PangenomeCOLN315NCTC8325NewmanUSA300_FPR375704-0298108BA0217611819-97685071193ECT-R 2ED133ED98HO 5096 0412JH1JH9JKD6008JKD6159LGA251M013MRSA252MSHR1132MSSA476MW2Mu3Mu50RF122ST398T0131TCH60TW20USA300_TCH1516VC40

NCBI: 26-AUG-2013

⊟Summary

• organism: Staphylococcus aureus N315
• locus tag: SA0488 [new locus tag: SA_RS02870 ]
• pan locus tag^?: SAUPAN002298000
• symbol: cysS
• pan gene symbol^?: cysS
• synonym:
• product: cysteinyl-tRNA synthetase

⊟Genome View

⊟Gene

⊟General

• type: CDS
• locus tag: SA0488 [new locus tag: SA_RS02870 ]
• symbol: cysS
• product: cysteinyl-tRNA synthetase
• replicon: chromosome
• strand: +
• coordinates: 571154..572554
• length: 1401
• essential: yes DEG other strains

⊟Accession numbers

• Gene ID: 1123292 NCBI
• RefSeq: NP_373740 NCBI
• BioCyc: see SA_RS02870
• MicrobesOnline: 102766 MicrobesOnline

⊟Phenotype

Share your knowledge and add information here. [edit]

⊟DNA sequence

• 1
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  ATGATTACATTATATAATACGCTTACACGTCAAAAAGAAGTGTTCAAGCCTATAGAACCA
  GGGAAAGTAAAAATGTATGTATGTGGTCCTACTGTATATAACTACATTCATATTGGTAAC
  GCAAGACCAGCAATTAATTATGACGTAGTGAGACGTTACTTTGAATACCAAGGATATAAT
  GTAGAATATGTATCAAATTTTACAGACGTAGATGATAAATTAATTAAACGTTCTCAAGAA
  TTAAATCAGTCTGTTCCCGAAATTGCAGAAAAATATATCGCTGCTTTTCATGAAGATGTT
  GGTGCGTTAAATGTTAGAAAAGCGACTTCAAATCCAAGGGTAATGGACCATATGGATGAC
  ATTATTCAATTTATTAAAGATTTGGTGGATCAAGGTTATGCATATGAAAGTGGTGGCGAT
  GTTTACTTTAGAACACGTAAATTTGAAGGTTATGGTAAATTAAGTCATCAATCCATAGAT
  GACTTAAAAGTGGGTGCTCGTATAGATGCAGGAGAGCATAAAGAAGATGCACTTGATTTT
  ACATTGTGGAAAAAAGCGAAGCCTGGCGAGATTAGTTGGAATAGCCCATTTGGTGAAGGT
  AGACCAGGATGGCATATAGAATGTTCTGTAATGGCATTTCATGAGCTAGGACCTACAATT
  GATATACATGCGGGTGGTTCAGATTTACAATTTCCACATCATGAAAATGAAATAGCACAA
  TCAGAAGCACATAATCATGCGCCATTTGCTAATTATTGGATGCATAATGGTTTCATTAAT
  ATTGATAATGAAAAAATGAGTAAATCACTAGGCAACTTTATTTTAGTTCACGATATTATT
  AAAGAAGTTGATCCAGATGTACTAAGATTCTTTATGATTAGCGTACATTATAGAAGCCCA
  ATTAACTATAATCTAGAATTGGTAGAATCAGCACGTAGTGGACTAGAGCGTATTCGCAAT
  AGTTATCAATTAATTGAAGAGCGCGCACAAATTGCTACTAATATTGAAAATCAACAGACA
  TATATTGATCAAATTGATGCGATTTTAAATCGTTTTGAAACAGTTATGAATGATGATTTT
  AATACAGCTAATGCAATTACAGCTTGGTATGATTTAGCAAAACTTGCGAATAAATATGTA
  CTAGAGAACACAACATCAACAGAAGTAATTGATAAATTTAAAGCAGTTTATCAAATTTTC
  AGCGATGTTTTAGGTGTACCGTTAAAATCTAAAAATGCAGATGAATTATTGGATGAAGAT
  GTTGAAAAATTAATCGAAGAGCGTAATGAAGCAAGGAAAAACAAAGATTTTGCACGAGCA
  GATGAGATTCGAGACATGCTGAAATCACAAAACATTATATTAGAAGACACACCTCAAGGG
  GTTAGATTTAAACGTGGATAA
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⊟Protein

⊟General

• locus tag: SA0488 [new locus tag: SA_RS02870 ]
• symbol: CysS
• description: cysteinyl-tRNA synthetase
• length: 466
• theoretical pI: 5.10346
• theoretical MW: 53684
• GRAVY: -0.524678

⊟Function

• reaction:
  EC 6.1.1.16?  ExPASy
  Cysteine--tRNA ligase ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys)
• TIGRFAM:
  Genetic information processing Protein synthesis tRNA aminoacylation cysteine--tRNA ligase (TIGR00435; EC 6.1.1.16; HMM-score: 593.5)
  and 7 more
  Metabolism Biosynthesis of cofactors, prosthetic groups, and carriers Glutathione and analogs cysteine--1-D-myo-inosityl 2-amino-2-deoxy-alpha-D-glucopyranoside ligase (TIGR03447; EC 6.3.1.13; HMM-score: 270.1)
  Genetic information processing Protein synthesis tRNA aminoacylation methionine--tRNA ligase (TIGR00398; EC 6.1.1.10; HMM-score: 59.5)
  Genetic information processing Protein synthesis tRNA aminoacylation isoleucine--tRNA ligase (TIGR00392; EC 6.1.1.5; HMM-score: 46.1)
  Genetic information processing Protein synthesis tRNA aminoacylation valine--tRNA ligase (TIGR00422; EC 6.1.1.9; HMM-score: 41.7)
  Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00395; EC 6.1.1.4; HMM-score: 38.1)
  Genetic information processing Protein synthesis tRNA aminoacylation lysine--tRNA ligase (TIGR00467; EC 6.1.1.6; HMM-score: 27.5)
  Genetic information processing Protein synthesis tRNA aminoacylation leucine--tRNA ligase (TIGR00396; EC 6.1.1.4; HMM-score: 27.2)
• TheSEED  :
  • Cysteinyl-tRNA synthetase (EC 6.1.1.16)
  Miscellaneous Plant-Prokaryote comparative genomics Conserved gene cluster possibly involved in RNA metabolism  Cysteinyl-tRNA synthetase (EC 6.1.1.16)
• PFAM:
  HUP (CL0039) tRNA-synt_1e; tRNA synthetases class I (C) catalytic domain (PF01406; HMM-score: 441)
  and 6 more
  DALR (CL0258) DALR_2; DALR domain (PF09190; HMM-score: 55.8)
  HUP (CL0039) tRNA-synt_1g; tRNA synthetases class I (M) (PF09334; HMM-score: 53.9)
  tRNA-synt_1; tRNA synthetases class I (I, L, M and V) (PF00133; HMM-score: 41.1)
  tRNA-synt_1f; tRNA synthetases class I (K) (PF01921; HMM-score: 38)
  no clan defined PP2C_C; Protein serine/threonine phosphatase 2C, C-terminal domain (PF07830; HMM-score: 12.8)
  UVR; UvrB/uvrC motif (PF02151; HMM-score: 12.1)

⊟Structure, modifications & cofactors

• domains:
• modifications:
• cofactors: Zn²⁺
• effectors:

⊟Localization

• PSORTb: Cytoplasmic
  • Cytoplasmic Score: 9.97
  • Cytoplasmic Membrane Score: 0
  • Cellwall Score: 0.01
  • Extracellular Score: 0.02
  • Internal Helices: 0
• LocateP: Intracellular
  • Prediction by SwissProt Classification: Cytoplasmic
  • Pathway Prediction: No pathway
  • Intracellular possibility: 1
  • Signal peptide possibility: -1
  • N-terminally Anchored Score: 1
  • Predicted Cleavage Site: No CleavageSite
• SignalP: no predicted signal peptide
  • SP(Sec/SPI): 0.063455
  • TAT(Tat/SPI): 0.000583
  • LIPO(Sec/SPII): 0.004528
• predicted transmembrane helices (TMHMM): 0

⊟Accession numbers

• GI: 15926207 NCBI
• RefSeq: NP_373740 NCBI
• UniProt: Q99W73 UniProt

⊟Protein sequence

• MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPAINYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGALNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSIDDLKVGARIDAGEHKEDALDFTLWKKAKPGEISWNSPFGEGRPGWHIECSVMAFHELGPTIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILVHDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATNIENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDKFKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQNIILEDTPQGVRFKRG

⊟Experimental data

• experimentally validated: data available for COL, NCTC8325
• protein localization: data available for COL
• quantitative data / protein copy number per cell: data available for COL
• interaction partners:

⊟Expression & Regulation

⊟Operon

• MicrobesOnline: cysE > cysS > SA0489 > SA0490 > SA0491 > SA0492

⊟Regulation

• regulator: T-box(Cys) (transcription antitermination) regulon

  T-box(Cys)

  (RNA)

  important in Amino acid metabolism; RegPrecise

⊟Transcription pattern

• S.aureus Expression Data Browser: data available for NCTC8325

⊟Protein synthesis (provided by Aureolib)

• Aureolib: data available for COL

⊟Protein stability

• half-life: no data available

⊟Biological Material

⊟Mutants

⊟Expression vector

⊟lacZ fusion

⊟GFP fusion

⊟two-hybrid system

⊟FLAG-tag construct

⊟Antibody

⊟Other Information

You are kindly invited to share additional interesting facts.

⊟Literature

⊟References

⊟Relevant publications

Alexander Scherl, Patrice François, Manuela Bento, Jacques M Deshusses, Yvan Charbonnier, Véronique Converset, Antoine Huyghe, Nadia Walter, Christine Hoogland, Ron D Appel, Jean-Charles Sanchez, Catherine G Zimmermann-Ivol, Garry L Corthals, Denis F Hochstrasser, Jacques Schrenzel
Correlation of proteomic and transcriptomic profiles of Staphylococcus aureus during the post-exponential phase of growth.
J Microbiol Methods: 2005, 60(2);247-57
[PubMed:15590099] [WorldCat.org] [DOI] (P p)