PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS01705 [old locus tag: SACOL0339 ]
• pan locus tag^?: SAUPAN001442000
• symbol: SACOL_RS01705
• pan gene symbol^?: ssb1
• synonym:
• product: Single-stranded DNA-binding protein 2

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS01705 [old locus tag: SACOL0339 ]
• symbol: SACOL_RS01705
• product: Single-stranded DNA-binding protein 2
• replicon: chromosome
• strand: +
• coordinates: 363651..364070
• length: 420
• essential: unknown

⊟Accession numbers

• Location: NC_002951 (363651..364070) NCBI
• BioCyc: SACOL_RS01705 BioCyc
• MicrobesOnline: see SACOL0339

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGTTAAACAGAACAGTATTAGTAGGGCGATTAACAAAAGACCCAGAATTAAGAAGCACG
  CCAAATGGCGTAAATGTAGGGACATTCACATTAGCAGTAAACAGAACATTTACAAATGCT
  CAAGGCGAGCGTGAAGCAGACTTTATAAACGTAGTAGTATTCAAAAAACAAGCTGAAAAC
  GTTAAAAACTACCTTTCTAAAGGATCACTGGCAGGTGTAGACGGGCGATTACAAACACGC
  AGTTACGATAACAAAGAAGGGCGACGTGTATTTGTGACAGAAGTAGTAGCGGACAGCGTT
  CAATTCTTAGAACCGAAGAATAACAACAAACAGAATAACCAACAACACAACGGACAAACT
  CAAACTGGTAATAATCCGTTCGACAATACCGAAGAAGACTTTTCTGACTTACCGTTCTGA
  60
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  420
  

⊟Protein

⊟General

• locus tag: SACOL_RS01705 [old locus tag: SACOL0339 ]
• symbol: SACOL_RS01705
• description: Single-stranded DNA-binding protein 2
• length: 139
• theoretical pI: 7.53655
• theoretical MW: 15608.1
• GRAVY: -0.815108

⊟Function

• TIGRFAM:
  Genetic information processing DNA metabolism DNA replication, recombination, and repair single-stranded DNA-binding protein (TIGR00621; HMM-score: 140.6)
  and 1 more
  Genetic information processing DNA metabolism DNA replication, recombination, and repair primosomal replication protein PriB (TIGR04418; HMM-score: 34.2)
• TheSEED: see SACOL0339
• PFAM:
  OB (CL0021) SSB; Single-strand binding protein family (PF00436; HMM-score: 123)
  and 1 more
  tRNA_anti-codon; OB-fold nucleic acid binding domain (PF01336; HMM-score: 20.3)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.67
  • Cytoplasmic Membrane Score: 0.01
  • Cellwall Score: 0.15
  • Extracellular Score: 0.17
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.010636
  • TAT(Tat/SPI): 0.001159
  • LIPO(Sec/SPII): 0.001559
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446857521 NCBI
• RefSeq: WP_000934777 NCBI
• UniProt: see SACOL0339

⊟Protein sequence

• MLNRTVLVGRLTKDPELRSTPNGVNVGTFTLAVNRTFTNAQGEREADFINVVVFKKQAENVKNYLSKGSLAGVDGRLQTRSYDNKEGRRVFVTEVVADSVQFLEPKNNNKQNNQQHNGQTQTGNNPFDNTEEDFSDLPF

⊟Experimental data

• experimentally validated: see SACOL0339
• protein localization: see SACOL0339
• quantitative data / protein copy number per cell:
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator:

⊟Transcription pattern

• S.aureus Expression Data Browser: no data available

⊟Protein synthesis (provided by Aureolib)

• Aureolib: no data available

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications