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NCBI: 06-JUL-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus Newman
  • locus tag: NWMN_1851 [new locus tag: NWMN_RS10635 ]
  • pan locus tag?: SAUPAN004946000
  • symbol: nadC
  • pan gene symbol?: pncB
  • synonym:
  • product: nicotinate phosphoribosyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: NWMN_1851 [new locus tag: NWMN_RS10635 ]
  • symbol: nadC
  • product: nicotinate phosphoribosyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 2062299..2063768
  • length: 1470
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    GTGTACCAATTAGAAGACGACAGTTTAATGTTACATAATGACTTATATCAAATAAATATG
    GCTGAAAGTTATTGGAATGATAATATTCATGAAAAAATGGCTGTATTTGATTTGTATTTT
    AGAAAAATGCCATTTAATAGTGGCTATGCTGTTTTTAATGGTTTAAAACGCGTCATTGAT
    TTTATAGAACATTTTGGATTTTCAGAATCTGATTTGGAATATTTAAAGTCTATTGGCTAC
    AAGGATGATTTCTTATCATATTTAAAAGATTTAAAATTCACAGGCAGCATCCGTTCGATG
    CAAGAAGGCGAATTATGCTTTGGTAACGAACCATTGTTACGCGTAGAAGCACCATTGATT
    CAAGCGCAATTAATAGAAACAATTTTATTAAACATTGTAAATTTCCATACATTAATTACA
    ACAAAGGCTAGCAGAATTCGTCAAATTGCATCAAATGATAAATTAATGGAGTTTGGTACA
    CGTCGTGCGCAAGAAATTGATGCAGCATTGTGGGGCGCTAGAGCTGCTTACATCGGGGGC
    TTTGATTCTACAAGTAATGTTAGGGCGGGGAAATTATTTGGTATACCTGTGTCTGGTACA
    CATGCACATGCATTTGTCCAAACTTATGGAGACGAATATGTTGCCTTCAAAAAATATGCT
    GAAAGACATAAAAATTGTGTGTTCCTAGTAGATACATTCCATACTTTAAAATCTGGCGTG
    CCAAATGCAATAAAAGTTGCAAAAGAATTAGGTGACAAAATTAACTTTGTAGGTATTCGA
    TTAGATTCTGGAGATATCGCTTATTTATCTAAAGAGGCAAGACGTATGCTTGATGAAGCA
    GGATTTACTGAAACTAAAATTATCGCGTCTAATGATTTGGATGAAGAAACGATTACGAGT
    TTGAAAGCACAAGGTGCAAAAGTAGATTCTTGGGGCGTTGGTACAAAGCTGATTACAGGA
    TACGATCAACCAGCATTAGGTGCAGTATATAAACTTGTAGCTATTGAAAATGAAGATGGT
    TCATATAGTGATCGTATTAAATTATCAAATAACGCTGAAAAGGTTACGACGCCAGGTAAG
    AAAAATGTATATCGCATTATAAACAAGAAAACAGGTAAGGCAGAAGGCGATTATATTACT
    TTGGAAAATGAAAATCCATACGATGAACAACCTTTAAAATTATTCCATCCAGTGCATACT
    TATAAAATGAAATTTATAAAATCTTTCGAAGCCATTGATTTGCATCATAATATTTATGAA
    AATGGTAAATTAGTATATCAAATGCCAACAGAAGATGAATCACGTGAATATTTAGCACTA
    GGATTACAATCTATTTGGGATGAAAATAAGCGTTTCCTGAATCCACAAGAATATCCAGTC
    GATTTAAGCAAGGCATGTTGGGATAATAAACATAAACGTATTTTTGAAGTTGCGGAACAC
    GTTAAGGAGATGGAAGAAGATAATGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1470

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: NWMN_1851 [new locus tag: NWMN_RS10635 ]
  • symbol: NadC
  • description: nicotinate phosphoribosyltransferase
  • length: 489
  • theoretical pI: 5.51423
  • theoretical MW: 55891.9
  • GRAVY: -0.434151

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.2.11?  ExPASy
    Transferred entry: 6.3.4.21
    EC 6.3.4.21?  ExPASy
    Nicotinate phosphoribosyltransferase Nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides nicotinate phosphoribosyltransferase (TIGR01513; EC 6.3.4.21; HMM-score: 596.1)
    and 1 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridine nucleotides nicotinate phosphoribosyltransferase (TIGR01514; EC 6.3.4.21; HMM-score: 63.6)
  • TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
  • PFAM:
    TIM_barrel (CL0036) NAPRTase; Nicotinate phosphoribosyltransferase (NAPRTase) family (PF04095; HMM-score: 41.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001819
    • TAT(Tat/SPI): 0.000127
    • LIPO(Sec/SPII): 0.000268
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMPFNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQEGELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGTRRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKKYAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRMLDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVAIENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPLKLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLALGLQSIWDENKRFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell: data available for COL
  • interaction partners:
    NWMN_0500(rplA)50S ribosomal protein L1  [1] (data from MRSA252)
    NWMN_2149(rplB)50S ribosomal protein L2  [1] (data from MRSA252)
    NWMN_2152(rplC)50S ribosomal protein L3  [1] (data from MRSA252)
    NWMN_2140(rplE)50S ribosomal protein L5  [1] (data from MRSA252)
    NWMN_2137(rplF)50S ribosomal protein L6  [1] (data from MRSA252)
    NWMN_2133(rplO)50S ribosomal protein L15  [1] (data from MRSA252)
    NWMN_2145(rplP)50S ribosomal protein L16  [1] (data from MRSA252)
    NWMN_1151(rplS)50S ribosomal protein L19  [1] (data from MRSA252)
    NWMN_1549(rplU)50S ribosomal protein L21  [1] (data from MRSA252)
    NWMN_2147(rplV)50S ribosomal protein L22  [1] (data from MRSA252)
    NWMN_1613(rpsD)30S ribosomal protein S4  [1] (data from MRSA252)
    NWMN_2127(rpsK)30S ribosomal protein S11  [1] (data from MRSA252)
    NWMN_2143(rpsQ)30S ribosomal protein S17  [1] (data from MRSA252)
    NWMN_1382DNA-binding protein HU  [1] (data from MRSA252)

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 1.13 Artem Cherkasov, Michael Hsing, Roya Zoraghi, Leonard J Foster, Raymond H See, Nikolay Stoynov, Jihong Jiang, Sukhbir Kaur, Tian Lian, Linda Jackson, Huansheng Gong, Rick Swayze, Emily Amandoron, Farhad Hormozdiari, Phuong Dao, Cenk Sahinalp, Osvaldo Santos-Filho, Peter Axerio-Cilies, Kendall Byler, William R McMaster, Robert C Brunham, B Brett Finlay, Neil E Reiner
    Mapping the protein interaction network in methicillin-resistant Staphylococcus aureus.
    J Proteome Res: 2011, 10(3);1139-50
    [PubMed:21166474] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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