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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL2208 [new locus tag: SACOL_RS11625 ]
  • pan locus tag?: SAUPAN005669000
  • symbol: truA
  • pan gene symbol?: truA
  • synonym:
  • product: tRNA pseudouridine synthase A

⊟Additional information (user-provided)[edit | edit source]

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL2208 [new locus tag: SACOL_RS11625 ]
  • symbol: truA
  • product: tRNA pseudouridine synthase A
  • replicon: chromosome
  • strand: -
  • coordinates: 2289763..2290566
  • length: 804
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGCGTATATTAGTAGAAATTGCGTATCAAGGAAATAATTTTCTAGGTTTTCAAATTCAA
    CAAAATGGTCGAACGGTACAGCAACAATTTGAAAAGCTATTACAACGCATGCATAAAAGA
    CATGTGAGAATCCATCCTTCAAGTAGGACTGATAGAGGTGTACATGCGATACAGCAATAC
    TTTCATTTCGACACAGAGCTGAATATACCTATGTCACAATGGCAATATGCAATGAACCGA
    ACGTTACCTGATGATATTTATGTCAACAATGTAGTTACAGTCGATGACGATTTTCATTGT
    CGTTATGATTGTGTAGGTAAACGTTATCGCTACAAAGTATATCAAGCACAACACCGCGAT
    CCATTTCAAAGTGGTTTGAAAACATTTATTCCTGAAACGTTGGATTTAGGTAAAATGAAC
    AGAGCGGCGCAACAATTCATAGGTACACATGATTTTACGGGATTTTGTTCGCAAAAAACT
    GAAGTAGAAAGCAAAGTTAGAACATTATACCAAAGTGAAATCGTAAAGACAGATGATGGC
    TTTGATTATATTGTCACTGGATCTGGATTTTTATATAACATGGTGCGTGTCTTAGTTGCA
    TTTTTAATAGAAGTTGGAAAAGGGCGACATGAAGTATCTGATGTACCAAAACTTCTTGAA
    TCAAAGAATCGTAAGAATGTACCTTTCACTGCACCGGCTGAAGGATTATATTTAGAGAAA
    ATTTATTTAGATGAAAATGAGTTATTAAAAGACTTTGGTAACGATATCAAAATACATCGT
    AAAAAATCATTACAAAATGACTGA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    804


⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL2208 [new locus tag: SACOL_RS11625 ]
  • symbol: TruA
  • description: tRNA pseudouridine synthase A
  • length: 267
  • theoretical pI: 8.70635
  • theoretical MW: 31279.3
  • GRAVY: -0.591386

⊟Function[edit | edit source]

  • reaction:
    EC 5.4.99.12?  ExPASy
    tRNA pseudouridine(38-40) synthase tRNA uridine(38-40) = tRNA pseudouridine(38-40)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA and rRNA base modification tRNA pseudouridine(38-40) synthase (TIGR00071; EC 5.4.99.12; HMM-score: 219.2)
    and 1 more
    radical SAM-linked protein (TIGR03936; HMM-score: 16.8)
  • TheSEED  :
    • tRNA pseudouridine synthase A (EC 4.2.1.70)
    RNA Metabolism RNA processing and modification tRNA modification Archaea  tRNA pseudouridine synthase A (EC 4.2.1.70)
    and 2 more
    RNA Metabolism RNA processing and modification tRNA processing  tRNA pseudouridine synthase A (EC 4.2.1.70)
    Virulence, Disease and Defense Bacteriocins, ribosomally synthesized antibacterial peptides Colicin V and Bacteriocin Production Cluster  tRNA pseudouridine synthase A (EC 4.2.1.70)
  • PFAM:
    PseudoU_synth (CL0649) PseudoU_synth_1; tRNA pseudouridine synthase (PF01416; HMM-score: 148.8)
    and 2 more
    AAA_lid (CL0671) AAA_lid_6; AAA lid domain (PF17866; HMM-score: 15.9)
    PseudoU_synth (CL0649) DUF2344; Uncharacterized protein conserved in bacteria (DUF2344) (PF10105; HMM-score: 14.4)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • DeepLocPro: Cytoplasmic
    • Cytoplasmic Score: 0.9723
    • Cytoplasmic Membrane Score: 0.027
    • Cell wall & surface Score: 0.0001
    • Extracellular Score: 0.0005
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.002749
    • TAT(Tat/SPI): 0.000193
    • LIPO(Sec/SPII): 0.000467
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRIHPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRYDCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLGKMNRAAQQFIGTHDFTGFCSQKTEVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKLLESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 59 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Additional information (user-provided)[edit | edit source]

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Additional information (user-provided)[edit | edit source]

⊟Other information (user-provided)[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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