PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 02-MAR-2017

⊟Summary

• organism: Staphylococcus aureus COL
• locus tag: SACOL_RS00765 [old locus tag: SACOL0151 ]
• pan locus tag^?: SAUPAN000994000
• symbol: SACOL_RS00765
• pan gene symbol^?: capP
• synonym:
• product: UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SACOL_RS00765 [old locus tag: SACOL0151 ]
• symbol: SACOL_RS00765
• product: UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)
• replicon: chromosome
• strand: +
• coordinates: 168828..169973
• length: 1146
• essential: unknown other strains

⊟Accession numbers

• Location: NC_002951 (168828..169973) NCBI
• BioCyc: SACOL_RS00765 BioCyc
• MicrobesOnline: see SACOL0151

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGAAAAAAATTATGGTTATTTTCGGTACGAGACCCGAAGCAATAAAAATGGCACCATTA
  GTAAAAGAAATTGATCATAATGGGAACTTTGAAGCGAACATTGTGATTACAGCACAACAT
  AGAGATATGTTAGATAGTGTGTTAAGTATATTTGATATTCAAGCTGATCATGATTTAAAT
  ATTATGCAAGATCAACAAACATTAGCAGGCCTTACGGCGAATGCACTTGCTAAACTTGAT
  AGCATCATTAATGAGGAACAACCGGATATGATTTTAGTACATGGTGATACTACAACGACT
  TTTGTAGGAAGTTTGGCAGCATTTTATCATCAAATTCCGGTCGGACATGTAGAAGCTGGA
  CTTCGAACACATCAGAAATACTCACCATTTCCTGAAGAGTTAAATCGAGTCATGGTAAGT
  AATATTGCTGAATTGAATTTTGCGCCAACAGTAATTGCAGCTAAAAATTTACTTTTTGAA
  AACAAAGACAAAGAGCGTATCTTTATTACTGGAAATACAGTTATTGACGCATTGTCAACA
  ACAGTTCAAAATGATTTTGTTTCAACGATTATTAATAAACATAAAGGCAAGAAAGTTGTT
  TTACTAACAGCGCATCGTCGTGAAAATATTGGGGAACCGATGCATCAGATTTTTAAAGCA
  GTAAGAGATTTGGCAGATGAATATAAAGATGTTGTCTTCATTTATCCAATGCATCGTAAT
  CCAAAGGTAAGAGCGATTGCCGAAAAATATTTATCTGGGAGAAATCGGATTGAATTAATT
  GAGCCATTAGATGCGATTGAGTTCCATAATTTTACAAATCAATCGTACCTCGTGCTGACA
  GATTCTGGTGGTATTCAAGAGGAGGCTCCTACATTTGGAAAACCTGTGTTGGTATTAAGG
  AATCATACAGAGCGTCCCGAAGGCGTTGAGGCGGGAACATCGAGAGTAATTGGCACAGAT
  TATGACAATATTGTTCGAAATGTGAAACAATTGATTGAGGATGATGAAGCGTATCAACGT
  ATGAGTCAAGCGAATAATCCATATGGTGATGGACAAGCATCACGACGTATTTGTGAAGCA
  ATAGAATATTATTTTGGATTGCGCACAGACAAGCCGGATGAATTCGTACCTTTACGTCAC
  AAATAA
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⊟Protein

⊟General

• locus tag: SACOL_RS00765 [old locus tag: SACOL0151 ]
• symbol: SACOL_RS00765
• description: UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing)
• length: 381
• theoretical pI: 6.02477
• theoretical MW: 43091.7
• GRAVY: -0.330446

⊟Function

• reaction:
  EC 5.1.3.14?  ExPASy
  UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing) UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-mannosamine
• TIGRFAM:
  Cell structure Cell envelope Biosynthesis and degradation of surface polysaccharides and lipopolysaccharides UDP-N-acetylglucosamine 2-epimerase (TIGR00236; EC 5.1.3.14; HMM-score: 515.7)
  and 2 more
  UDP-N-acetyl-D-glucosamine 2-epimerase, UDP-hydrolysing (TIGR03568; EC 3.1.4.-,5.1.3.-; HMM-score: 119.3)
  conserved hypothetical protein (TIGR03492; HMM-score: 24.3)
• TheSEED: see SACOL0151
• PFAM:
  GT-B (CL0113) Epimerase_2; UDP-N-acetylglucosamine 2-epimerase (PF02350; HMM-score: 402)
  and 4 more
  Glyphos_transf; CDP-Glycerol:Poly(glycerophosphate) glycerophosphotransferase (PF04464; HMM-score: 19.1)
  Glyco_trans_4_2; Glycosyl transferase 4-like (PF13477; HMM-score: 18.3)
  LpxB; Lipid-A-disaccharide synthetase (PF02684; HMM-score: 13.3)
  Glycos_transf_1; Glycosyl transferases group 1 (PF00534; HMM-score: 13.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors:
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP:
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.00427
  • TAT(Tat/SPI): 0.000064
  • LIPO(Sec/SPII): 0.000408
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 446646108 NCBI
• RefSeq: WP_000723454 NCBI
• UniProt: see SACOL0151

⊟Protein sequence

• MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDMLDSVLSIFDIQADHDLNIMQDQQTLAGLTANALAKLDSIINEEQPDMILVHGDTTTTFVGSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFENKDKERIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVVLLTAHRRENIGEPMHQIFKAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSYLVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIEDDEAYQRMSQANNPYGDGQASRRICEAIEYYFGLRTDKPDEFVPLRHK

⊟Experimental data

• experimentally validated: see SACOL0151
• protein localization: see SACOL0151
• quantitative data / protein copy number per cell: see SACOL0151
• interaction partners:

⊟Expression & Regulation

⊟Operon

⊟Regulation

• regulator: CodY, SigB* see SACOL0151

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: see SACOL0151

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications