PangenomeCOLN315NCTC8325NewmanUSA300_FPR3757JSNZ04-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS05500 [old locus tag: SACOL1078 ]
• pan locus tag^?: SAUPAN003282000
• symbol: SACOL_RS05500
• pan gene symbol^?: purL
• synonym:
• product: phosphoribosylformylglycinamidine synthase subunit PurL

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS05500 [old locus tag: SACOL1078 ]
• symbol: SACOL_RS05500
• product: phosphoribosylformylglycinamidine synthase subunit PurL
• replicon: chromosome
• strand: +
• coordinates: 1086152..1088341
• length: 2190
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1086152..1088341) NCBI
• BioCyc: SACOL_RS05500 BioCyc
• MicrobesOnline: see SACOL1078

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGTCTAAATTTATCGAACCAAGCGTTGAAGAAATTAAACTTGAAAAAGTATATCAAGAT
  ATGGGATTAAGTGATCAAGAATATGAAAAAGTTTGCGATATTTTAGGCAGACAACCTAAC
  TTTACAGAAACAGGTATCTTTTCTGTTATGTGGAGTGAACATTGCTCTTATAAACATTCT
  AAACCGTTTTTAAAGCAATTTCCTACGTCAGGTGACCATGTGCTTATGGGGCCTGGTGAA
  GGTGCAGGGGTAGTCGATATAGGTGATAATCAAGCCGTAGTATTTAAAGTAGAGTCTCAC
  AATCATCCATCAGCAATTGAACCATATCAAGGGGCTGCTACAGGCGTTGGTGGAATCATT
  CGTGACATTGTCTCTATTGGGGCTAGACCTATTAATTTGTTAAACAGTCTTAGATTTGGA
  GAATTAGATAATAAACAAAACCAAAGATTACTTAAAGGTGTTGTAAAGGGTATCGGAGGT
  TATGGTAACTGCATTGGTATTCCAACAACTGCTGGTGAAATCGAATTTGATGAACGTTAT
  GATGGCAATCCACTTGTTAATGCAATGTGTGTTGGTGTTATCAATCACGACATGATTCAA
  AAAGGCACAGCAAAAGGTGTAGGTAATTCGGTCATTTATGTTGGTTTGAAAACTGGTCGA
  GATGGTATTCATGGTGCTACTTTTGCATCTGAAGAATTGACGGAAGAAAGCGAAAGTAAA
  CGACCTTCTGTACAAATCGGTGATCCATTTGTAGGTAAAAAATTAATGGAAGCAACACTT
  GAAGCAATTACATTTGATGAATTAGTTGGTATTCAAGATATGGGTGCTGCTGGTTTAACA
  TCTTCATCGTCTGAAATGGCGGCAAAAGGTGGTAGTGGGTTACATTTGAGATTAGAACAA
  GTGCCAACACGTGAGCCAGGTATTTCTCCTTATGAAATGATGCTTTCAGAAACTCAAGAA
  CGTATGTTACTAGTTGTTGAAAAAGGTACTGAACAAAAATTCTTAGATTTATTTGATAAG
  CACGAATTGGATAGTGCTGTTATAGGTGAAGTTACAGATACAAATCGTTTTGTTTTAACA
  TATGATGACGAAGTTTATGCTGACATTCCAGTTGAACCACTAGCTGATGAAGCACCTGTA
  TATATTTTAGAAGGAGAAGAAAAAGATTATAATACTTCTAAAAATGATTATACACACATC
  GATGTTAAAGATACTTTCTTTAAATTACTTAAGCATCCGACTATAGCATCTAAACACTAT
  TTATATGATCAATACGACCAACAAGTTGGTGCCAATACGATAATTAAGCCAGGACTTCAA
  GCATCGGTAGTACGTGTGGAAGGCACAAATAAGGCAATTGCTTCAACAATTGATGGTGAA
  GCGCGTTATGTATATAACAATCCATATGAAGGTGGAAAGATGGTAGTAGCTGAAGCTTAT
  CGAAATTTAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGT
  TCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAA
  GCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTAATGTATCTTTATATAACGAAACG
  AAAGGTACTTCTATTTTCCCAACACCAGTTGTTGGAATGGTAGGTTTGATTGAAAATGTA
  AATTATTTAAATGATTTTGAACCTCAAGTTGGAGATAAATTATATTTAATCGGTGATACT
  AAGGACGACTTTGGTGGTAGTCAACTTGAAAAGTTAATTTATGGCAAAGTTAATCATGAA
  TTTGAGTCATTAGATTTGAGTTCAGAAGTTGAAAAAGGTGAATCAATCAAGACCGCTATT
  CGTGAAGGACTATTATCACATGTTCAAACAGTTGGTAAAGGTGGCTTACTGATTACCTTA
  GCTAAACTAAGTGCGCATTACGGTTTAGGATTAAAATCTTCAATAGATATAACAAATGCA
  CAATTGTTTAGTGAGACGCAAGGCCGATATGTTGTTTCTGTTAAATCAGGTAAAACTTTA
  AATATTGATAATGCAATAGAAATTGGACTTTTAACAGATAGTGATAATTTCAAGGTAACA
  ACACCATATACAGAGATTAGTGAAAATGTTTCAGATATTAAACAAATATGGGAAGGGGCA
  ATTGCTCAATGTTTAACTACTCAGGATTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS05500 [old locus tag: SACOL1078 ]
• symbol: SACOL_RS05500
• description: phosphoribosylformylglycinamidine synthase subunit PurL
• length: 729
• theoretical pI: 4.50802
• theoretical MW: 79535.2
• GRAVY: -0.253086

⊟Function

• reaction:
  EC 6.3.5.3?  ExPASy
  Phosphoribosylformylglycinamidine synthase ATP + N²-formyl-N¹-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H₂O = ADP + phosphate + 2-(formamido)-N¹-(5-phospho-D-ribosyl)acetamidine + L-glutamate
• TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase II (TIGR01736; EC 6.3.5.3; HMM-score: 1004.7)
  and 8 more
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase (TIGR01857; EC 6.3.5.3; HMM-score: 166.4)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase (TIGR01735; EC 6.3.5.3; HMM-score: 139)
  Metabolism Energy metabolism Methanogenesis putative methanogenesis marker protein 2 (TIGR03267; HMM-score: 64.6)
  hydrogenase expression/formation protein HypE (TIGR02124; HMM-score: 41.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate kinase (TIGR01379; EC 2.7.4.16; HMM-score: 37.9)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine cyclo-ligase (TIGR00878; EC 6.3.3.1; HMM-score: 37.1)
  Unknown function Enzymes of unknown specificity AIR synthase-related protein, sll0787 family (TIGR04049; HMM-score: 35.9)
  herpesvirus tegument protein/v-FGAM-synthase (TIGR01739; HMM-score: 12.6)
• TheSEED: see SACOL1078
• PFAM:
  PurM_N-like (CL0804) AIRS; AIR synthase related protein, N-terminal domain (PF00586; HMM-score: 173.3)
  no clan defined AIRS_C; AIR synthase related protein, C-terminal domain (PF02769; HMM-score: 153)
  and 3 more
  FGAR-AT_linker; Formylglycinamide ribonucleotide amidotransferase linker domain (PF18072; HMM-score: 65.3)
  EIF3E_C; Eukaryotic translation initiation factor 3 subunit E, C-terminal (PF21357; HMM-score: 11.7)
  Asp-glut_race (CL0399) AroM; AroM protein (PF07302; HMM-score: 11.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.8876
  • Cytoplasmic Membrane Score: 0.0614
  • Cell wall & surface Score: 0.0014
  • Extracellular Score: 0.0496
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.011831
  • TAT(Tat/SPI): 0.001013
  • LIPO(Sec/SPII): 0.00266
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 445954872 NCBI
• RefSeq: WP_000032727 NCBI
• UniProt: see SACOL1078

⊟Protein sequence

• MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTETGIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNHPSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGGYGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKTGRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGAAGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKFLDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNTSKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTNKAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIYQQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDFEPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGLLSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNIDNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD

⊟Experimental data

• experimentally validated: see SACOL1078
• protein localization: see SACOL1078
• quantitative data / protein copy number per cell: see SACOL1078
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for JSNZ, N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You can add further information about the gene and protein here. [edit]

⊟Literature

⊟References

⊟Relevant publications