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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS08330 [old locus tag: SACOL1634 ]
  • pan locus tag?: SAUPAN004160000
  • symbol: SACOL_RS08330
  • pan gene symbol?: rsmE
  • synonym:
  • product: 16S rRNA (uracil(1498)-N(3))-methyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS08330 [old locus tag: SACOL1634 ]
  • symbol: SACOL_RS08330
  • product: 16S rRNA (uracil(1498)-N(3))-methyltransferase
  • replicon: chromosome
  • strand: -
  • coordinates: 1663603..1664355
  • length: 753
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (1663603..1664355) NCBI
  • BioCyc: SACOL_RS08330 BioCyc
  • MicrobesOnline: see SACOL1634

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    GTGCAACGTTATTTCATAGACCAAAACGCTGATGTAAGTCAGCGTTTTTTTATTACAAAA
    AAAGAAGATATTCATCATATAACAAATGTAATGAGATATGATGTTGGTAGTAAAATTATT
    TTAACTTTTAATGATCAAACAGTTTATTTATGTGAAATTGCTGACATAACAAGTGATAAA
    ATTGAAGTTGATTTAAAAGAAAAACAAAATATTAATACAGAATTGCCAGTTGATGTTACG
    ATTTGCAGTGGACTAATCAAAGCTGACAAATATGAGTGGTTGCTACAAAAAGCTACTGAA
    TTGGGTGCTTCATCATTTATAGCTGTGAGCATGGAACGTTCAATTGTTAAATTAAACGAG
    GCAAAAGTTGCCAAAAAGATAGAGCGTTGGCAAAAAATAATTAAAGAAGCTGCTGAACAA
    AGCTATCGTTTAGTGATTCCATCTATTCAATTCGAGTCGAATTTAAAATTAATTTGTGAT
    ACTATTGATAATTATGACTATATTCTTATTGCATATGAAGAGGAAGCAAAAGATGGTGAG
    TTAAGCAATTTCAAGCAAACTTTACAACAATTCAATGCTCAGGATAAAGTGTTGATGATT
    TTTGGTCCTGAGGGTGGTTTGTCAGAAAATGAAATTTCATTATTTAGTAATACTAGTACA
    GTTGTTGGTCTTGGGCCGAGAATTTTACGGGCAGAAACTGCGCCATTGTATGCGTTAAGT
    GCAATAAGTTATGAAAAAGAATTAATGGGGTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    753

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS08330 [old locus tag: SACOL1634 ]
  • symbol: SACOL_RS08330
  • description: 16S rRNA (uracil(1498)-N(3))-methyltransferase
  • length: 250
  • theoretical pI: 4.60587
  • theoretical MW: 28469.4
  • GRAVY: -0.1916

⊟Function[edit | edit source]

  • ⊞reaction:
    EC 2.1.1.193?  ExPASy
    16S rRNA (uracil1498-N3)-methyltransferase S-adenosyl-L-methionine + uracil1498 in 16S rRNA = S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification RNA methyltransferase, RsmE family (TIGR00046; EC 2.1.1.-; HMM-score: 204.7)
  • TheSEED: see SACOL1634
  • ⊞PFAM:
    SPOUT (CL0098) Methyltrans_RNA; RNA methyltransferase domain (PF04452; HMM-score: 185)
    and 3 more
    PUA (CL0178) PUA_4; RNA methyltransferase PUA domain (PF20260; HMM-score: 52.4)
    SPOUT (CL0098) SpoU_methylase; SpoU rRNA Methylase family (PF00588; HMM-score: 13.6)
    bBprotInhib (CL0354) Staphostatin_A; Staphostatin A (PF09022; HMM-score: 11.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • ⊞PSORTb: unknown (no significant prediction)
    • Cytoplasmic Score: 2.5
    • Cytoplasmic Membrane Score: 2.5
    • Cellwall Score: 2.5
    • Extracellular Score: 2.5
    • Internal Helices: 0
  • ⊞DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9875
    • Cytoplasmic Membrane Score: 0.003
    • Cell wall & surface Score: 0.0003
    • Extracellular Score: 0.0092
  • LocateP:
  • ⊞SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.001491
    • TAT(Tat/SPI): 0.000117
    • LIPO(Sec/SPII): 0.000288
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFNDQTVYLCEIADITSDKIEVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELGASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICDTIDNYDYILIAYEEEAKDGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLFSNTSTVVGLGPRILRAETAPLYALSAISYEKELMG

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL1634
  • protein localization: see SACOL1634
  • quantitative data / protein copy number per cell: see SACOL1634
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊞Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊞Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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