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NCBI: 26-AUG-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus N315
- locus tag: SA1718 [new locus tag: SA_RS09865 ]
- pan locus tag?: SAUPAN004929000
- symbol: putP
- pan gene symbol?: putP
- synonym:
- product: high affinity proline permease
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SA1718 [new locus tag: SA_RS09865 ]
- symbol: putP
- product: high affinity proline permease
- replicon: chromosome
- strand: +
- coordinates: 1965656..1967194
- length: 1539
- essential: no DEG other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 1124602 NCBI
- RefSeq: NP_375009 NCBI
- BioCyc: see SA_RS09865
- MicrobesOnline: 104035 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1501ATGCTTACAATGGGGACAGCATTAAGTCAACAAGTAGATGCCAATTGGCAAACTTATATT
ATGATTGCCGTCTACTTCTTGATACTAATCGTTATTGGCTTTTACGGTTACAAGCAAGCA
ACTGGTAACCTAAGCGAGTACATGTTAGGTGGACGTAGTATTGGACCGTATATTACTGCA
TTATCAGCTGGAGCTTCAGATATGAGTGGATGGATGATTATGGGGCTACCTGGTTCTGTC
TATAGCACTGGTCTATCAGCTATGTGGATTACAATCGGTTTAACATTAGGTGCTTATATA
AATTACTTTGTTGTTGCTCCTAGACTTCGTGTTTATACCGAATTAGCTGGAGATGCAATT
ACATTACCAGATTTCTTTAAAAATCGTTTAAACGATAAAAATAATGTGTTAAAGATTATT
TCTGGATTGATTATCGTAGTATTCTTTACATTATATACACATTCTGGTTTCGTATCTGGT
GGTAAATTATTTGAAAGTGCTTTTGGATTAGATTATCATTTCGGTTTAATATTAGTTGCT
TTCATTGTCATTTTCTATACTTTCTTTGGTGGATATTTAGCTGTATCAATTACAGATTTC
TTCCAAGGTGTCATTATGTTAATTGCGATGGTTATGGTCCCTATTGTTGCTATGATGAAT
TTAAACGGCTGGGGAACGTTTCATGATGTAGCAGCTATGAAACCTACAAATTTAAATTTA
TTTAAAGGGTTATCATTTATAGGAATTATCTCTCTATTTTCATGGGGATTAGGTTATTTC
GGTCAACCTCATATCATTGTAAGGTTTATGTCTATTAAATCACACAAGATGCTACCTAAA
GCTAGACGTTTAGGTATTAGCTGGATGGCTGTTGGTTTATTAGGCGCTGTGGCTGTTGGT
TTAACAGGTATTGCATTCGTACCTGCTTATCATATTAAACTAGAAGATCCTGAGACATTA
TTCATCGTGATGAGTCAAGTACTCTTCCATCCTCTTGTAGGTGGTTTCTTACTTGCTGCG
ATTCTAGCTGCAATTATGAGCACGATTTCTTCACAATTACTTGTAACATCTAGTTCACTA
ACGGAAGACTTTTATAAATTAATTCGTGGTGAAGAAAAAGCTAAAACGCACCAAAAAGAA
TTTGTTATGATTGGAAGATTATCTGTATTAGTTGTAGCAATTGTTGCCATCGCGATTGCA
TGGAATCCAAACGACACAATTCTAAACTTAGTAGGTAACGCTTGGGCCGGATTTGGTGCA
TCGTTCAGTCCACTTGTGCTATTTGCACTTTACTGGAAAGGTTTGACACGTGCCGGTGCT
GTAAGTGGAATGGTTTCAGGTGCCTTAGTCGTTATCGTTTGGATTGCGTGGATTAAACCA
TTGGCACATATCAACGAAATATTCGGCTTATATGAAATTATTCCTGGATTTATTGTAAGT
GTAATCGTTACATATGTTGTAAGTAAACTTACTAAAAAACCTGGTGCATTTGTTGAAACT
GACTTAAACAAAGTTCGTGACATCGTTAGAGAAAAATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SA1718 [new locus tag: SA_RS09865 ]
- symbol: PutP
- description: high affinity proline permease
- length: 512
- theoretical pI: 9.68032
- theoretical MW: 55975.1
- GRAVY: 0.778711
⊟Function[edit | edit source]
- TIGRFAM: Transport and binding proteins Amino acids, peptides and amines sodium/proline symporter (TIGR02121; HMM-score: 768.8)and 5 moreTransport and binding proteins Cations and iron carrying compounds transporter, solute:sodium symporter (SSS) family (TIGR00813; HMM-score: 297.2)Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A sodium/pantothenate symporter (TIGR02119; HMM-score: 202.8)Transport and binding proteins Other sodium/pantothenate symporter (TIGR02119; HMM-score: 202.8)probable sodium:solute symporter, VC_2705 subfamily (TIGR03648; HMM-score: 121.4)Transport and binding proteins Carbohydrates, organic alcohols, and acids cation/acetate symporter ActP (TIGR02711; HMM-score: 107)
- TheSEED :
- Pantothenate:Na+ symporter (TC 2.A.21.1.1)
- PFAM: APC (CL0062) SSF; Sodium:solute symporter family (PF00474; HMM-score: 329)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic Membrane
- Cytoplasmic Score: 0
- Cytoplasmic Membrane Score: 10
- Cellwall Score: 0
- Extracellular Score: 0
- Internal Helices: 13
- LocateP: Multi-transmembrane
- Prediction by SwissProt Classification: Membrane
- Pathway Prediction: Sec-(SPI)
- Intracellular possibility: 0.17
- Signal peptide possibility: -1
- N-terminally Anchored Score: -1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.014411
- TAT(Tat/SPI): 0.00039
- LIPO(Sec/SPII): 0.001527
- predicted transmembrane helices (TMHMM): 13
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNLSEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINYFVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVVFFTLYTHSGFVSGGKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAMMNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHKMLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVGGFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLVVAIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALVVIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDIVREK
⊟Experimental data[edit | edit source]
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
- MicrobesOnline: no polycistronic organisation predicted
⊟Regulation[edit | edit source]
- regulator: CcpA regulon
CcpA (TF) important in Carbon catabolism; RegPrecise
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
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