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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0576 [new locus tag: SACOL_RS02990 ]
  • pan locus tag?: SAUPAN002298000
  • symbol: cysS
  • pan gene symbol?: cysS
  • synonym:
  • product: cysteinyl-tRNA synthetase

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0576 [new locus tag: SACOL_RS02990 ]
  • symbol: cysS
  • product: cysteinyl-tRNA synthetase
  • replicon: chromosome
  • strand: +
  • coordinates: 598522..599922
  • length: 1401
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    ATGATTACATTATATAATACGCTTACACGTCAAAAAGAAGTGTTCAAGCCTATAGAACCA
    GGGAAAGTAAAAATGTATGTATGTGGTCCTACTGTATATAACTACATTCATATTGGTAAC
    GCAAGACCAGCAATTAATTATGACGTAGTGAGACGTTACTTTGAATACCAAGGATATAAT
    GTAGAATATGTATCAAATTTTACAGACGTAGATGATAAATTAATTAAACGTTCTCAAGAA
    TTAAATCAGTCTGTTCCCGAAATTGCAGAAAAATATATCGCTGCTTTTCATGAAGATGTT
    GGTGCGTTAAATGTTAGAAAAGCGACTTCAAATCCAAGGGTAATGGACCATATGGATGAC
    ATTATTCAATTTATTAAAGATTTGGTGGATCAAGGTTATGCATATGAAAGTGGTGGCGAT
    GTTTACTTTAGAACACGTAAATTTGAAGGTTATGGTAAATTAAGTCATCAATCCATAGAT
    GACTTAAAAGTGGGTGCTCGTATAGATGCAGGAGAGCATAAAGAAGATGCACTTGATTTT
    ACATTGTGGAAAAAAGCGAAGCCTGGCGAGATTAGTTGGGATAGCCCATTTGGTGAAGGT
    AGACCAGGATGGCATATAGAATGTTCTGTAATGGCATTTCATGAGCTAGGACCTACAATT
    GATATACATGCGGGTGGTTCAGATTTACAATTTCCACATCATGAAAATGAAATAGCACAA
    TCAGAAGCACATAATCATGCGCCATTTGCTAATTATTGGATGCATAATGGTTTCATTAAT
    ATTGATAATGAAAAAATGAGTAAATCACTAGGCAACTTTATTTTAGTTCACGATATTATT
    AAAGAAGTTGATCCAGATGTACTAAGATTCTTTATGATTAGCGTACATTATAGAAGCCCA
    ATTAACTATAATCTAGAATTGGTAGAATCAGCACGTAGTGGACTAGAGCGTATTCGCAAT
    AGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACA
    TATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTT
    AATACAGCTAATGCAATTACAGCTTGGTATGATTTAGCAAAACTTGCGAATAAATATGTA
    CTAGAGAACACAACATCAACAGAAGTAATTGATAAATTTAAAGCAGTTTATCAAATTTTC
    AGCGATGTTTTAGGTGTACCGTTAAAATCTAAAAATGCAGATGAATTATTGGATGAAGAT
    GTTGAAAAATTAATCGAAGAGCGTAATGAAGCAAGGAAAAACAAAGATTTTGCACGAGCA
    GATGAAATTCGAGACATGCTGAAATCACAAAACATTATATTAGAAGACACACCTCAAGGG
    GTTAGATTTAAACGTGGATAA
    60
    120
    180
    240
    300
    360
    420
    480
    540
    600
    660
    720
    780
    840
    900
    960
    1020
    1080
    1140
    1200
    1260
    1320
    1380
    1401

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0576 [new locus tag: SACOL_RS02990 ]
  • symbol: CysS
  • description: cysteinyl-tRNA synthetase
  • length: 466
  • theoretical pI: 5.03617
  • theoretical MW: 53684.9
  • GRAVY: -0.524678

⊟Function[edit | edit source]

  • reaction:
    EC 6.1.1.16?  ExPASy
    Cysteine--tRNA ligase ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)
  • TIGRFAM:
    Genetic information processing Protein synthesis tRNA aminoacylation cysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 594.1)
    and 7 more
    Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs cysteine--1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (TIGR03447; EC 6.3.1.13; HMM-score: 271)
    Genetic information processing Protein synthesis tRNA aminoacylation methionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 59.5)
    Genetic information processing Protein synthesis tRNA aminoacylation isoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 46.1)
    Genetic information processing Protein synthesis tRNA aminoacylation valine--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 41.7)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 38.1)
    Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00467; EC 6.1.1.6; HMM-score: 28.2)
    Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 27.2)
  • TheSEED  :
    • Cysteinyl-tRNA synthetase (EC 6.1.1.16)
    Miscellaneous Plant-Prokaryote comparative genomics Conserved gene cluster possibly involved in RNA metabolism  Cysteinyl-tRNA synthetase (EC 6.1.1.16)
  • PFAM:
    HUP (CL0039) tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 441.7)
    and 6 more
    DALR (CL0258) DALR_2; DALR domain (PF09190; HMM-score: 55.8)
    HUP (CL0039) tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 53.9)
    tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 41.1)
    tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 38)
    no clan defined PP2C_C; Protein serine/threonine phosphatase 2C, C-terminal domain (PF07830; HMM-score: 12.8)
    UVR; UvrB/uvrC motif (PF02151; HMM-score: 12.1)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors: Zn2+
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 9.97
    • Cytoplasmic Membrane Score: 0
    • Cellwall Score: 0.01
    • Extracellular Score: 0.02
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.063455
    • TAT(Tat/SPI): 0.000583
    • LIPO(Sec/SPII): 0.004528
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPAINYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGALNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSIDDLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGPTIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILVHDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATNIENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDKFKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQNIILEDTPQGVRFKRG

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1] [2] [3]
  • quantitative data / protein copy number per cell: 611 [4]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator: T-box(Cys) (transcription antitermination) regulon
    T-box(Cys)(RNA)important in Amino acid metabolism; transcription unit transferred from N315 data RegPrecise 

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: 48.01 h [5]

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Dörte Becher, Kristina Hempel, Susanne Sievers, Daniela Zühlke, Jan Pané-Farré, Andreas Otto, Stephan Fuchs, Dirk Albrecht, Jörg Bernhardt, Susanne Engelmann, Uwe Völker, Jan Maarten van Dijl, Michael Hecker
    A proteomic view of an important human pathogen--towards the quantification of the entire Staphylococcus aureus proteome.
    PLoS One: 2009, 4(12);e8176
    [PubMed:19997597] [WorldCat.org] [DOI] (I e)
  2. ↑ Kristina Hempel, Florian-Alexander Herbst, Martin Moche, Michael Hecker, Dörte Becher
    Quantitative proteomic view on secreted, cell surface-associated, and cytoplasmic proteins of the methicillin-resistant human pathogen Staphylococcus aureus under iron-limited conditions.
    J Proteome Res: 2011, 10(4);1657-66
    [PubMed:21323324] [WorldCat.org] [DOI] (I p)
  3. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  4. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)
  5. ↑ Stephan Michalik, Jörg Bernhardt, Andreas Otto, Martin Moche, Dörte Becher, Hanna Meyer, Michael Lalk, Claudia Schurmann, Rabea Schlüter, Holger Kock, Ulf Gerth, Michael Hecker
    Life and death of proteins: a case study of glucose-starved Staphylococcus aureus.
    Mol Cell Proteomics: 2012, 11(9);558-70
    [PubMed:22556279] [WorldCat.org] [DOI] (I p)

⊟Relevant publications[edit | edit source]

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