From AureoWiki
Jump to navigation Jump to search

FunGene: 08-OCT-2024

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus JSNZ
  • locus tag: JSNZ_002627
  • pan locus tag?: SAUPAN006367000
  • symbol: aur
  • pan gene symbol?: aur
  • synonym:
  • product: zinc metalloproteinase aureolysin

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: JSNZ_002627
  • symbol: aur
  • product: zinc metalloproteinase aureolysin
  • replicon: chromosome
  • strand: -
  • coordinates: 2632406..2633935
  • length: 1530
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

  • Gene ID:
  • RefSeq:
  • BioCyc:
  • MicrobesOnline:

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    GTGAGGAAATTTTCAAGATATGCATTTACAAGTATGGCAGCAGTAACATTGTTGAGCACT
    TTATCACCAGCAGCATTAGCGATTGATTCAAAAAATAAACCAGCTAATTCTGATATTAAA
    TTTGAGGTGACTCAAAAGAGTGATGCGGTCAAAGCATTAAAAGAATTGCCTAAATCCGAA
    AATGTAAAAAATATTTATCAAGATTACGCTGTTACTGATGTAAAAACTGATAAAAAAGGA
    TTTACGCATTATACATTGCAACCGAGTGTTGATGGTGTTCATGCACCTGACAAAGAAGTG
    AAAGTACACGCAGACAAATCAGGAAAAGTCGTTTTAATCAATGGGGATACTGATGCGAAG
    AAAGTAAAGCCAACGAATAAAGTGACATTAAGTAAAGATGACGCAGCCGACAAAGCATTT
    AAAGCAGTTAAGATTGATAAGAATAAAGCGAAAAATCTTAAAGATAAAGTCATTAAAGAA
    AACAAAGTTGAAATCGATGGTGACAGTAATAAATACGTTTATAATGTTGAGTTAATTACA
    GTGACACCAGAAATTTCACATTGGAAAGTTAAAATTGATGCTCAAACTGGCGAAATTTTA
    GAAAAAATGAACTTAGTTAAAGAAGCTGCAGAAACTGGTAAAGGAAAAGGTGTACTTGGC
    GATACAAAAGATATCAATATCAATAGTATTGACGGTGGATTTAGCCTAGAAGATTTAACG
    CATCAAGGTAAATTATCAGCATTTAGCTTTAATGATCAAACAGGTCAAGCAACATTGATT
    ACTAATGAAGATGAAAACTTCGTAAAAGATGAGCAACGTGCTGGCGTAGATGCAAATTAT
    TACGCTAAACAAACATATGATTATTACAAAGACACATTTGGTCGTGAATCATATGACAAC
    CAAGGTAGTCCAATTGTTTCATTAACGCATGTTAATAACTACGGTGGTCAAGATAACAGA
    AATAATGCCGCATGGATTGGTGACAAAATGATCTATGGTGATGGTGATGGTCGCACATTC
    ACAAGTTTATCGGGTGCAAATGACGTAGTAGCACACGAATTAACACACGGTGTGACACAA
    GAGACTGCAAACTTAGAATATAAGGACCAGTCAGGCGCTCTAAATGAAAGCTTTTCAGAT
    GTTTTTGGATACTTTGTAGATGACGAGGATTTCTTAATGGGTGAAGATGTCTACACACCT
    GGAAAAGAGGGAGACGCTTTACGCAGCATGTCAAACCCAGAACAATTTGGTCAACCAGCT
    CATATGAAAGACTATGTATTCACTGAAAAAGATAATGGTGGCGTACATACGAATTCTGGA
    ATTCCAAATAAAGCAGCTTATAACGTGATTCAAGCAATAGGGAAATCTAAATCAGAACAA
    ATTTACTACCGAGCATTAACGGAATACTTAACAAGTAATTCAAACTTCAAAGATTGTAAA
    GATGCATTATACCAAGCGGCTAAAGATTTATATGACGAGCAAACAGCTGAACAGCTGTAT
    GAAGCATGGAATGAAGTAGGCGTGGAGTAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1440
    1500
    1530

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: JSNZ_002627
  • symbol: Aur
  • description: zinc metalloproteinase aureolysin
  • length: 509
  • theoretical pI: 4.88318
  • theoretical MW: 56361
  • GRAVY: -0.700589

⊟Function[edit | edit source]

  • reaction:
    EC 3.4.24.29?  ExPASy
    Aureolysin Cleavage of insulin B chain with specificity similar to that of thermolysin, preferring hydrophobic P1' residues. Activates the glutamyl endopeptidase (EC 3.4.21.19) of Staphylococcus aureus
  • TIGRFAM:
  • TheSEED: data available for COL, N315, NCTC8325, Newman, USA300_FPR3757
  • PFAM:
    Peptidase_MA (CL0126) Peptidase_M4_C; Thermolysin metallopeptidase, alpha-helical domain (PF02868; HMM-score: 174.2)
    Peptidase_M4; Thermolysin metallopeptidase, catalytic domain (PF01447; HMM-score: 145.2)
    and 4 more
    PepSY (CL0320) FTP; Fungalysin/Thermolysin Propeptide Motif (PF07504; HMM-score: 48.9)
    PepSY; Peptidase propeptide and YPEB domain (PF03413; HMM-score: 33)
    no clan defined BCAS3; BCAS3 microtubule associated cell migration factor, C-terminal (PF12490; HMM-score: 13.1)
    Flagellin_D3; Flagellin D3 domain (PF08884; HMM-score: 8.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.02
    • Extracellular Score: 9.98
    • Internal Helices: 0
  • DeepLocPro: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.0002
    • Cell wall & surface Score: 0.009
    • Extracellular Score: 0.9908
  • LocateP:
  • SignalP: Signal peptide SP(Sec/SPI) length 27 aa
    • SP(Sec/SPI): 0.983808
    • TAT(Tat/SPI): 0.013368
    • LIPO(Sec/SPII): 0.002057
    • Cleavage Site: CS pos: 27-28. ALA-ID. Pr: 0.9552
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

  • GI:
  • RefSeq:
  • UniProt:

⊟Protein sequence[edit | edit source]

  • MRKFSRYAFTSMAAVTLLSTLSPAALAIDSKNKPANSDIKFEVTQKSDAVKALKELPKSENVKNIYQDYAVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKVHADKSGKVVLINGDTDAKKVKPTNKVTLSKDDAADKAFKAVKIDKNKAKNLKDKVIKENKVEIDGDSNKYVYNVELITVTPEISHWKVKIDAQTGEILEKMNLVKEAAETGKGKGVLGDTKDININSIDGGFSLEDLTHQGKLSAFSFNDQTGQATLITNEDENFVKDEQRAGVDANYYAKQTYDYYKDTFGRESYDNQGSPIVSLTHVNNYGGQDNRNNAAWIGDKMIYGDGDGRTFTSLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFLMGEDVYTPGKEGDALRSMSNPEQFGQPAHMKDYVFTEKDNGGVHTNSGIPNKAAYNVIQAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQLYEAWNEVGVE

⊟Experimental data[edit | edit source]

  • experimentally validated: data available for COL, NCTC8325
  • protein localization: data available for COL
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulators: Rex* (repression) regulon, CodY (repression) regulon
    Rex*(TF)important in Energy metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]
    CodY(TF)important in Amino acid metabolism;  regulation predicted or transferred from N315 and NCTC 8325  [1]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ 1.0 1.1 Hannes Wolfgramm, Larissa Milena Busch, Jöran Tebben, Henry Mehlan, Lisa Hagenau, Thomas Sura, Tilly Hoffmüller, Elisa Bludau, Manuela Gesell Salazar, Alexander Reder, Stephan Michalik, Leif Steil, Kristin Surmann, Ulrike Mäder, Silva Holtfreter, Uwe Völker
    Integrated genomic and proteomic analysis of the mouse-adapted Staphylococcus aureus strain JSNZ.
    Curr Res Microb Sci: 2025, 9;100489
    [PubMed:41146725] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

Retrieved from "http://fungenwikiserver.biologie.uni-greifswald.de/aureowiki/index.php?title=JSNZ_002627&oldid=108268"