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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0762 [new locus tag: SACOL_RS03915 ]
  • pan locus tag?: SAUPAN002587000
  • symbol: SACOL0762
  • pan gene symbol?: mpfA
  • synonym:
  • product: hemolysin

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0762 [new locus tag: SACOL_RS03915 ]
  • symbol: SACOL0762
  • product: hemolysin
  • replicon: chromosome
  • strand: +
  • coordinates: 784134..785483
  • length: 1350
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    TTGGAAACTTCGACCATAATTAGTTTGATTATATTTATTCTATTAATTGCATTAACCACT
    GTATTTGTTGGTTCAGAATTTGCATTAGTAAAAATTAGAGCAACAAGAATTGAACAGCTA
    GCAGATGAAGGAAATAAACCTGCTAAAATAGTAAAAAAGATGATTGCTAATCTAGATTAT
    TATCTTTCTGCTTGTCAGTTAGGTATAACAGTAACATCTTTAGGGTTAGGTTGGCTTGGT
    GAACCAACGTTTGAAAAGCTATTACACCCAATATTTGAAGCAATCAATTTACCAACTGCA
    TTAACGACGACGATTTCGTTTGCAGTGTCATTTATAATCGTTACGTATTTGCATGTAGTA
    CTTGGTGAATTAGCGCCTAAATCTATAGCTATTCAACATACTGAAAAGCTTGCTTTAGTA
    TATGCAAGACCATTGTTCTATTTCGGTAACATTATGAAACCATTGATTTGGCTGATGAAT
    GGTTCTGCACGTGTTATTATTAGAATGTTTGGTGTAAATCCTGATGCCCAAACTGATGCA
    ATGTCAGAAGAAGAAATCAAAATTATTATTAACAATAGTTATAATGGTGGAGAAATCAAC
    CAAACTGAATTGGCATATATGCAAAATATCTTTTCATTCGATGAAAGACATGCAAAAGAT
    ATAATGGTACCTAGAACTCAAATGATTACACTAAATGAACCTTTTAATGTAGACGAATTA
    CTAGAAACAATAAAAGAACATCAATTTACGCGTTATCCAATTACTGATGATGGTGATAAA
    GACCACATTAAAGGATTTATTAACGTCAAAGAATTTTTAACTGAATACGCTTCTGGAAAA
    ACGATTAAAATAGCAAACTATATACATGAGTTGCCAATGATTTCAGAGACAACACGTATC
    AGTGATGCATTAATTAGAATGCAACGTGAACATGTACATATGAGTCTTATTATAGATGAA
    TATGGTGGAACGGCAGGTATTTTAACGATGGAAGATATTTTAGAAGAAATCGTTGGAGAA
    ATTCGTGATGAATTTGATGATGATGAAGTGAATGATATCGTTAAAATTGATAATAAGACA
    TTCCAAGTAAATGGCAGAGTACTATTGGATGATTTAACTGAAGAGTTCGGTATAGAATTT
    GATGACTCTGAGGATATTGATACGATAGGTGGATGGTTACAATCTCGTAATACCAATTTA
    CAAAAAGATGATTACGTGGATACAACTTATGATCGCTGGGTTGTTTCAGAAATCGATAAC
    CACCAAATTATTTGGGTGATATTAAACTATGAATTTAATGAAGCGAGACCTACTATCGGA
    CAGTCTGATGAAGATGAAAAATCAGAATAG
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1350

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0762 [new locus tag: SACOL_RS03915 ]
  • symbol: SACOL0762
  • description: hemolysin
  • length: 449
  • theoretical pI: 4.25784
  • theoretical MW: 51150
  • GRAVY: -0.0665924

⊟Function[edit | edit source]

  • TIGRFAM:
    gliding motility-associated protein GldE (TIGR03520; HMM-score: 172.3)
    and 2 more
    Metabolism Energy metabolism Sugars sugar isomerase, KpsF/GutQ family (TIGR00393; HMM-score: 24.8)
    Metabolism Purines, pyrimidines, nucleosides, and nucleotides Purine ribonucleotide biosynthesis inosine-5'-monophosphate dehydrogenase (TIGR01302; EC 1.1.1.205; HMM-score: 23.6)
  • TheSEED  :
    • Magnesium exporter MpfA
    Phosphorus Metabolism Phosphorus Metabolism - no subcategory Phosphate metabolism  Magnesium and cobalt efflux protein CorC
  • PFAM:
    no clan defined CNNM; Cyclin M transmembrane N-terminal domain (PF01595; HMM-score: 185.5)
    and 3 more
    CorC_HlyC; Transporter associated domain (PF03471; HMM-score: 58.8)
    CBS; CBS domain (PF00571; HMM-score: 57.9)
    MGS; MGS-like domain (PF02142; HMM-score: 13.6)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 10
    • Cellwall Score: 0
    • Extracellular Score: 0
    • Internal Helices: 3
  • DeepLocPro: Cytoplasmic Membrane
    • Cytoplasmic Score: 0
    • Cytoplasmic Membrane Score: 1
    • Cell wall & surface Score: 0
    • Extracellular Score: 0
  • LocateP: Multi-transmembrane(Lipid modified N-termini)
    • Prediction by SwissProt Classification: Membrane
    • Pathway Prediction: Sec-(SPII)
    • Intracellular possibility: 0.17
    • Signal peptide possibility: 0.5
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: YLSACQL
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.311499
    • TAT(Tat/SPI): 0.003521
    • LIPO(Sec/SPII): 0.039307
  • predicted transmembrane helices (TMHMM): 4

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEGNKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALTTTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMNGSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHAKDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEYASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDILEEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGWLQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVILNYEFNEARPTIGQSDEDEKSE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Integral membrane [1] [2] [3]
  • quantitative data / protein copy number per cell:
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You can add further information about the gene and protein here. [edit]

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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