PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS07890 [old locus tag: SACOL1549 ]
• pan locus tag^?: SAUPAN004039000
• symbol: SACOL_RS07890
• pan gene symbol^?: zwf
• synonym:
• product: glucose-6-phosphate dehydrogenase

⊟Additional information (user-provided)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS07890 [old locus tag: SACOL1549 ]
• symbol: SACOL_RS07890
• product: glucose-6-phosphate dehydrogenase
• replicon: chromosome
• strand: +
• coordinates: 1584167..1585651
• length: 1485
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1584167..1585651) NCBI
• BioCyc: SACOL_RS07890 BioCyc
• MicrobesOnline: see SACOL1549

⊟Phenotype

⊟Additional information (user-provided)

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  
  TTGAGTACTAAAAACAAACACATCCCATGTTTAATCACAATCTTTGGTGCAACTGGTGAC
  TTAAGCCATCGTAAGTTGTTTCCATCAATATTCCATCTCTACCAACAAGACAATTTAGAT
  GAACATATTGCCATCATCGGTATTGGACGTCGTGACATTACTAATGATGATTTCCGTAAT
  CAAGTAAAATCATCAATTCAAAAGCACGTAAAAGATACAAACAAAATTGACGCGTTTATG
  GAACATGTCTTCTATCATAGACATGATGTTAGTAATGAAGAAAGCTATCAAGAATTACTA
  GATTTTAGTAATGAATTAGATAGCCAATTTGAATTAAAAGGTAATCGACTATTCTATTTA
  GCAATGGCACCACAATTCTTTGGCGTTATTTCTGATTATCTAAAATCTTCTGGTCTTACT
  GATACAAAAGGATTTAAACGCCTTGTTATCGAAAAACCATTCGGTAGTGATTTAAAATCA
  GCCGAAGCATTAAACAATCAAATTCGTAAATCATTTAAAGAAGAAGAAATTTATCGTATT
  GACCACTATTTAGGAAAAGACATGGTTCAAAATATCGAGGTATTACGTTTTGCGAATGCG
  ATGTTTGAACCATTATGGAATAACAAATATATTTCAAACATCCAAGTTACATCTTCTGAA
  ATACTAGGTGTTGAAGATCGTGGTGGTTATTATGAATCAAGTGGCGCGCTAAAAGATATG
  GTGCAAAACCACATGTTACAAATGGTTGCATTATTAGCTATGGAAGCACCTATTAGTTTA
  AATAGTGAAGATATCCGTGCTGAGAAAGTAAAAGTACTTAAATCACTGCGTCATTTCCAA
  TCTGAAGATGTTAAAAAGAACTTTGTTCGTGGTCAATATGGCGAAGGCTATATCGATGGT
  AAACAAGTTAAAGCATACCGTGATGAAGATCGCGTTGCAGATGACTCTAACACACCTACC
  TTTGTTTCAGGTAAATTAACAATTGATAACTTTAGATGGGCTGGTGTACCATTCTATATT
  CGTACTGGTAAACGTATGAAATCTAAAACAATTCAAGTTGTCGTTGAATTTAAAGAAGTA
  CCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAAT
  ATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATC
  GAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTA
  GATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCAC
  TGGGAAGAATTAAAATCAACATGGAAATTTGTTGATGCAATTCAAGATGAATGGAATATG
  GTTGATCCAGAATTCCCTAACTATGAATCAGGTACTAATGGTCCATTAGAAAGTGATTTA
  CTACTTGCTCGTGATGGTAACCATTGGTGGGACGATATTCAATAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1485
  

⊟Protein

⊟General

• locus tag: SACOL_RS07890 [old locus tag: SACOL1549 ]
• symbol: SACOL_RS07890
• description: glucose-6-phosphate dehydrogenase
• length: 494
• theoretical pI: 5.18811
• theoretical MW: 56964.8
• GRAVY: -0.531377

⊟Function

• reaction:
  EC 1.1.1.49?  ExPASy
  Glucose-6-phosphate dehydrogenase (NADP⁺) D-glucose 6-phosphate + NADP⁺ = 6-phospho-D-glucono-1,5-lactone + NADPH
• TIGRFAM:
  Metabolism Energy metabolism Pentose phosphate pathway glucose-6-phosphate dehydrogenase (TIGR00871; EC 1.1.1.49; HMM-score: 635.3)
• TheSEED: see SACOL1549
• PFAM:
  GADPH_aa-bio_dh (CL0139) G6PD_C; Glucose-6-phosphate dehydrogenase, C-terminal domain (PF02781; HMM-score: 396)
  and 2 more
  NADP_Rossmann (CL0063) G6PD_N; Glucose-6-phosphate dehydrogenase, NAD binding domain (PF00479; HMM-score: 217)
  no clan defined DUF3775; Protein of unknown function (DUF3775) (PF12616; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9181
  • Cytoplasmic Membrane Score: 0.0221
  • Cell wall & surface Score: 0.0059
  • Extracellular Score: 0.0539
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.023101
  • TAT(Tat/SPI): 0.000621
  • LIPO(Sec/SPII): 0.034757
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446027215 NCBI
• RefSeq: WP_000105070 NCBI
• UniProt: see SACOL1549

⊟Additional information (user-provided)

⊟Protein sequence

• MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIAIIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDFSNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKSAEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTSSEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSLRHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWAGVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLNAKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKFVDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ

⊟Experimental data

• experimentally validated: see SACOL1549
• protein localization: see SACOL1549
• quantitative data / protein copy number per cell: see SACOL1549
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Additional information (user-provided)

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL1549

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Additional information (user-provided)

⊟Other information (user-provided)

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications