PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 06-JUL-2013

⊟Summary

• organism: Staphylococcus aureus Newman
• locus tag: NWMN_0937 [new locus tag: NWMN_RS05250 ]
• pan locus tag^?: SAUPAN003282000
• symbol: purL
• pan gene symbol^?: purL
• synonym:
• product: phosphoribosylformylglycinamidine synthase II

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: NWMN_0937 [new locus tag: NWMN_RS05250 ]
• symbol: purL
• product: phosphoribosylformylglycinamidine synthase II
• replicon: chromosome
• strand: +
• coordinates: 1039325..1041514
• length: 2190
• essential: unknown other strains

⊟Accession numbers

• Gene ID: 5332311 NCBI
• RefSeq: YP_001331971 NCBI
• BioCyc:
• MicrobesOnline: 3706488 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  961
  1021
  1081
  1141
  1201
  1261
  1321
  1381
  1441
  1501
  1561
  1621
  1681
  1741
  1801
  1861
  1921
  1981
  2041
  2101
  2161
  
  ATGTCTAAATTTATCGAACCAAGCGTTGAAGAAATTAAACTTGAAAAAGTATATCAAGAT
  ATGGGATTAAGTGATCAAGAATATGAAAAAGTTTGCGATATTTTAGGCAGACAACCTAAC
  TTTACAGAAACAGGTATCTTTTCTGTTATGTGGAGTGAACATTGCTCTTATAAACATTCT
  AAACCGTTTTTAAAGCAATTTCCTACGTCAGGTGACCATGTGCTTATGGGGCCTGGTGAA
  GGTGCAGGGGTAGTCGATATAGGTGATAATCAAGCCGTAGTATTTAAAGTAGAGTCTCAC
  AATCATCCATCAGCAATTGAACCATATCAAGGGGCTGCTACAGGCGTTGGTGGAATCATT
  CGTGACATTGTCTCTATTGGGGCTAGACCTATTAATTTGTTAAACAGTCTTAGATTTGGA
  GAATTAGATAATAAACAAAACCAAAGATTACTTAAAGGTGTTGTAAAGGGTATCGGAGGT
  TATGGTAACTGCATTGGTATTCCAACAACTGCTGGTGAAATCGAATTTGATGAACGTTAT
  GATGGCAATCCACTTGTTAATGCAATGTGTGTTGGTGTTATCAATCACGACATGATTCAA
  AAAGGCACAGCAAAAGGTGTAGGTAATTCGGTCATTTATGTTGGTTTGAAAACTGGTCGA
  GATGGTATTCATGGTGCTACTTTTGCATCTGAAGAATTGACGGAAGAAAGCGAAAGTAAA
  CGACCTTCTGTACAAATCGGTGATCCATTTGTAGGTAAAAAATTAATGGAAGCAACACTT
  GAAGCAATTACATTTGATGAATTAGTTGGTATTCAAGATATGGGTGCTGCTGGTTTAACA
  TCTTCATCGTCTGAAATGGCGGCAAAAGGTGGTAGTGGGTTACATTTGAGATTAGAACAA
  GTGCCAACACGTGAGCCAGGTATTTCTCCTTATGAAATGATGCTTTCAGAAACTCAAGAA
  CGTATGTTACTAGTTGTTGAAAAAGGTACTGAACAAAAATTCTTAGATTTATTTGATAAG
  CACGAATTGGATAGTGCTGTTATAGGTGAAGTTACAGATACAAATCGTTTTGTTTTAACA
  TATGATGACGAAGTTTATGCTGACATTCCAGTTGAACCACTAGCTGATGAAGCACCTGTA
  TATATTTTAGAAGGAGAAGAAAAAGATTATAATACTTCTAAAAATGATTATACACACATC
  GATGTTAAAGATACTTTCTTTAAATTACTTAAGCATCCGACTATAGCATCTAAACACTAT
  TTATATGATCAATACGACCAACAAGTTGGTGCCAATACGATAATTAAGCCAGGACTTCAA
  GCATCGGTAGTACGTGTGGAAGGCACAAATAAGGCAATTGCTTCAACAATTGATGGTGAA
  GCGCGTTATGTATATAACAATCCATATGAAGGTGGAAAGATGGTAGTAGCTGAAGCTTAT
  CGAAATTTAATTGCCGTGGGTGCAACACCATTAGCAATGACAGATTGTTTAAATTATGGT
  TCTCCTGAAAAGAAAGAAATCTATCAACAGTTGATAGATTCAACGAAAGGTATGGCAGAA
  GCATGCGACATTCTTAAGACACCAGTAGTTTCTGGTAATGTATCTTTATATAACGAAACG
  AAAGGTACTTCTATTTTCCCAACACCAGTTGTTGGAATGGTAGGTTTGATTGAAAATGTA
  AATTATTTAAATGATTTTGAACCTCAAGTTGGAGATAAATTATATTTAATCGGTGATACT
  AAGGACGACTTTGGTGGTAGTCAACTTGAAAAGTTAATTTATGGCAAAGTTAATCATGAA
  TTTGAGTCATTAGATTTGAGTTCAGAAGTTGAAAAAGGTGAATCAATCAAGACCGCTATT
  CGTGAAGGACTATTATCACATGTTCAAACAGTTGGTAAAGGTGGCTTACTGATTACCTTA
  GCTAAACTAAGTGCGCATTACGGTTTAGGATTAAAATCTTCAATAGATATAACAAATGCA
  CAATTGTTTAGTGAGACGCAAGGCCGATATGTTGTTTCTGTTAAATCAGGTAAAACTTTA
  AATATTGATAATGCAATAGAAATTGGACTTTTAACAGATAGTGATAATTTCAAGGTAACA
  ACACCATATACAGAGATTAGTGAAAATGTTTCAGATATTAAACAAATATGGGAAGGGGCA
  ATTGCTCAATGTTTAACTACTCAGGATTAA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  960
  1020
  1080
  1140
  1200
  1260
  1320
  1380
  1440
  1500
  1560
  1620
  1680
  1740
  1800
  1860
  1920
  1980
  2040
  2100
  2160
  2190
  

⊟Protein

⊟General

• locus tag: NWMN_0937 [new locus tag: NWMN_RS05250 ]
• symbol: PurL
• description: phosphoribosylformylglycinamidine synthase II
• length: 729
• theoretical pI: 4.50802
• theoretical MW: 79535.2
• GRAVY: -0.253086

⊟Function

• reaction:
  EC 6.3.5.3?  ExPASy
  Phosphoribosylformylglycinamidine synthase ATP + N²-formyl-N¹-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H₂O = ADP + phosphate + 2-(formamido)-N¹-(5-phospho-D-ribosyl)acetamidine + L-glutamate
• TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase II (TIGR01736; EC 6.3.5.3; HMM-score: 1004.7)
  and 8 more
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase (TIGR01857; EC 6.3.5.3; HMM-score: 166.4)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase (TIGR01735; EC 6.3.5.3; HMM-score: 139)
  Metabolism Energy metabolism Methanogenesis putative methanogenesis marker protein 2 (TIGR03267; HMM-score: 64.6)
  hydrogenase expression/formation protein HypE (TIGR02124; HMM-score: 41.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Thiamine thiamine-phosphate kinase (TIGR01379; EC 2.7.4.16; HMM-score: 37.9)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine cyclo-ligase (TIGR00878; EC 6.3.3.1; HMM-score: 37.1)
  Unknown function Enzymes of unknown specificity AIR synthase-related protein, sll0787 family (TIGR04049; HMM-score: 35.9)
  herpesvirus tegument protein/v-FGAM-synthase (TIGR01739; HMM-score: 12.6)
• TheSEED: data available for COL, N315, NCTC8325, USA300_FPR3757
• PFAM:
  no clan defined AIRS; AIR synthase related protein, N-terminal domain (PF00586; HMM-score: 157.8)
  AIRS_C; AIR synthase related protein, C-terminal domain (PF02769; HMM-score: 131.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: -1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.011831
  • TAT(Tat/SPI): 0.001013
  • LIPO(Sec/SPII): 0.00266
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 151221149 NCBI
• RefSeq: YP_001331971 NCBI
• UniProt: A6QFS7 UniProt

⊟Protein sequence

• MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTETGIFSVMWSEHCSYKHSKPFLKQFPTSGDHVLMGPGEGAGVVDIGDNQAVVFKVESHNHPSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGGYGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKTGRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGAAGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKFLDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNTSKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTNKAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIYQQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDFEPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGLLSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNIDNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: purQ > purL > purF > purM > purN > purH > purD

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications