PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS05495 [old locus tag: SACOL1077 ]
• pan locus tag^?: SAUPAN003281000
• symbol: SACOL_RS05495
• pan gene symbol^?: purQ
• synonym:
• product: phosphoribosylformylglycinamidine synthase subunit PurQ

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS05495 [old locus tag: SACOL1077 ]
• symbol: SACOL_RS05495
• product: phosphoribosylformylglycinamidine synthase subunit PurQ
• replicon: chromosome
• strand: +
• coordinates: 1085488..1086159
• length: 672
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (1085488..1086159) NCBI
• BioCyc: SACOL_RS05495 BioCyc
• MicrobesOnline: see SACOL1077

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

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  ATGAAATTTGCGGTTCTTGTTTTTCCAGGTTCGAATTGTGATAGAGACATGTTTAATGCT
  GCTATTAAAAGTGGTGTTGAAGCGGAATATGTAGATTATAGAGAAACATCACTAAGTGGA
  TTTGATGGCGTACTTATTCCTGGTGGATTTTCATTCGGGGATTACTTAAGATCTGGGGCA
  ATGGCTAGTGTAGCGCCGATTATTTCGGAAGTTAAACGTCTTGCAGCTGAAGGTAAGCCA
  GTATTAGGTGTTTGTAATGGGTTTCAAATTTTAACTGAAATAGGCTTATTACCTGGTGCA
  TTATTGCATAACGATTCACATTTATTTATTAGTAGAAATGAAGAGTTAGAAATAGTGAAT
  AATCAAACGGCATTTACAAATCTTTATGAACAAGGTGAAAAAGTTATATATCCTGTAGCT
  CACGGTGAAGGTCATTATTATTGTACTGATGAAATATATCAACAATTAAAAGCTAACAAT
  CAAATTATTCTGAAATATGTGAATAATCCGAACGGTTCATATGATGATATTGCAGGAATT
  GTTAACGAAAAAGGCAATGTATGTGGCATGATGCCACATCCTGAAAGAGCTTTAGAAACG
  TTGTTAGGTACTGATAGTGGTGTGAAATTATTTGAAGCGATGGTAAAAAGTTGGAGGGAA
  CAACATGTCTAA
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⊟Protein

⊟General

• locus tag: SACOL_RS05495 [old locus tag: SACOL1077 ]
• symbol: SACOL_RS05495
• description: phosphoribosylformylglycinamidine synthase subunit PurQ
• length: 223
• theoretical pI: 4.69597
• theoretical MW: 24526.7
• GRAVY: -0.108969

⊟Function

• reaction:
  EC 3.5.1.2?  ExPASy
  Glutaminase L-glutamine + H₂O = L-glutamate + NH₃
  EC 6.3.5.3?  ExPASy
  Phosphoribosylformylglycinamidine synthase ATP + N²-formyl-N¹-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H₂O = ADP + phosphate + 2-(formamido)-N¹-(5-phospho-D-ribosyl)acetamidine + L-glutamate
• TIGRFAM:
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase I (TIGR01737; EC 6.3.5.3; HMM-score: 325.8)
  and 10 more
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase (TIGR01857; EC 6.3.5.3; HMM-score: 83.2)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis phosphoribosylformylglycinamidine synthase (TIGR01735; EC 6.3.5.3; HMM-score: 76.2)
  Genetic information processing Protein fate Degradation of proteins, peptides, and glycopeptides intracellular protease, PfpI family (TIGR01382; HMM-score: 25.8)
  Metabolism Amino acid biosynthesis Histidine family imidazole glycerol phosphate synthase, glutamine amidotransferase subunit (TIGR01855; EC 2.4.2.-; HMM-score: 25.4)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pyridoxine pyridoxal 5'-phosphate synthase, glutaminase subunit Pdx2 (TIGR03800; EC 2.6.-.-; HMM-score: 21.8)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis CTP synthase (TIGR00337; EC 6.3.4.2; HMM-score: 17.2)
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobyric acid synthase CobQ (TIGR00313; EC 6.3.5.10; HMM-score: 17.1)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis GMP synthase (glutamine-hydrolyzing), N-terminal domain (TIGR00888; EC 6.3.5.2; HMM-score: 16.2)
  Metabolism Purines, pyrimidines, nucleosides, and nucleotides Pyrimidine ribonucleotide biosynthesis carbamoyl-phosphate synthase, small subunit (TIGR01368; EC 6.3.5.5; HMM-score: 13.2)
  aminodeoxychorismate synthase (TIGR01823; EC 2.6.1.85; HMM-score: 10.2)
• TheSEED: see SACOL1077
• PFAM:
  Glutaminase_I (CL0014) GATase_5; CobB/CobQ-like glutamine amidotransferase domain (PF13507; HMM-score: 155.3)
  and 5 more
  GATase; Glutamine amidotransferase class-I (PF00117; HMM-score: 32.7)
  GATase_3; CobB/CobQ-like glutamine amidotransferase domain (PF07685; HMM-score: 30)
  DJ-1_PfpI; DJ-1/PfpI family (PF01965; HMM-score: 29)
  Peptidase_C26; Peptidase C26 (PF07722; HMM-score: 16.4)
  SNO; SNO glutamine amidotransferase family (PF01174; HMM-score: 13.9)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.045141
  • TAT(Tat/SPI): 0.000764
  • LIPO(Sec/SPII): 0.006095
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446589453 NCBI
• RefSeq: WP_000666799 NCBI
• UniProt: see SACOL1077

⊟Protein sequence

• MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGVLIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALLHNDSHLFISRNEELEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANNQIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSWREQHV

⊟Experimental data

• experimentally validated: see SACOL1077
• protein localization: see SACOL1077
• quantitative data / protein copy number per cell: see SACOL1077
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• data available for N315

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL1077

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications