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NCBI: 02-MAR-2017

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL_RS06200 [old locus tag: SACOL1210 ]
  • pan locus tag?: SAUPAN003478000
  • symbol: SACOL_RS06200
  • pan gene symbol?: pyrR
  • synonym:
  • product: bifunctional pyrimidine operon transcriptional regulator/uracil phosphoribosyltransferase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL_RS06200 [old locus tag: SACOL1210 ]
  • symbol: SACOL_RS06200
  • product: bifunctional pyrimidine operon transcriptional regulator/uracil phosphoribosyltransferase
  • replicon: chromosome
  • strand: +
  • coordinates: 1217368..1217895
  • length: 528
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Location: NC_002951 (1217368..1217895) NCBI
  • BioCyc: SACOL_RS06200 BioCyc
  • MicrobesOnline: see SACOL1210

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGTCTGAACGTATCATAATGGATGATGCCGCAATACAACGTACAGTGACGAGAATCGCT
    CACGAAATTTTGGAGTATAACAAAGGTACTGATAATTTAATTCTTTTAGGTATCAAAACA
    AGAGGTGAATATTTAGCGAATCGTATACAAGATAAAATTCATCAAATTGAGCAACAACGT
    ATACCTACTGGAACAATTGATATTACATACTTTAGAGATGATATAGAGCACATGTCATCA
    CTTACGACAAAAGACGCAATAGACATCGACACAGATATTACAGATAAAGTAGTCATCATT
    ATTGACGATGTGCTGTATACTGGTCGAACGGTTCGTGCTTCACTTGATGCTATTTTGCTA
    AATGCTAGACCTATTAAAATTGGTTTAGCTGCTTTGGTTGATCGAGGACATCGTGAGTTA
    CCAATTCGAGCAGATTTTGTTGGTAAAAATATACCTACTTCTAAAGAGGAAACGGTAAGT
    GTCTATTTAGAAGAAATGGATCAAAGAAATGCAGTTATAATTAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    528

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL_RS06200 [old locus tag: SACOL1210 ]
  • symbol: SACOL_RS06200
  • description: bifunctional pyrimidine operon transcriptional regulator/uracil phosphoribosyltransferase
  • length: 175
  • theoretical pI: 5.08873
  • theoretical MW: 19854.6
  • GRAVY: -0.196

⊟Function[edit | edit source]

  • reaction:
    EC 2.4.2.9?  ExPASy
    Uracil phosphoribosyltransferase UMP + diphosphate = uracil + 5-phospho-alpha-D-ribose 1-diphosphate
  • TIGRFAM:
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides hypoxanthine phosphoribosyltransferase (TIGR01203; EC 2.4.2.8; HMM-score: 44.3)
    and 5 more
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Salvage of nucleosides and nucleotides adenine phosphoribosyltransferase (TIGR01090; EC 2.4.2.7; HMM-score: 17.1)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis ribose-phosphate diphosphokinase (TIGR01251; EC 2.7.6.1; HMM-score: 16.2)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR00336; EC 2.4.2.10; HMM-score: 15.2)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis orotate phosphoribosyltransferase (TIGR01367; EC 2.4.2.10; HMM-score: 13.9)
    Cellular processes Cellular processes DNA transformation comF family protein (TIGR00201; HMM-score: 11.5)
  • TheSEED: see SACOL1210
  • PFAM:
    PRTase-like (CL0533) Pribosyltran; Phosphoribosyl transferase domain (PF00156; HMM-score: 68.4)
    and 2 more
    PRTase_2; Phosphoribosyl transferase (PF15609; HMM-score: 13.6)
    PRTase_3; PRTase ComF-like (PF15610; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP:
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002036
    • TAT(Tat/SPI): 0.000168
    • LIPO(Sec/SPII): 0.000277
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEYLANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIIDDVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVSVYLEEMDQRNAVIIK

⊟Experimental data[edit | edit source]

  • experimentally validated: see SACOL1210
  • protein localization: see SACOL1210
  • quantitative data / protein copy number per cell: see SACOL1210
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

⊟Relevant publications[edit | edit source]

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