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NCBI: 02-MAR-2017
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL_RS10325 [old locus tag: SACOL1975 ]
- pan locus tag?: SAUPAN004946000
- symbol: SACOL_RS10325
- pan gene symbol?: pncB
- synonym:
- product: nicotinate phosphoribosyltransferase
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL_RS10325 [old locus tag: SACOL1975 ]
- symbol: SACOL_RS10325
- product: nicotinate phosphoribosyltransferase
- replicon: chromosome
- strand: -
- coordinates: 2037975..2039444
- length: 1470
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1441GTGTACCAATTAGAAGACGACAGTTTAATGTTACATAATGACTTATATCAAATAAATATG
GCTGAAAGTTATTGGAATGATAATATTCATGAAAAAATGGCTGTATTTGATTTGTATTTT
AGAAAAATGCCATTTAATAGTGGCTATGCTGTTTTTAATGGTTTAAAACGCGTCATTGAT
TTTATAGAACATTTTGGATTTTCAGAATCTGATTTGGAATATTTAAAGTCTATTGGCTAC
AAGGATGATTTCTTATCATATTTAAAAGATTTAAAATTCACAGGCAGCATCCGTTCGATG
CAAGAAGGCGAATTATGCTTTGGTAACGAACCATTGTTACGCGTAGAAGCACCATTGATT
CAAGCGCAATTAATAGAAACAATTTTATTAAACATTGTAAATTTCCATACATTAATTACA
ACAAAGGCTAGCAGAATTCGTCAAATTGCATCAAATGATAAATTAATGGAGTTTGGTACA
CGTCGTGCGCAAGAAATTGATGCAGCATTGTGGGGCGCTAGAGCTGCTTACATCGGGGGC
TTTGATTCTACAAGTAATGTTAGGGCGGGGAAATTATTTGGTATACCTGTGTCTGGTACA
CATGCACATGCATTTGTCCAAACTTATGGAGACGAATATGTTGCCTTCAAAAAATATGCT
GAAAGACATAAAAATTGTGTGTTCCTAGTAGATACATTCCATACTTTAAAATCTGGCGTG
CCAAATGCAATAAAAGTTGCAAAAGAATTAGGTGACAAAATTAACTTTGTAGGTATTCGA
TTAGATTCTGGAGATATCGCTTATTTATCTAAAGAGGCAAGACGTATGCTTGATGAAGCA
GGATTTACTGAAACTAAAATTATCGCGTCTAATGATTTGGATGAAGAAACGATTACGAGT
TTGAAAGCACAAGGTGCAAAAGTAGATTCTTGGGGCGTTGGTACAAAGCTGATTACAGGA
TACGATCAACCAGCATTAGGTGCAGTATATAAACTTGTAGCTATTGAAAATGAAGATGGT
TCATATAGTGATCGTATTAAATTATCAAATAACGCTGAAAAGGTTACGACGCCAGGTAAG
AAAAATGTATATCGCATTATAAACAAGAAAACAGGTAAGGCAGAAGGCGATTATATTACT
TTGGAAAATGAAAATCCATACGATGAACAACCTTTAAAATTATTCCATCCAGTGCATACT
TATAAAATGAAATTTATAAAATCTTTCGAAGCCATTGATTTGCATCATAATATTTATGAA
AATGGTAAATTAGTATATCAAATGCCAACAGAAGATGAATCACGTGAATATTTAGCACTA
GGATTACAATCTATTTGGGATGAAAATAAGCGTTTCCTGAATCCACAAGAATATCCAGTC
GATTTAAGCAAGGCATGTTGGGATAATAAACATAAACGTATTTTTGAAGTTGCGGAACAC
GTTAAGGAGATGGAAGAAGATAATGAGTAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL_RS10325 [old locus tag: SACOL1975 ]
- symbol: SACOL_RS10325
- description: nicotinate phosphoribosyltransferase
- length: 489
- theoretical pI: 5.51423
- theoretical MW: 55891.9
- GRAVY: -0.434151
⊟Function[edit | edit source]
- reaction: EC 2.4.2.11? ExPASyTransferred entry: 6.3.4.21EC 6.3.4.21? ExPASyNicotinate phosphoribosyltransferase Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides nicotinate phosphoribosyltransferase (TIGR01513; EC 6.3.4.21; HMM-score: 596.1)and 1 moreBiosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides nicotinate phosphoribosyltransferase (TIGR01514; EC 6.3.4.21; HMM-score: 63.6)
- TheSEED: see SACOL1975
- PFAM: TIM_barrel (CL0036) NAPRTase; Nicotinate phosphoribosyltransferase (NAPRTase) family (PF04095; HMM-score: 41.6)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP:
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.001819
- TAT(Tat/SPI): 0.000127
- LIPO(Sec/SPII): 0.000268
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMPFNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQEGELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGTRRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKKYAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRMLDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVAIENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPLKLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLALGLQSIWDENKRFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE
⊟Experimental data[edit | edit source]
- experimentally validated: see SACOL1975
- protein localization: see SACOL1975
- quantitative data / protein copy number per cell: see SACOL1975
- interaction partners:
SACOL_RS03030 50S ribosomal protein L1 [1] (data from MRSA252) SACOL_RS06420 50S ribosomal protein L19 [1] (data from MRSA252) SACOL_RS07705 DNA-binding protein HU [1] (data from MRSA252) SACOL_RS08680 50S ribosomal protein L21 [1] (data from MRSA252) SACOL_RS09045 30S ribosomal protein S4 [1] (data from MRSA252) SACOL_RS11655 30S ribosomal protein S11 [1] (data from MRSA252) SACOL_RS11685 50S ribosomal protein L15 [1] (data from MRSA252) SACOL_RS11705 50S ribosomal protein L6 [1] (data from MRSA252) SACOL_RS11720 50S ribosomal protein L5 [1] (data from MRSA252) SACOL_RS11735 30S ribosomal protein S17 [1] (data from MRSA252) SACOL_RS11745 50S ribosomal protein L16 [1] (data from MRSA252) SACOL_RS11755 50S ribosomal protein L22 [1] (data from MRSA252) SACOL_RS11765 50S ribosomal protein L2 [1] (data from MRSA252) SACOL_RS11780 50S ribosomal protein L3 [1] (data from MRSA252)
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
J Proteome Res: 2011, 10(3);1139-50
[PubMed:21166474] [WorldCat.org] [DOI] (I p)