PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

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⊟Summary

• organism: Staphylococcus aureus COL
locus tag: SACOL_RS07560 [old locus tag: SACOL1483 ]
pan locus tag^?: SAUPAN003900000
symbol: SACOL_RS07560
pan gene symbol^?: —
• synonym:
• product: RNA methyltransferase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS07560 [old locus tag: SACOL1483 ]
• symbol: SACOL_RS07560
• product: RNA methyltransferase
• replicon: chromosome
• strand: -
• coordinates: 1523994..1525139
• length: 1146
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1523994..1525139) NCBI
• BioCyc: SACOL_RS07560 BioCyc
• MicrobesOnline: see SACOL1483

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
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  301
  361
  421
  481
  541
  601
  661
  721
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  841
  901
  961
  1021
  1081
  1141
  
  ATGTTTCAATTACTTGCAGTTTGTCCGATGGGATTAGAAGCTGTTGTTGCTAGGGAAATT
  CAAGAATTAGGCTATGAAACAAATGTTGAAAATGGTCGTATATTTTTTGAAGGAGACGCA
  AGTGCAATTGTAAAGGCAAATTTATGGTTGCGCACAGCAGACCGAATCAAAATTGTTGTT
  GGACGTTTTAACGCAACAACGTTTGACGAATTATTCGAACAAACCAAAGCGCTCCCTTGG
  GAATCTATAATTGATAAAGAGGGTAACTTCCCAGTTCAAGGTAGAAGCGTTAAATCAACA
  CTACATAGTGTGCCAGATTGTCAGGCGATAACTAAGAAAGCTATTGTTGAACGATTACGT
  CGCGCATATAACGAAAAAGGTTGGTTAAATGAATCAGGTGCCAAATACCCTGTTGAAGTT
  GCCATTTTAAAAGATAATGTATTATTGACTATCGACACATCAGGTTCTGGTTTGAACAGA
  CGTGGTTATAGATTAGCACAAGGTGAAGCACCAATTAAAGAAACGTTGGCAGCAAGTTTA
  ATCCGTCTTGCAAACTGGAAAGGTGATACACCTTTAATTGATCCATTTTGCGGTTCGGGT
  ACAATAGCTATAGAAGCTTGTTTAATTGCTCAAAATATCGCACCTGGTTTTAATCGCGAG
  TTCGTATCAGAGCAATGGAACATCATGCCAGCAAATATTTATGATGATTACCGTGATGAA
  GCGGATAAGATGGCTGATTATGATAAAGAAATCGAAGTATATGCTTCTGATATCGATCCA
  GAAATGGTAGAGATTGCTAAGCGTAACGCTGAAGAAGTTGGGTTGTCTGATATTATTAAA
  TTTAGTGTAAAAGATGTCAATACATTAACAATTGATACAGAAGAACCGGTGGCGTTAATT
  GGAAATCCTCCATATGGTGAACGTATTGGTGATCGTGAAGAAGTTGAAGAAATGTACCGT
  TATATTGGTAAACTAATGAAACAACATCCATTTTTATCTACATACATTTTAACAAGTAAT
  AAAGAATTTGAATATTTAGTAGATCGTAAAGCAACAAAGCGACGTAAGTTATTTAATGGA
  TATATAGAATGTACTTATTATCAATACTGGGGTAAAAAGACAGAACGTAAAACTATTGAA
  AACTAA
  60
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  1146
  

⊟Protein

⊟General

• locus tag: SACOL_RS07560 [old locus tag: SACOL1483 ]
• symbol: SACOL_RS07560
• description: RNA methyltransferase
• length: 381
• theoretical pI: 4.78856
• theoretical MW: 43333
• GRAVY: -0.376115

⊟Function

• ⊞TIGRFAM:
  Hypothetical proteins Conserved putative methyltransferase, TIGR01177 family (TIGR01177; HMM-score: 51.9)
  and 10 more
  Genetic information processing Protein fate Protein modification and repair protein-(glutamine-N5) methyltransferase, release factor-specific (TIGR03534; EC 2.1.1.-; HMM-score: 32.6)
  Genetic information processing Protein fate Protein modification and repair methyltransferase, HemK family (TIGR00536; HMM-score: 30.1)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Chlorophyll and bacteriochlorphyll magnesium protoporphyrin O-methyltransferase (TIGR02021; EC 2.1.1.11; HMM-score: 19.8)
  Genetic information processing DNA metabolism Restriction/modification type II restriction m6 adenine DNA methyltransferase, Alw26I/Eco31I/Esp3I family (TIGR02987; EC 2.1.1.72; HMM-score: 19)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific (TIGR03533; EC 2.1.1.-; HMM-score: 19)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin precorrin-6Y C5,15-methyltransferase (decarboxylating), CbiT subunit (TIGR02469; EC 2.1.1.132; HMM-score: 16.2)
  Genetic information processing Protein synthesis tRNA and rRNA base modification 23S rRNA (uracil-5-)-methyltransferase RumA (TIGR00479; EC 2.1.1.-; HMM-score: 15.6)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification putative protein-(glutamine-N5) methyltransferase, unknown substrate-specific (TIGR03704; EC 2.1.1.-; HMM-score: 14.5)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Menaquinone and ubiquinone ubiquinone/menaquinone biosynthesis methyltransferase (TIGR01934; EC 2.1.1.-; HMM-score: 12.4)
  Genetic information processing Protein synthesis Ribosomal proteins: synthesis and modification ribosomal protein L11 methyltransferase (TIGR00406; EC 2.1.1.-; HMM-score: 12.1)
• TheSEED: see SACOL1483
• ⊞PFAM:
  NADP_Rossmann (CL0063) UPF0020; RMKL-like, methyltransferase domain (PF01170; HMM-score: 195.1)
  and 14 more
  no clan defined RlmL_1st; RlmL ferredoxin-like domain (PF22020; HMM-score: 93.2)
  THUMP (CL0747) THUMP; THUMP domain (PF02926; HMM-score: 62.1)
  NADP_Rossmann (CL0063) Methyltransf_31; Methyltransferase domain (PF13847; HMM-score: 25)
  Methyltransf_25; Methyltransferase domain (PF13649; HMM-score: 21.1)
  N6_N4_Mtase; DNA methylase (PF01555; HMM-score: 17.7)
  Methyltransf_11; Methyltransferase domain (PF08241; HMM-score: 17)
  MTS; Methyltransferase small domain (PF05175; HMM-score: 16.5)
  Eco57I; Eco57I restriction-modification methylase (PF07669; HMM-score: 16.3)
  Cons_hypoth95; Conserved hypothetical protein 95 (PF03602; HMM-score: 15.4)
  Methyltransf_15; RNA cap guanine-N2 methyltransferase (PF09445; HMM-score: 15.4)
  TRM5-TYW2_MTfase; TRM5/TYW2 methyltransferase domain (PF02475; HMM-score: 14)
  PrmA; Ribosomal protein L11 methyltransferase (PrmA) (PF06325; HMM-score: 12.3)
  AviRa; RRNA methyltransferase AviRa (PF11599; HMM-score: 12.3)
  TRM; N2,N2-dimethylguanosine tRNA methyltransferase (PF02005; HMM-score: 11.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• ⊞PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• ⊞DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9886
  • Cytoplasmic Membrane Score: 0.0051
  • Cell wall & surface Score: 0.0006
  • Extracellular Score: 0.0057
• LocateP:
• ⊞SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.047727
  • TAT(Tat/SPI): 0.000694
  • LIPO(Sec/SPII): 0.008568
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446409295 NCBI
• RefSeq: WP_000487150 NCBI
• UniProt: see SACOL1483

⊟Protein sequence

• MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIVKANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLHSVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNRRGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFNREFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLSDIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLSTYILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN

⊟Experimental data

• experimentally validated: see SACOL1483
• protein localization: see SACOL1483
• quantitative data / protein copy number per cell: see SACOL1483
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL1483

⊟Protein stability

• half-life: no data available

⊞Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊞Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications