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NCBI: 10-JUN-2013

⊟Summary[edit | edit source]

  • organism: Staphylococcus aureus COL
  • locus tag: SACOL0535 [new locus tag: SACOL_RS02735 ]
  • pan locus tag?: SAUPAN002229000
  • symbol: SACOL0535
  • pan gene symbol?: rnmV
  • synonym:
  • product: hypothetical protein

⊟Genome View[edit | edit source]

⊟Gene[edit | edit source]

⊟General[edit | edit source]

  • type: CDS
  • locus tag: SACOL0535 [new locus tag: SACOL_RS02735 ]
  • symbol: SACOL0535
  • product: hypothetical protein
  • replicon: chromosome
  • strand: +
  • coordinates: 544988..545524
  • length: 537
  • essential: unknown other strains

⊟Accession numbers[edit | edit source]

⊟Phenotype[edit | edit source]

Share your knowledge and add information here. [edit]

⊟DNA sequence[edit | edit source]

  • 1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGAAAATCAATGAGTTTATAGTTGTAGAAGGACGAGATGATACTGAGCGTGTTAAACGA
    GCTGTTGAATGTGATACGATTGAAACGAATGGTAGTGCCATCAACGAACAAACTTTAGAA
    GTAATTAGAAATGCTCAACAAAGTCGAGGCGTTATTGTATTAACAGATCCAGATTTCCCA
    GGAGATAAAATTAGAAGTACAATTACTGAACATGTCAAAGGTGTTAAACATGCGTATATT
    GATAGAGAAAAAGCTAAAAATAAAAAAGGGAAAATTGGTGTTGAACATGCCGACTTAATT
    GATATTAAAGAAGCGTTAATGCATGTTAGTTCACCCTTTGATGAAGCTTATGAATCAATT
    GATAAATCTGTGCTAATAGAGTTGGGGTTAATTGTTGGGAAAGATGCAAGGCGCCGTAGA
    GAAATTTTAAGTAGAAAATTGCGAATCGGCCATTCCAATGGTAAGCAGTTATTGAAAAAG
    TTAAATGCATTTGGTTATACCGAAGCGGATGTAAGGCAAGCTTTAGAAGATGAATGA
    60
    120
    180
    240
    300
    360
    420
    480
    537

⊟Protein[edit | edit source]

⊟General[edit | edit source]

  • locus tag: SACOL0535 [new locus tag: SACOL_RS02735 ]
  • symbol: SACOL0535
  • description: hypothetical protein
  • length: 178
  • theoretical pI: 7.07882
  • theoretical MW: 20091.7
  • GRAVY: -0.57191

⊟Function[edit | edit source]

  • reaction:
    EC 3.1.26.8?  ExPASy
    Ribonuclease M5 Endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor
  • TIGRFAM:
    Genetic information processing Transcription RNA processing ribonuclease M5 (TIGR00334; EC 3.1.26.8; HMM-score: 179.9)
  • TheSEED  :
    • Ribonuclease M5 (EC 3.1.26.8)
    Protein Metabolism Protein biosynthesis Ribosome biogenesis bacterial  Ribonuclease M5 (EC 3.1.26.8)
  • PFAM:
    no clan defined DUF4093; Domain of unknown function (DUF4093) (PF13331; HMM-score: 86.7)
    and 6 more
    Toprim-like (CL0413) Toprim; Toprim domain (PF01751; HMM-score: 25)
    Toprim_4; Toprim domain (PF13662; HMM-score: 18.7)
    Toprim_2; Toprim-like (PF13155; HMM-score: 14.7)
    no clan defined pKID; pKID domain (PF02173; HMM-score: 12.7)
    STIR (CL0173) TIR-like; Predicted nucleotide-binding protein containing TIR-like domain (PF10137; HMM-score: 12.5)
    UBA (CL0214) UBA; UBA/TS-N domain (PF00627; HMM-score: 11.9)

⊟Structure, modifications & cofactors[edit | edit source]

  • domains:
  • modifications:
  • cofactors:
  • effectors:

⊟Localization[edit | edit source]

  • PSORTb: Cytoplasmic
    • Cytoplasmic Score: 7.5
    • Cytoplasmic Membrane Score: 1.15
    • Cellwall Score: 0.62
    • Extracellular Score: 0.73
    • Internal Helices: 0
  • LocateP: Intracellular
    • Prediction by SwissProt Classification: Cytoplasmic
    • Pathway Prediction: No pathway
    • Intracellular possibility: 1
    • Signal peptide possibility: -1
    • N-terminally Anchored Score: 1
    • Predicted Cleavage Site: No CleavageSite
  • SignalP: no predicted signal peptide
    • SP(Sec/SPI): 0.011313
    • TAT(Tat/SPI): 0.000658
    • LIPO(Sec/SPII): 0.00226
  • predicted transmembrane helices (TMHMM): 0

⊟Accession numbers[edit | edit source]

⊟Protein sequence[edit | edit source]

  • MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRNAQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHADLIDIKEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKKLNAFGYTEADVRQALEDE

⊟Experimental data[edit | edit source]

  • experimentally validated: PeptideAtlas
  • protein localization: Cytoplasmic [1]
  • quantitative data / protein copy number per cell: 81 [2]
  • interaction partners:

⊟Expression & Regulation[edit | edit source]

⊟Operon[edit | edit source]

⊟Regulation[edit | edit source]

  • regulator:

⊟Transcription pattern[edit | edit source]

⊟Protein synthesis (provided by Aureolib)[edit | edit source]

⊟Protein stability[edit | edit source]

  • half-life: no data available

⊟Biological Material[edit | edit source]

⊟Mutants[edit | edit source]

⊟Expression vector[edit | edit source]

⊟lacZ fusion[edit | edit source]

⊟GFP fusion[edit | edit source]

⊟two-hybrid system[edit | edit source]

⊟FLAG-tag construct[edit | edit source]

⊟Antibody[edit | edit source]

⊟Other Information[edit | edit source]

You are kindly invited to share additional interesting facts.

⊟Literature[edit | edit source]

⊟References[edit | edit source]

  1. ↑ Andreas Otto, Jan Maarten van Dijl, Michael Hecker, Dörte Becher
    The Staphylococcus aureus proteome.
    Int J Med Microbiol: 2014, 304(2);110-20
    [PubMed:24439828] [WorldCat.org] [DOI] (I p)
  2. ↑ Daniela Zühlke, Kirsten Dörries, Jörg Bernhardt, Sandra Maaß, Jan Muntel, Volkmar Liebscher, Jan Pané-Farré, Katharina Riedel, Michael Lalk, Uwe Völker, Susanne Engelmann, Dörte Becher, Stephan Fuchs, Michael Hecker
    Costs of life - Dynamics of the protein inventory of Staphylococcus aureus during anaerobiosis.
    Sci Rep: 2016, 6;28172
    [PubMed:27344979] [WorldCat.org] [DOI] (I e)

⊟Relevant publications[edit | edit source]

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