pI: 6.10039
- theoretical MW: 27905.7
- GRAVY: -0.378571
⊟Function[edit | edit source]
- TIGRFAM: Central intermediary metabolism Nitrogen metabolism nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 11.4)Regulatory functions DNA interactions nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 11.4)Signal transduction Two-component systems nitrogen regulation protein NR(I) (TIGR01818; HMM-score: 11.4)
- TheSEED: see SACOL2026
- PFAM: CheY (CL0304) Response_reg; Response regulator receiver domain (PF00072; HMM-score: 86.9)Tudor (CL0049) LytTR; LytTr DNA-binding domain (PF04397; HMM-score: 72.5)and 2 moreno clan defined MinE; Septum formation topological specificity factor MinE (PF03776; HMM-score: 14.4)TPR (CL0020) Suf; Suppressor of forked protein (Suf) (PF05843; HMM-score: 12.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
- genes regulated by AgrA, TF important in Virulence, Quorum sensing: see SACOL2026
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 10
- Cytoplasmic Membrane Score: 0
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.00521
- TAT(Tat/SPI): 0.000153
- LIPO(Sec/SPII): 0.000698
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVLEQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKVAAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIMFFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKERIVYFKNKEHCYASVRNVKKI
⊟Experimental data[edit | edit source]
- experimentally validated: see SACOL2026
- protein localization: see SACOL2026
- quantitative data / protein copy number per cell:
- interaction partners:
SACOL_RS10255 (gatA) glutamyl-tRNA(Gln) amidotransferase subunit A [1] (data from MRSA252) SACOL_RS00185 PBP2a family beta-lactam-resistant peptidoglycan transpeptidase MecA [1] (data from MRSA252) SACOL_RS02410 type I restriction-modification system subunit M [1] (data from MRSA252) SACOL_RS03030 50S ribosomal protein L1 [1] (data from MRSA252) SACOL_RS03755 LysR family transcriptional regulator [1] (data from MRSA252) SACOL_RS06505 30S ribosomal protein S2 [1] (data from MRSA252) SACOL_RS07705 DNA-binding protein HU [1] (data from MRSA252) SACOL_RS08340 molecular chaperone DnaJ [1] (data from MRSA252) SACOL_RS08865 aldehyde dehydrogenase [1] (data from MRSA252) SACOL_RS08995 universal stress protein UspA [1] (data from MRSA252) SACOL_RS11695 30S ribosomal protein S5 [1] (data from MRSA252) SACOL_RS11750 30S ribosomal protein S3 [1] (data from MRSA252) SACOL_RS11785 30S ribosomal protein S10 [1] (data from MRSA252) SACOL_RS13365 pyruvate oxidase [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator: AgrA, CodY see SACOL2026
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)