PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159JSNZLGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS04270 [old locus tag: SACOL0830 ]
• pan locus tag^?: SAUPAN002689000
• symbol: SACOL_RS04270
• pan gene symbol^?: —
• synonym:
• product: RNase adaptor protein RapZ

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS04270 [old locus tag: SACOL0830 ]
• symbol: SACOL_RS04270
• product: RNase adaptor protein RapZ
• replicon: chromosome
• strand: +
• coordinates: 856421..857332
• length: 912
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (856421..857332) NCBI
• BioCyc: SACOL_RS04270 BioCyc
• MicrobesOnline: see SACOL0830

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
  61
  121
  181
  241
  301
  361
  421
  481
  541
  601
  661
  721
  781
  841
  901
  
  ATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCT
  GGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGAT
  AATCTACCACCAGTGTTATTGCCTAAATTTGTAGAGTTGATGGAACAAGGAAATCCATCC
  TTAAGAAAAGTGGCAATTGCAATTGATTTAAGAGGTAAGGAACTATTTAATTCATTAGTT
  GCAGTAGTGGATAAAGTCAAAAGTGAAAGTGACGTCATCATTGATGTTATGTTTTTAGAA
  GCAAGTACTGAAAAATTAATTTCAAGATATAAGGAAACGCGTCGTGCACATCCTTTGATG
  GAACAAGGTAAAAGATCGTTAATCAATGCAATTAATGATGAGCGAGAGCATTTGTCTCAA
  ATTAGAAGTATAGCTAATTTTGTTATAGATACTACAAAGTTATCACCTAAAGAATTAAAA
  GAACGCATTCGTCGATACTATGAAGATGAAGAGTTTGAAACTTTTACAATTAATGTCACA
  AGTTTCGGTTTTAAACATGGGATTCAGATGGATGCAGATTTAGTATTTGATGTACGATTT
  TTACCAAATCCATATTATGTAGTAGATTTAAGACCTTTAACAGGATTAGATAAAGACGTT
  TATAATTATGTTATGAAATGGAAAGAGACGGAGATTTTCTTTGAAAAATTAACTGATTTG
  TTAGATTTTATGATACCCGGGTATAAAAAAGAAGGGAAATCTCAATTAGTAATTGCCATC
  GGTTGTACGGGTGGACAACATCGATCTGTAGCATTAGCAGAACGACTAGGTAATTATCTA
  AATGAAGTATTTGAATATAATGTTTATGTGCATCATAGGGACGCACATATTGAAAGTGGC
  GAGAAAAAATGA
  60
  120
  180
  240
  300
  360
  420
  480
  540
  600
  660
  720
  780
  840
  900
  912
  

⊟Protein

⊟General

• locus tag: SACOL_RS04270 [old locus tag: SACOL0830 ]
• symbol: SACOL_RS04270
• description: RNase adaptor protein RapZ
• length: 303
• theoretical pI: 5.71204
• theoretical MW: 34811.8
• GRAVY: -0.30066

⊟Function

• TIGRFAM:
  Metabolism Transport and binding proteins Anions phosphonate ABC transporter, ATP-binding protein (TIGR02315; EC 3.6.3.28; HMM-score: 15.2)
  Metabolism Central intermediary metabolism Sulfur metabolism adenylyl-sulfate kinase (TIGR00455; EC 2.7.1.25; HMM-score: 14.7)
• TheSEED: see SACOL0830
• PFAM:
  P-loop_NTPase (CL0023) RapZ-like_N; RapZ-like N-terminal domain (PF03668; HMM-score: 167.3)
  Phosphatase (CL0031) PapZ_C; RapZ C-terminal domain (PF22740; HMM-score: 166.7)
  and 13 more
  P-loop_NTPase (CL0023) SKI; Shikimate kinase (PF01202; HMM-score: 18.9)
  AAA_22; AAA domain (PF13401; HMM-score: 18)
  AAA_18; AAA domain (PF13238; HMM-score: 16.9)
  Phage_TACs (CL0567) Phage_TAC_3; Phage tail assembly chaperone proteins, TAC (PF06896; HMM-score: 15.9)
  P-loop_NTPase (CL0023) AAA_33; AAA domain (PF13671; HMM-score: 15.6)
  DnaB_C; DnaB-like helicase C terminal domain (PF03796; HMM-score: 15.5)
  AAA_16; AAA ATPase domain (PF13191; HMM-score: 14.7)
  AAA_21; AAA domain, putative AbiEii toxin, Type IV TA system (PF13304; HMM-score: 14.6)
  PGM_I-III (CL0754) PGM_PMM_II; Phosphoglucomutase/phosphomannomutase, alpha/beta/alpha domain II (PF02879; HMM-score: 14)
  P-loop_NTPase (CL0023) ABC_tran; ABC transporter (PF00005; HMM-score: 13.4)
  Rad17; Rad17 P-loop domain (PF03215; HMM-score: 13.3)
  TPR (CL0020) DUF1186; Protein of unknown function (DUF1186) (PF06685; HMM-score: 13.2)
  P-loop_NTPase (CL0023) RsgA_GTPase; RsgA GTPase (PF03193; HMM-score: 12)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 7.5
  • Cytoplasmic Membrane Score: 1.15
  • Cellwall Score: 0.62
  • Extracellular Score: 0.73
  • Internal Helices: 0
• DeepLocPro: Cytoplasmic
  • Cytoplasmic Score: 0.9184
  • Cytoplasmic Membrane Score: 0.0111
  • Cell wall & surface Score: 0.0001
  • Extracellular Score: 0.0705
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.017699
  • TAT(Tat/SPI): 0.006084
  • LIPO(Sec/SPII): 0.015606
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446291867 NCBI
• RefSeq: WP_000369722 NCBI
• UniProt: see SACOL0830

⊟Protein sequence

• MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPPVLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEASTEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELKERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDKDVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERLGNYLNEVFEYNVYVHHRDAHIESGEKK

⊟Experimental data

• experimentally validated: see SACOL0830
• protein localization: see SACOL0830
• quantitative data / protein copy number per cell: see SACOL0830
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL0830

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications