PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 10-JUN-2013

⊟Summary

• organism: Staphylococcus aureus USA300_FPR3757
• locus tag: SAUSA300_1155 [new locus tag: SAUSA300_RS06255 ]
• pan locus tag^?: SAUPAN003563000
• symbol: SAUSA300_1155
• pan gene symbol^?: rasP
• synonym:
• product: putative membrane-associated zinc metalloprotease

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SAUSA300_1155 [new locus tag: SAUSA300_RS06255 ]
• symbol: SAUSA300_1155
• product: putative membrane-associated zinc metalloprotease
• replicon: chromosome
• strand: +
• coordinates: 1266324..1267508
• length: 1185
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 3915271 NCBI
• RefSeq: YP_493852 NCBI
• BioCyc: see SAUSA300_RS06255
• MicrobesOnline: 1292670 MicrobesOnline

⊟Phenotype

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⊟DNA sequence

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  ATGTGTCCAGAATTTGCGATCGGTATGGGGCCAAAAATTTTTAGTTTTAGAAAAAATGAA
  ACACTTTACACTATTAGGTTATTGCCTGTTGGTGGATATGTTCGTATGGCAGGAGATGGC
  TTAGAAGAGCCACCAGTCGAGCCCGGTATGAACGTTAAAATTAAACTTAATGAAGAAAAT
  GAAATAACACATATCATATTAGATGATCATCATAAGTTTCAACAAATTGAAGCGATCGAA
  GTTAAAAAATGTGATTTTAAGGATGACTTATTCATAGAAGGTATCACTGCTTATGATAAT
  GAAAGACATCATTTTAAAATTGCTAGAAAGTCTTTCTTTGTTGAAAATGGTAGCTTAGTT
  CAAATTGCTCCGAGAGACAGACAATTTGCACATAAAAAGCCATGGCCGAAATTTTTAACA
  TTATTTGCGGGACCGTTATTTAACTTTATATTAGCTTTAGTCCTATTTATTGGTCTTGCA
  TATTATCAAGGCACGCCTACGTCTACTGTAGAACAAGTCGCAGATAAGTATCCAGCTCAA
  CAAGCAGGATTACAAAAAGGTGATAAGATCGTCCAAATTGGCAAATATAAAATATCTGAA
  TTTGATGATGTTGATAAGGCGTTAGATAAAGTTAAAGATAATAAGACGACTGTTAAATTT
  GAACGTGATGGTAAAACAAAGTCAGTTGAATTAACACCTAAAAAGACTGAAAAAAAACTG
  ACTAAAGTAAGTTCAGAGACGAAGTATGTTCTCGGATTCCAACCAGCGAGTGAACATACA
  CTTTTTAAACCAATTGTATTCGGATTTAAAAGCTTTTTAATCGGTAGTACTTATATTTTT
  ACAGCTGTAGTAGGTATGTTGGCTAGTATATTTACGGGCGGATTCTCATTTGATATGTTA
  AATGGTCCGGTTGGTATTTATCATAACGTCGACTCAGTTGTTAAAGCGGGTATCATTAGC
  TTAATTGGTTACACTGCGTTATTAAGTGTAAACTTAGGTATTATGAATTTAATTCCTATT
  CCTGCACTAGACGGTGGTCGTATTTTATTTGTTATATATGAAGCGATTTTCAGAAAACCA
  GTTAATAAAAAAGCGGAAACAACGATTATTGCTATTGGTGCCATTTTCATGGTCGTTATA
  ATGATATTAGTAACGTGGAATGATATTCGACGATATTTCTTATAA
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⊟Protein

⊟General

• locus tag: SAUSA300_1155 [new locus tag: SAUSA300_RS06255 ]
• symbol: SAUSA300_1155
• description: putative membrane-associated zinc metalloprotease
• length: 394
• theoretical pI: 9.49068
• theoretical MW: 44279.5
• GRAVY: 0.0705584

⊟Function

• reaction:
  EC 3.4.24.-?  ExPASy
• TIGRFAM:
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides RIP metalloprotease RseP (TIGR00054; EC 3.4.24.-; HMM-score: 236.6)
  and 7 more
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 38)
  Genetic information processing Protein fate Protein folding and stabilization peptidase Do (TIGR02037; EC 3.4.21.-; HMM-score: 38)
  Cellular processes Cellular processes Sporulation and germination stage IV sporulation protein B (TIGR02860; EC 3.4.21.116; HMM-score: 29.7)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides C-terminal processing peptidase (TIGR00225; EC 3.4.21.-; HMM-score: 27.2)
  Genetic information processing Protein fate Protein modification and repair C-terminal processing peptidase (TIGR00225; EC 3.4.21.-; HMM-score: 27.2)
  putative carboxyl-terminal-processing protease, deltaproteobacterial (TIGR03900; EC 3.4.21.-; HMM-score: 19.4)
  Genetic information processing Protein fate Protein and peptide secretion and trafficking type II secretion system protein C (TIGR01713; HMM-score: 16)
• TheSEED  :
  • Membrane-associated zinc metalloprotease
  Regulation and Cell signaling Regulation and Cell signaling - no subcategory Sex pheromones in Enterococcus faecalis and other Firmicutes  Membrane-associated zinc metalloprotease
  and 1 more
  Stress Response Periplasmic Stress Periplasmic Stress Response  Membrane-associated zinc metalloprotease
• PFAM:
  Peptidase_MA (CL0126) Peptidase_M50; Peptidase family M50 (PF02163; HMM-score: 207.9)
  and 4 more
  PDZ-like (CL0466) PDZ_2; PDZ domain (PF13180; HMM-score: 44.4)
  PDZ; PDZ domain (Also known as DHR or GLGF) (PF00595; HMM-score: 23.5)
  Tricorn_PDZ; Tricorn protease PDZ domain (PF14685; HMM-score: 13.5)
  Yip1 (CL0112) DUF1129; Protein of unknown function (DUF1129) (PF06570; HMM-score: 10.5)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic Membrane
  • Cytoplasmic Score: 0
  • Cytoplasmic Membrane Score: 9.99
  • Cellwall Score: 0
  • Extracellular Score: 0
  • Internal Helices: 4
• LocateP: Multi-transmembrane
  • Prediction by SwissProt Classification: Membrane
  • Pathway Prediction: Sec-(SPI)
  • Intracellular possibility: 0.17
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.004266
  • TAT(Tat/SPI): 0.000439
  • LIPO(Sec/SPII): 0.00085
• predicted transmembrane helices (TMHMM): 4

⊟Accession numbers

• GI: 87162424 NCBI
• RefSeq: YP_493852 NCBI
• UniProt: A0A0H2XJD2 UniProt

⊟Protein sequence

• MCPEFAIGMGPKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILDDHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRDRQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGLQKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTEKKLTKVSSETKYVLGFQPASEHTLFKPIVFGFKSFLIGSTYIFTAVVGMLASIFTGGFSFDMLNGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFRKPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: SAUSA300_1155 > proS

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications