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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2659 [new locus tag: SACOL_RS13930 ]
  • pan locus tag?: SAUPAN006367000
  • symbol: aur
  • pan gene symbol?: aur
  • synonym:
  • product: zinc metalloproteinase aureolysin

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2659 [new locus tag: SACOL_RS13930 ]
  • symbol: aur
  • product: zinc metalloproteinase aureolysin
  • replicon: chromosome
  • strand: -
  • coordinates: 2720850..2722379
  • length: 1530
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    GTGAGGAAATTTTCAAGATATGCATTTACAAGTATGGCAGCATTAACCTTGTTGAGCACT
    TTATCACCAGCAGCATTAGCGATTGATTCAAAAAATAAACCAGCTAATTCTGATATTAAA
    TTTGAGGTGACTCAAAAGAGTGATGCGGTCAAAGCATTAAAAGAATTGCCTAAATCCGAA
    AATGTAAAAAATATTTATCAAGATTACGCTGTTACTGATGTAAAAACTGATAAAAAAGGA
    TTTACGCATTATACATTGCAACCGAGTGTTGATGGTGTTCATGCACCTGACAAAGAAGTG
    AAAGTACACGCAGACAAATCAGGAAAAGTCGTTTTAATCAATGGGGATACTGATGCGAAG
    AAAGTAAAGCCAACGAATAAAGTGACATTAAGTAAAGATGACGCAGCCGACAAAGCATTT
    AAAGCAGTTAAGATTGATAAGAATAAAGCGAAAAATCTTAAAGATAAAGTCATTAAAGAA
    AACAAAGTTGAAATCGATGGTGACAGTAATAAATACGTTTATAATGTTGAGTTAATTACA
    GTGACACCAGAAATTTCACATTGGAAAGTTAAAATTGATGCTCAAACTGGCGAAATTTTA
    GAAAAAATGAACTTAGTTAAAGAAGCTGCAGAAACTGGTAAAGGAAAAGGTGTACTTGGC
    GATACAAAAGATATCAATATCAATAGTATTGACGGTGGATTTAGCCTAGAAGATTTAACG
    CATCAAGGTAAATTATCAGCATTTAGCTTTAATGATCAAACAGGTCAAGCAACATTGATT
    ACTAATGAAGATGAAAACTTCGTAAAAGATGAGCAACGTGCTGGCGTAGATGCAAATTAT
    TACGCTAAACAAACATATGATTATTACAAAGACACATTTGGTCGTGAATCATATGACAAC
    CAAGGTAGTCCAATTGTTTCATTAACGCATGTTAATAACTACGGTGGTCAAGATAACAGA
    AATAATGCCGCATGGATCGGTGACAAAATGATCTATGGTGATGGTGATGGTCGCACATTC
    ACAAGTTTATCGGGTGCAAATGACGTAGTAGCACACGAATTAACACACGGTGTGACACAA
    GAGACAGCGAACTTAGAATATAAGGACCAGTCAGGCGCTCTAAATGAAAGCTTTTCAGAT
    GTTTTTGGATACTTTGTAGATGACGAGGATTTCTTAATGGGTGAAGATGTCTACACACCT
    GGAAAAGAGGGAGACGCTTTACGCAGCATGTCAAACCCAGAACAATTTGGTCAACCAGCT
    CATATGAAAGACTATGTATTCACTGAAAAAGATAATGGTGGCGTACATACGAATTCTGGA
    ATTCCAAATAAAGCAGCTTATAACGTGATTCAAGCAATAGGGAAATCTAAATCAGAACAA
    ATTTACTACCGAGCATTAACGGAATACTTAACAAGTAATTCAAACTTCAAAGATTGTAAA
    GATGCATTATACCAAGCGGCTAAAGATTTATATGACGAGCAAACAGCTGAACAGGTGTAT
    GAAGCATGGAATGAAGTAGGCGTGGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1530

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2659 [new locus tag: SACOL_RS13930 ]
  • symbol: Aur
  • description: zinc metalloproteinase aureolysin
  • length: 509
  • theoretical pI: 4.88318
  • theoretical MW: 56361
  • GRAVY: -0.700589

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.24.29?  ExPASy
    Aureolysin Cleavage of insulin B chain with specificity similar to that of thermolysin, preferring hydrophobic P1' residues. Activates the glutamyl endopeptidase (EC 3.4.21.19) of Staphylococcus aureus
  • TIGRFAM:
  • TheSEED  :
    • aureolysin
    • Zinc metalloproteinase precursor (EC 3.4.24.29)
    Phages, Prophages, Transposable elements, Plasmids Pathogenicity islands Listeria Pathogenicity Island LIPI-1 extended  Zinc metalloproteinase precursor (EC 3.4.24.29)
  • PFAM:
    Peptidase_MA (CL0126) Peptidase_M4_C; Thermolysin metallopeptidase, alpha-helical domain (PF02868; HMM-score: 176.1)
    Peptidase_M4; Thermolysin metallopeptidase, catalytic domain (PF01447; HMM-score: 142.8)
    and 2 more
    PepSY (CL0320) PepSY; Peptidase propeptide and YPEB domain (PF03413; HMM-score: 50.1)
    Cystatin (CL0121) FTP; Fungalysin/Thermolysin Propeptide Motif (PF07504; HMM-score: 47.7)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • LocateP: Secretory(released) (with CS)
    • Prediction by SwissProt Classification: Extracellular
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: -0.17
    • Signal peptide possibility: 1
    • N-terminally Anchored Score: -1
    • Predicted Cleavage Site: PAALAIDS
  • SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.985978
    • TAT(Tat/SPI): 0.011314
    • LIPO(Sec/SPII): 0.002036
    • Cleavage Site: CS pos: 27-28. ALA-ID. Pr: 0.9577
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRKFSRYAFTSMAALTLLSTLSPAALAIDSKNKPANSDIKFEVTQKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKVHADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKENKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGVLGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGVDANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGDGDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFLMGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVIQAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGVE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2] [3] [4]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: CodY (repression) regulon
    CodY(TF)important in Amino acid metabolism; RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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