PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS10265 [old locus tag: SACOL1963 ]
• pan locus tag^?: SAUPAN004929000
• symbol: SACOL_RS10265
• pan gene symbol^?: putP
• synonym:
• product: sodium:proline symporter

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS10265 [old locus tag: SACOL1963 ]
• symbol: SACOL_RS10265
• product: sodium:proline symporter
• replicon: chromosome
• strand: +
• coordinates: 2023853..2025391
• length: 1539
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (2023853..2025391) NCBI
• BioCyc: SACOL_RS10265 BioCyc
• MicrobesOnline: see SACOL1963

⊟Phenotype

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⊟DNA sequence

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  ATGCTTACAATGGGGACAGCATTAAGTCAACAAGTAGATGCCAATTGGCAAACTTATATT
  ATGATTGCCGTCTACTTCTTGATACTAATCGTTATTGGCTTTTACGGTTACAAGCAAGCA
  ACTGGTAACCTAAGCGAGTACATGTTAGGTGGACGTAGTATTGGACCGTATATTACTGCA
  TTATCAGCTGGAGCTTCAGATATGAGTGGATGGATGATTATGGGGCTACCTGGTTCTGTC
  TATAGCACTGGTCTATCAGCTATGTGGATTACAATCGGTTTAACATTAGGTGCTTATATA
  AATTACTTTGTTGTTGCTCCTAGACTTCGTGTTTATACCGAATTAGCTGGAGATGCAATT
  ACATTACCAGATTTCTTTAAAAATCGTTTAAACGATAAAAATAATGTGTTAAAGATTATT
  TCTGGATTGATTATCGTAGTATTCTTTACATTATATACACATTCTGGTTTCGTATCTGGT
  GGTAAACTATTTGAAAGTGCTTTTGGATTAGATTATCATTTCGGTTTAATATTAGTTGCT
  TTCATTGTCATTTTCTATACTTTCTTTGGTGGATATTTAGCTGTATCAATTACAGATTTC
  TTCCAAGGTGTCATTATGTTAATTGCGATGGTTATGGTCCCTATTGTTGCTATGATGAAT
  TTAAACGGCTGGGGAACGTTTCATGATGTAGCAGCTATGAAACCTACAAATTTAAATTTA
  TTTAAAGGGTTATCATTTATAGGAATTATCTCTCTATTTTCATGGGGATTAGGTTATTTC
  GGTCAACCTCATATCATTGTAAGGTTTATGTCTATTAAATCACACAAGATGCTACCTAAA
  GCTAGACGTTTAGGTATTAGCTGGATGGCTGTTGGTTTATTAGGCGCTGTGGCTGTTGGT
  TTAACAGGTATTGCATTCGTACCTGCTTATCATATTAAACTAGAAGATCCTGAGACATTA
  TTCATCGTGATGAGTCAAGTACTCTTCCATCCTCTTGTAGGTGGTTTCTTACTTGCTGCG
  ATTCTAGCTGCAATTATGAGCACGATTTCTTCACAATTACTTGTAACATCTAGTTCACTA
  ACGGAAGACTTTTATAAATTAATTCGTGGTGAAGAAAAAGCTAAAACGCACCAAAAAGAA
  TTTGTTATGATTGGAAGATTATCTGTATTAGTTGTAGCAATTGTTGCCATCGCGATTGCA
  TGGAATCCAAACGACACAATTCTAAACTTAGTAGGTAACGCTTGGGCCGGATTTGGTGCA
  TCGTTCAGTCCACTTGTGCTATTTGCACTTTACTGGAAAGGTTTGACACGTGCCGGTGCT
  GTAAGTGGAATGGTTTCAGGTGCCTTAGTCGTTATCGTTTGGATTGCGTGGATTAAACCA
  TTGGCACATATCAACGAAATATTCGGCTTATATGAAATTATTCCTGGATTTATTGTAAGT
  GTAATCGTTACATATGTTGTAAGTAAACTTACTAAAAAACCTGGTGCATTTGTTGAAACT
  GACTTAAACAAAGTTCGTGACATCGTTAGAGAAAAATAA
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⊟Protein

⊟General

• locus tag: SACOL_RS10265 [old locus tag: SACOL1963 ]
• symbol: SACOL_RS10265
• description: sodium:proline symporter
• length: 512
• theoretical pI: 9.68032
• theoretical MW: 55975.1
• GRAVY: 0.778711

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Amino acids, peptides and amines sodium/proline symporter (TIGR02121; HMM-score: 768.8)
  and 5 more
  Metabolism Transport and binding proteins Cations and iron carrying compounds transporter, solute:sodium symporter (SSS) family (TIGR00813; HMM-score: 297.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A sodium/pantothenate symporter (TIGR02119; HMM-score: 202.8)
  Metabolism Transport and binding proteins Other sodium/pantothenate symporter (TIGR02119; HMM-score: 202.8)
  probable sodium:solute symporter, VC_2705 subfamily (TIGR03648; HMM-score: 121.4)
  Metabolism Transport and binding proteins Carbohydrates, organic alcohols, and acids cation/acetate symporter ActP (TIGR02711; HMM-score: 107)
• TheSEED: see SACOL1963
• PFAM:
  APC (CL0062) SSF; Sodium:solute symporter family (PF00474; HMM-score: 329)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 13
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.014411
  • TAT(Tat/SPI): 0.00039
  • LIPO(Sec/SPII): 0.001527
• predicted transmembrane helices (TMHMM): 13

⊟Accession numbers

• GI: 446879764 NCBI
• RefSeq: WP_000957020 NCBI
• UniProt: see SACOL1963

⊟Protein sequence

• MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNLSEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINYFVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSGGKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAMMNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHKMLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVGGFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVVAIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALVVIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDIVREK

⊟Experimental data

• experimentally validated: see SACOL1963
• protein localization: see SACOL1963
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CcpA see SACOL1963

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications