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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2298 [new locus tag: SACOL_RS12090 ]
  • pan locus tag?: SAUPAN005782000
  • symbol: SACOL2298
  • pan gene symbol?: —
  • synonym:
  • product: N-acetylmuramoyl-L-alanine amidase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2298 [new locus tag: SACOL_RS12090 ]
  • symbol: SACOL2298
  • product: N-acetylmuramoyl-L-alanine amidase
  • replicon: chromosome
  • strand: -
  • coordinates: 2359394..2360170
  • length: 777
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGAAGAAGAATTTCAAGTTACGCATTTCAACGCTACTATTGATAGTTATTTTAGTTGTT
    TTTGCTGTATTACTCATCGTGAATGAAACTAAATTGTTTAAAAATGATGTGAATTACTCT
    TTTGATGAGGCTGTTTCAATGCAACAAGGGAAAGGTATTGTACAGACAAAAGAAGAGGAT
    GGTAAATTTGTTGAAGCAAATAATAATGAAATTGCTAAAGCAATGACTATTTCACATAAA
    GACAATGATATGAAGTATATGGATATCACAGAAAAAGTGCCAATGTCGGAATCTGAAGTT
    AACCAATTGCTAAAAGGTAAGGGGATTTTAGAAAATCGAGGGAAAGTTTTTCTAGAAGCT
    CAAGAAAAATATGAGGTTAATGTCATTTATCTTGTTAGCCATGCATTAGTAGAAACAGGT
    AACGGCAAATCAGAATTAGCAAAAGGCATTAAAGATGGGAAAAAACGCTATTACAACTTT
    TTTGGTATAGGAGCATTCGATAGTAGTGCTGTTCGTAGTGGGAAAAGTTATGCTGAAAAG
    GAACAATGGACATCACCAGATAAGGCGATTATTGGTGGTGCAAAGTTCATTCGTAATGAA
    TATTTTGAAAACAATCAACTGAATTTATATCAAATGCGATGGAATCCAGAAAATCCTGCG
    CAACATCAATATGCGAGTGACATTCGCTGGGCAGATAAAATTGCCAAATTAATGGATAAA
    TCCTATAAGCAGTTTGGTATAAAGAAAGATGATATTAGACAAACATATTATAAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    777

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2298 [new locus tag: SACOL_RS12090 ]
  • symbol: SACOL2298
  • description: N-acetylmuramoyl-L-alanine amidase
  • length: 258
  • theoretical pI: 9.60613
  • theoretical MW: 29671.8
  • GRAVY: -0.555039

⊟Function[edit | edit source]

  • TIGRFAM:
  • TheSEED  :
    • Bifunctional autolysin Atl
    • Endo-beta-N-acetylglucosaminidase (EC 3.2.1.96)
    • N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Murein Hydrolases  N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
    and 3 more
    Cell Wall and Capsule Cell Wall and Capsule - no subcategory Recycling of Peptidoglycan Amino Acids  N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28)
    Regulation and Cell signaling Quorum sensing and biofilm formation Biofilm formation in Staphylococcus  Bifunctional autolysin Atl
    Virulence, Disease and Defense Adhesion Adhesins in Staphylococcus  Bifunctional autolysin Atl
  • PFAM:
    Lysozyme (CL0037) Glucosaminidase; Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase (PF01832; HMM-score: 65.9)
    and 1 more
    no clan defined Dcc1; Sister chromatid cohesion protein Dcc1 (PF09724; HMM-score: 16.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Extracellular
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 0.09
    • Cellwall Score: 0.18
    • Extracellular Score: 9.73
    • Internal Helix: 1
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0.0021
    • Cytoplasmic Membrane Score: 0.5861
    • Cell wall & surface Score: 0.0048
    • Extracellular Score: 0.407
  • LocateP: N-terminally anchored (No CS)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPI)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0
    • N-terminally Anchored Score: 7
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.419066
    • TAT(Tat/SPI): 0.000696
    • LIPO(Sec/SPII): 0.031158
  • predicted transmembrane helices (TMHMM): 1

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEAVSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQLLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNFFGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPENPAQHQYASDIRWADKIAKLMDKSYKQFGIKKDDIRQTYYK

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Signal peptide containing [1] [2]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  2. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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