PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS06540 [old locus tag: SACOL1281 ]
• pan locus tag^?: SAUPAN003563000
• symbol: SACOL_RS06540
• pan gene symbol^?: rseP
• synonym: eep
• product: RIP metalloprotease RseP

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS06540 [old locus tag: SACOL1281 ]
• symbol: SACOL_RS06540
• product: RIP metalloprotease RseP
• replicon: chromosome
• strand: +
• coordinates: 1290026..1291312
• length: 1287
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1290026..1291312) NCBI
• BioCyc: SACOL_RS06540 BioCyc
• MicrobesOnline: see SACOL1281

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  121
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  481
  541
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  661
  721
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  841
  901
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  1021
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  1141
  1201
  1261
  
  GTGAGCTATTTAGTTACAATAATTGCATTTATTATTGTTTTTGGTGTACTAGTAACTGTT
  CATGAATATGGCCATATGTTTTTTGCGAAAAGAGCAGGCATTATGTGTCCAGAATTTGCG
  ATCGGTATGGGGCCAAAAATTTTTAGTTTTAGAAAAAATGAAACACTTTACACTATTAGG
  TTATTGCCTGTTGGTGGATATGTTCGTATGGCAGGAGATGGCTTAGAAGAGCCACCAGTC
  GAGCCCGGTATGAACGTTAAAATTAAACTTAATGAAGAAAATGAAATAACACATATCATA
  TTAGATGATCATCATAAGTTTCAACAAATTGAAGCGATCGAAGTTAAAAAATGTGATTTT
  AAGGATGACTTATTCATAGAAGGTATCACTGCTTATGATAATGAAAGACATCATTTTAAA
  ATTGCTAGAAAGTCTTTCTTTGTTGAAAATGGTAGCTTAGTTCAAATTGCTCCGAGAGAC
  AGACAATTTGCACATAAAAAGCCATGGCCGAAATTTTTAACATTATTTGCGGGACCGTTA
  TTTAACTTTATATTAGCTTTAGTCCTATTTATTGGTCTTGCATATTATCAAGGCACGCCT
  ACGTCTACTGTAGAACAAGTCGCAGATAAGTATCCAGCTCAACAAGCAGGATTACAAAAA
  GGTGATAAGATCGTCCAAATTGGCAAATATAAAATATCTGAATTTGATGATGTTGATAAG
  GCGTTAGATAAAGTTAAAGATAATAAGACGACTGTTAAATTTGAACGTGATGGTAAAACA
  AAGTCAGTTGAATTAACACCTAAAAAGACTGAAAAAAAACTGACTAAAGTAAGTTCAGAG
  ACGAAGTATGTTCTCGGATTCCAACCAGCGAGTGAACATACACTTTTTAAACCAATTGTA
  TTCGGATTTAAAAGCTTTTTAATCGGTAGTACTTATATTTTTACAGCTGTAGTAGGTATG
  TTGGCTAGTATATTTACGGGCGGATTCTCATTTGATATGTTAAATGGTCCGGTTGGTATT
  TATCATAACGTCGACTCAGTTGTTAAAGCGGGTATCATTAGCTTAATTGGTTACACTGCG
  TTATTAAGTGTAAACTTAGGTATTATGAATTTAATTCCTATTCCTGCACTAGACGGTGGT
  CGTATTTTATTTGTTATATATGAAGCGATTTTCAGAAAACCAGTTAATAAAAAAGCGGAA
  ACAACGATTATTGCTATTGGTGCCATTTTCATGGTCGTTATAATGATATTAGTAACGTGG
  AATGATATTCGACGATATTTCTTATAA
  60
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  1287
  

⊟Protein

⊟General

• locus tag: SACOL_RS06540 [old locus tag: SACOL1281 ]
• symbol: SACOL_RS06540
• description: RIP metalloprotease RseP
• length: 428
• theoretical pI: 9.54429
• theoretical MW: 48106.2
• GRAVY: 0.175234

⊟Function

• reaction:
  EC 3.4.24.-?  ExPASy
• TIGRFAM:
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides RIP metalloprotease RseP (TIGR00054; EC 3.4.24.-; HMM-score: 287.4)
  and 7 more
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 37.8)
  Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 37.8)
  Cellular processes Cellular processes Sporulation and germination stage IV sporulation protein B (TIGR02860; EC 3.4.21.116; HMM-score: 29.5)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides C-terminal processing peptidase (TIGR00225; EC 3.4.21.-; HMM-score: 27)
  Genetic information processing Protein fate Protein modification and repair C-terminal processing peptidase (TIGR00225; EC 3.4.21.-; HMM-score: 27)
  putative carboxyl-terminal-processing protease, deltaproteobacterial (TIGR03900; EC 3.4.21.-; HMM-score: 19.2)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type II secretion system protein C (TIGR01713; HMM-score: 15.9)
• TheSEED: see SACOL1281
• PFAM:
  Peptidase_MA (CL0126) Peptidase_M50; Peptidase family M50 (PF02163; HMM-score: 233.6)
  and 6 more
  PDZ-like (CL0466) PDZ_2; PDZ domain (PF13180; HMM-score: 40.5)
  PDZ_6; PDZ domain (PF17820; HMM-score: 31.6)
  PDZ; PDZ domain (PF00595; HMM-score: 22)
  PDZ_Tricorn; Tricorn protease PDZ domain (PF14685; HMM-score: 13.4)
  no clan defined DUF4663; Domain of unknown function (DUF4663) (PF15668; HMM-score: 11.9)
  Yip1 (CL0112) DUF1129; Protein of unknown function (DUF1129) (PF06570; HMM-score: 11.2)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 10
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 5
• DeepLocPro: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 0.9984
  • Cell wall & surface Score: 0
  • Extracellular Score: 0.0016
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.068841
  • TAT(Tat/SPI): 0.002422
  • LIPO(Sec/SPII): 0.004465
• predicted transmembrane helices (TMHMM): 5

⊟Accession numbers

• GI: 446043261 NCBI
• RefSeq: WP_000121116 NCBI
• UniProt: see SACOL1281

⊟Protein sequence

• MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMGPKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILDDHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRDRQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGLQKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTEKKLTKVSSETKYVLGFQPASEHTLFKPIVFGFKSFLIGSTYIFTAVVGMLASIFTGGFSFDMLNGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFRKPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL

⊟Experimental data

• experimentally validated: see SACOL1281
• protein localization: see SACOL1281
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications