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NCBI: 10-JUN-2013
⊟Summary[edit | edit source]
- organism: Staphylococcus aureus COL
- locus tag: SACOL1444 [new locus tag: SACOL_RS07365 ]
- pan locus tag?: SAUPAN003826000
- symbol: SACOL1444
- pan gene symbol?: —
- synonym:
- product: hypothetical protein
⊟Genome View[edit | edit source]
⊟Gene[edit | edit source]
⊟General[edit | edit source]
- type: CDS
- locus tag: SACOL1444 [new locus tag: SACOL_RS07365 ]
- symbol: SACOL1444
- product: hypothetical protein
- replicon: chromosome
- strand: -
- coordinates: 1455198..1457084
- length: 1887
- essential: unknown other strains
⊟Accession numbers[edit | edit source]
- Gene ID: 3236552 NCBI
- RefSeq: YP_186296 NCBI
- BioCyc: see SACOL_RS07365
- MicrobesOnline: 912902 MicrobesOnline
⊟Phenotype[edit | edit source]
Share your knowledge and add information here. [edit]
⊟DNA sequence[edit | edit source]
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1861ATGAGTGATCGTTTCATAAAATTTAATGACGAACAGTTAGATGCCAAGCAAGTAATGATG
TTGCAAGACTTAGCTCGACTCTTATTAAAAAATGAACAAACACAAGTTAAAATCCAAAAA
TTCCCTTACTATAATCCTGTTCAAAATGTTTTGATTACGAGTTGGTTTTGGTCACATCGA
CCAAGCCATATCGAAATGGCTGGTTTAAAAACAGATGTTATGTTAGCTGCTTACGGTTAT
CATATGATGGATGTACAAATTGTAAATGAAGTAGTACAAGATAAAACTTTTAAACACCCT
AAATTTTATCAACAACTTTTTAAATTATTAGAAGATATGCGCGTATTAAATTCTATTAAA
GTTGAGCGCCCAAGTACTGCTAAATTAATTGATCTTCGCTTAGATACACGTATATCATAT
ACGGAATCTCAAATTAAAGTGTATCGTACTAAAACACAATATACAGATTTATTATTTTTG
TATTTAGAACATGCATTTTTAAGCCAAGATTTCTTTGATATACCATCTATACATTCAGAT
TTAGATGATATTTTAGTAAATATGTTCTTATATTTACCTAATTTTTTCCAAAATCAAAAT
TCTGAAGATAATATGTATCTGGCTCAAAGAATTATGTATCAAGTAGATGATATTTTAAAA
GAAGATATGCTAAACGAATATTATTACTTACCTAAAACACTATATAATACATTAGCTAGT
CCAGAGTTTGATGATTTAAAGAGAACTGATGCAAGTCAAGTCGATGGGCAAGATGATACT
TCAGAAGATGATGATAACGAAAGTGAAAAAGCTGACTCTAAATCTGCAGATAGTGAGTCA
AAAGGTGGCGCCTATTTAGAAATGGAGCTTCACGAAGGACAAAATAGTGAAACATTAGGT
AATGATGAAGCACGTGAAGGCGATGCTACAGATGATATGACTGATATGATGACCAAAAAG
GGCAAAGGATCAAACGATACGCTAAATCGTGAAGAAGGCGATGCTGTTGGTCAAAGCCAA
GCATTTCAACTTGATGGTGTGAACAAAAATGTAGAAATAAAATGGCAAATTCCTGAAATT
GAGCCACAATATGTTTTAGAATATCAAGAATCGAAACAAGACGTACAATATGAAATCAAA
GACTTAATTCAAATTATAAAGAAAACGATTGAACGAGAACAACGAGATGCACGTTTTAAT
TTAACAAAAGGGCGATTACAAAAGGATTTAATTAATTGGTTTATCGACGATCAATATAAA
TTGTTTTACAAAAAACAAGATTTAAGTAAATCATTTGATGCGACATTTACATTGTTAATT
GATGCGTCAGCAAGTATGCATGATAAAATGGCTGAAACGAAGAAAGGTGTAGTGTTATTC
CACGAGACGTTAAAAGCATTGAACATTAAACATGAAATTTTATCATTCAGTGAGGATGCC
TTTGATTCAGACGAGCATGCACAACCAAATATCATTAATGAAATTATTAACTATGATTAC
TCAACCTTTGAAAAAGATGGCCCACGTATTATGGCACTTGAACCTCAAGATGATAATCGT
GATGGTGTTGCTATTAGAGTTGCAAGTGAACGATTAATGCGTCGAAATCAACATCAACGA
TTTTTAATCGTATTTTCAGACGGCGAACCGTCTGCATTTAATTATAGTCAAGATGGTATT
ATTGATACGTATGAAGCTGTAGAAATGTCACGTAAATTTGGTATTGAAGTATTTAATGTA
TTTTTGAGTCAAGATCCAATAACTGAAGATGTTGAACAAACAATTCATAATATTTATGGT
CAATATGCTATTTTCGTTGAAGGTGTTGCGCATTTACCTGGACATCTCTCTCCATTATTA
AAAAAATTACTACTTAAATCTTTATAA60
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⊟Protein[edit | edit source]
⊟General[edit | edit source]
- locus tag: SACOL1444 [new locus tag: SACOL_RS07365 ]
- symbol: SACOL1444
- description: hypothetical protein
- length: 628
- theoretical pI: 4.50167
- theoretical MW: 73099.7
- GRAVY: -0.581051
⊟Function[edit | edit source]
- TIGRFAM: Biosynthesis of cofactors, prosthetic groups, and carriers Heme, porphyrin, and cobalamin cobaltochelatase, CobT subunit (TIGR01651; EC 6.6.1.2; HMM-score: 21.5)
- TheSEED :
- Nitric oxide reductase activation protein NorD
- PFAM: vWA-like (CL0128) CobT_C; Cobalamin biosynthesis protein CobT VWA domain (PF11775; HMM-score: 25.1)VWA; von Willebrand factor type A domain (PF00092; HMM-score: 25)VWA_2; von Willebrand factor type A domain (PF13519; HMM-score: 21.5)and 1 moreHoming_endonuc (CL0324) LAGLIDADG_1; LAGLIDADG endonuclease (PF00961; HMM-score: 14)
⊟Structure, modifications & cofactors[edit | edit source]
- domains:
- modifications:
- cofactors:
- effectors:
⊟Localization[edit | edit source]
- PSORTb: Cytoplasmic
- Cytoplasmic Score: 7.5
- Cytoplasmic Membrane Score: 1.15
- Cellwall Score: 0.62
- Extracellular Score: 0.73
- Internal Helices: 0
- LocateP: Intracellular
- Prediction by SwissProt Classification: Cytoplasmic
- Pathway Prediction: No pathway
- Intracellular possibility: 1
- Signal peptide possibility: -1
- N-terminally Anchored Score: 1
- Predicted Cleavage Site: No CleavageSite
- SignalP: no predicted signal peptide
- SP(Sec/SPI): 0.001082
- TAT(Tat/SPI): 0.000433
- LIPO(Sec/SPII): 0.000245
- predicted transmembrane helices (TMHMM): 0
⊟Accession numbers[edit | edit source]
⊟Protein sequence[edit | edit source]
- MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYYNPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKFYQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRISYTESQIKVYRTKTQYTDLLFLYLEHAFLSQDFFDIPSIHSDLDDILVNMFLYLPNFFQNQNSEDNMYLAQRIMYQVDDILKEDMLNEYYYLPKTLYNTLASPEFDDLKRTDASQVDGQDDTSEDDDNESEKADSKSADSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGDAVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIEREQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKMAETKKGVVLFHETLKALNIKHEILSFSEDAFDSDEHAQPNIINEIINYDYSTFEKDGPRIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEAVEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLLLKSL
⊟Experimental data[edit | edit source]
- experimentally validated: PeptideAtlas
- protein localization: Cytoplasmic [1] [2] [3]
- quantitative data / protein copy number per cell: 76 [4]
- interaction partners:
⊟Expression & Regulation[edit | edit source]
⊟Operon[edit | edit source]
⊟Regulation[edit | edit source]
- regulator:
⊟Transcription pattern[edit | edit source]
- S.aureus Expression Data Browser: data available for NCTC8325
⊟Protein synthesis (provided by Aureolib)[edit | edit source]
- Aureolib: no data available
⊟Protein stability[edit | edit source]
- half-life: no data available
⊟Biological Material[edit | edit source]
⊟Mutants[edit | edit source]
⊟Expression vector[edit | edit source]
⊟lacZ fusion[edit | edit source]
⊟GFP fusion[edit | edit source]
⊟two-hybrid system[edit | edit source]
⊟FLAG-tag construct[edit | edit source]
⊟Antibody[edit | edit source]
⊟Other Information[edit | edit source]
You are kindly invited to share additional interesting facts.
⊟Literature[edit | edit source]
⊟References[edit | edit source]
- ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
PLoS One: 2009, 4(12);e8176
[PubMed:19997597] [WorldCat.org] [DOI] (I e) - ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
J Proteome Res: 2011, 10(4);1657-66
[PubMed:21323324] [WorldCat.org] [DOI] (I p) - ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
The Staphylococcus aureus proteome.
Int J Med Microbiol: 2014, 304(2);110-20
[PubMed:24439828] [WorldCat.org] [DOI] (I p) - ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
Sci Rep: 2016, 6;28172
[PubMed:27344979] [WorldCat.org] [DOI] (I e)