PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_1412 [new locus tag: NWMN_RS07970 ]
• pan locus tag^?: SAUPAN004039000
• symbol: zwf
• pan gene symbol^?: zwf
• synonym:
• product: glucose-6-phosphate 1-dehydrogenase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_1412 [new locus tag: NWMN_RS07970 ]
• symbol: zwf
• product: glucose-6-phosphate 1-dehydrogenase
• replicon: chromosome
• strand: +
• coordinates: 1580347..1581831
• length: 1485
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5330840 NCBI
• RefSeq: YP_001332446 NCBI
• BioCyc:
• MicrobesOnline: 3706964 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  TTGAGTACTAAAAACAAACACATCCCATGTTTAATCACAATCTTTGGTGCAACTGGTGAC
  TTAAGCCATCGTAAGTTGTTTCCATCAATATTCCATCTCTACCAACAAGACAATTTAGAT
  GAACATATTGCCATCATCGGTATTGGACGTCGTGACATTACTAATGATGATTTCCGTAAT
  CAAGTAAAATCATCAATTCAAAAGCACGTAAAAGATACAAACAAAATTGACGCGTTTATG
  GAACATGTCTTCTATCATAGACATGATGTTAGTAATGAAGAAAGCTATCAAGAATTACTA
  GATTTTAGTAATGAATTAGATAGCCAATTTGAATTAAAAGGTAATCGACTATTCTATTTA
  GCAATGGCACCACAATTCTTTGGCGTTATTTCTGATTATCTAAAATCTTCTGGTCTTACT
  GATACAAAAGGATTTAAACGCCTTGTTATCGAAAAACCATTCGGTAGTGATTTAAAATCA
  GCCGAAGCATTAAACAATCAAATTCGTAAATCATTTAAAGAAGAAGAAATTTATCGTATT
  GACCACTATTTAGGAAAAGACATGGTTCAAAATATCGAGGTATTACGTTTTGCGAATGCG
  ATGTTTGAACCATTATGGAATAACAAATATATTTCAAACATCCAAGTTACATCTTCTGAA
  ATACTAGGTGTTGAAGATCGTGGTGGTTATTATGAATCAAGTGGCGCGCTAAAAGATATG
  GTGCAAAACCACATGTTACAAATGGTTGCATTATTAGCTATGGAAGCACCTATTAGTTTA
  AATAGTGAAGATATCCGTGCTGAGAAAGTAAAAGTACTTAAATCACTGCGTCATTTCCAA
  TCTGAAGATGTTAAAAAGAACTTTGTTCGTGGTCAATATGGCGAAGGCTATATCGATGGT
  AAACAAGTTAAAGCATACCGTGATGAAGATCGCGTTGCAGATGACTCTAACACACCTACC
  TTTGTTTCAGGTAAATTAACAATTGATAACTTTAGATGGGCTGGTGTACCATTCTATATT
  CGTACTGGTAAACGTATGAAATCTAAAACAATTCAAGTTGTCGTTGAATTTAAAGAAGTA
  CCAATGAACTTATACTATGAAACTGATAAACTGTTAGATTCAAACCTATTAGTAATCAAT
  ATCCAACCTAATGAAGGTGTATCTTTACATCTAAATGCTAAGAAAAATACACAAGGTATC
  GAAACAGAACCTGTCCAATTGTCTTACTCAATGAGCGCTCAAGATAAAATGAATACTGTA
  GATGCATATGAAAATCTATTATTTGATTGTCTTAAAGGTGATGCCACTAACTTCACGCAC
  TGGGAAGAATTAAAATCAACATGGAAATTTGTTGATGCAATTCAAGATGAATGGAATATG
  GTTGATCCAGAATTCCCTAACTATGAATCAGGTACTAATGGTCCATTAGAAAGTGATTTA
  CTACTTGCTCGTGATGGTAACCATTGGTGGGACGATATTCAATAA
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⊟Protein

⊟General

• locus tag: NWMN_1412 [new locus tag: NWMN_RS07970 ]
• symbol: Zwf
• description: glucose-6-phosphate 1-dehydrogenase
• length: 494
• theoretical pI: 5.18811
• theoretical MW: 56964.8
• GRAVY: -0.531377

⊟Function

• reaction:
  EC 1.1.1.49?  ExPASy
  Glucose-6-phosphate dehydrogenase (NADP⁺) D-glucose 6-phosphate + NADP⁺ = 6-phospho-D-glucono-1,5-lactone + NADPH
• TIGRFAM:
  Metabolism Energy metabolism Pentose phosphate pathway glucose-6-phosphate dehydrogenase (TIGR00871; EC 1.1.1.49; HMM-score: 635.3)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined G6PD_C; Glucose-6-phosphate dehydrogenase, C-terminal domain (PF02781; HMM-score: 378.2)
  and 1 more
  NADP_Rossmann (CL0063) G6PD_N; Glucose-6-phosphate dehydrogenase, NAD binding domain (PF00479; HMM-score: 207.7)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.023101
  • TAT(Tat/SPI): 0.000621
  • LIPO(Sec/SPII): 0.034757
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221624 NCBI
• RefSeq: YP_001332446 NCBI
• UniProt: A0A0H3KGM7 UniProt

⊟Protein sequence

• MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIAIIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDFSNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKSAEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTSSEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSLRHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWAGVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLNAKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKFVDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: no polycistronic organisation predicted

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications