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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL1223 [new locus tag: SACOL_RS06265 ]
  • pan locus tag?: SAUPAN003493000
  • symbol: coaBC
  • pan gene symbol?: coaBC
  • synonym:
  • product: phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL1223 [new locus tag: SACOL_RS06265 ]
  • symbol: coaBC
  • product: phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase
  • replicon: chromosome
  • strand: +
  • coordinates: 1231668..1232873
  • length: 1206
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    TTGAATATGAAGAAAATATTATTAGCCGTTACAGGTGGCATTGCGGCATATAAAGCAATT
    GATTTGACAAGTAAGTTAACACAATCTGGGTATGAAGTTCGCGTTATGTTAACGAATCAC
    GCACAAAAATTTGTGACACCATTAGCATTTCAAGCAATAAGTCGAAATGCTGTTTATACA
    GATACTTTTATAGAAGAAAATCCTTCAGAAATACAGCATATTGCATTAGGTGATTGGGCA
    GATGCAATCATTGTTGCACCTGCAACGGCAAATACAATTGCAAAATTGAGTGTAGGTATT
    GCTGATGATTTGGTGACATCAACGTTGCTAGCAACAGAGACACCGAAATTTATTGCGCCT
    GCTATGAATGTGCATATGTATGAAAATAAACGTACGCAGCAAAATATTAATATTTTAAAA
    GAAGATGGGTATCATTTTATCGAACCAGGAAGCGGATTTCTAGCATGTGGTTATGTTGCT
    AAAGGACGTATGGAAGAACCGCTTCAAATCGTTTCTGTAATTGATGCTCATTTTCAAAAT
    AGTAATCGTTTAGCTAATAGTTCATTTCAAGATAAACGCGCATTGGTTACAGCAGGACCA
    ACTATTGAAGTTATCGATCCAGTCAGATTTGTATCCAATCGTTCTTCTGGAAAAATGGGC
    TATGCAATAGCTGAAGCATTGCGAAATCGAGGAGCTATCGTGACGTTAGTTGCTGGTCCT
    ACAACACTAGAGGATCCAAAAGATATTGAAGTTATTCATGTTCAAAGTGCTGAAGAAATG
    TTTGAACAAGTGACAAGCCGATTTGACGAACAAGATATTGTTGTAAAAGCAGCAGCCGTA
    TCTGACTATACACCGATTGATGTATTAGAACATAAGATGAAAAAGCAGGATGGTGATTTG
    TCAGTATCTTTTAAACGTACTAAAGACATTCTTAAATATTTAGGTGAACATAAAACATCA
    CAGTATTTAATAGGCTTTGCAGCAGAGACTGAAGATATTGAAAATTATGCACAACAAAAA
    TTACGCAAGAAAAATGCAGATGTGATTATTTCAAATAATGTTGGGGATATGTCTATCGGA
    TTTAGTTCTGATGATAATGAATTGACAATGCATTTTAAAAATAATGAAAAAGTAAATATC
    AAGAAAGGAAAAAAAGTAGTATTAGCTGCACAAATTTTAGATGAACTAGAAACTAGGTGG
    CAATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1206

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL1223 [new locus tag: SACOL_RS06265 ]
  • symbol: CoaBC
  • description: phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase
  • length: 401
  • theoretical pI: 5.87138
  • theoretical MW: 44399.3
  • GRAVY: -0.213466

⊟Function[edit | edit source]

  • reaction:
    EC 6.3.2.5?  ExPASy
    Phosphopantothenate--cysteine ligase (CTP) CTP + (R)-4'-phosphopantothenate + L-cysteine = CMP + diphosphate + N-((R)-4'-phosphopantothenoyl)-L-cysteine
    EC 4.1.1.36?  ExPASy
    Phosphopantothenoylcysteine decarboxylase N-((R)-4'-phosphopantothenoyl)-L-cysteine = pantotheine 4'-phosphate + CO2
  • TIGRFAM:
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A phosphopantothenoylcysteine decarboxylase / phosphopantothenate--cysteine ligase (TIGR00521; EC 4.1.1.36,6.3.2.5; HMM-score: 477.7)
    and 6 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A phosphopantothenoylcysteine decarboxylase (TIGR02113; EC 4.1.1.36; HMM-score: 208.5)
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Pantothenate and coenzyme A phosphopantothenate--cysteine ligase (TIGR02114; EC 6.3.2.5; HMM-score: 70.9)
    Metabolism Energy metabolism Electron transport archaeoflavoprotein AfpA (TIGR02699; HMM-score: 32.3)
    Unknown function General archaeoflavoprotein, MJ0208 family (TIGR02700; HMM-score: 31.4)
    Unknown function Enzymes of unknown specificity polyprenyl P-hydroxybenzoate and phenylacrylic acid decarboxylases (TIGR00421; EC 4.1.1.-; HMM-score: 27.7)
    Cellular processes Cellular processes Sporulation and germination dipicolinic acid synthetase, B subunit (TIGR02852; HMM-score: 16.4)
  • TheSEED  :
    • Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36)
    • Phosphopantothenoylcysteine synthetase (EC 6.3.2.5)
    Cofactors, Vitamins, Prosthetic Groups, Pigments Coenzyme A Coenzyme A Biosynthesis  Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36)
    and 1 more
    Cofactors, Vitamins, Prosthetic Groups, Pigments Coenzyme A Coenzyme A Biosynthesis  Phosphopantothenoylcysteine synthetase (EC 6.3.2.5)
  • PFAM:
    NADP_Rossmann (CL0063) DFP; DNA / pantothenate metabolism flavoprotein (PF04127; HMM-score: 232.6)
    and 4 more
    no clan defined Flavoprotein; Flavoprotein (PF02441; HMM-score: 161.6)
    NADP_Rossmann (CL0063) YjeF_N; YjeF-related protein N-terminus (PF03853; HMM-score: 17.9)
    adh_short_C2; Enoyl-(Acyl carrier protein) reductase (PF13561; HMM-score: 16.1)
    SPFH (CL0433) Band_7; SPFH domain / Band 7 family (PF01145; HMM-score: 13.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: FMN
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 0.83
    • Signal peptide possibility: -0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.15503
    • TAT(Tat/SPI): 0.001893
    • LIPO(Sec/SPII): 0.052178
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MNMKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVTPLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDLVTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKGRMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMGYAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAAAVSDYTPIDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENYAQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILDELETRWQ

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3] [4]
  • quantitative data / protein copy number per cell: 85 [5]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Jan Pané-Farré, Andreas Otto, Susanne Sievers, Michael Hecker, Dörte Becher
    Quantitative cell surface proteome profiling for SigB-dependent protein expression in the human pathogen Staphylococcus aureus via biotinylation approach.
    J Proteome Res: 2010, 9(3);1579-90
    [PubMed:20108986] [WorldCat.org] [DOI] (I p)
  3. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  4. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  5. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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